Detail

Contains

UniProtKB-O43707


Protein	Alpha-actinin-4
Gene	ACTN4
Status	Reviewed
Source	Colorectal tumors; LNCap/PC3 cell lines (Prostate cancer); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Prostate tumor; Urine
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
680	nGTLEDQLSHLK	2015(1);
680	ISIEMnGTLEDQLSHLK	2009(68); 2015(1);
726	HTnYTMEHIR	2007(unpublished);2015(unpublished);2015;2015;2007;2007;
821	IMSLVDPnHSGLVTFQAFIDFMSR	2015(2);

Sequence

1	11	21	31	41	51	61	71	81	91
MVDYHAANQS	YQYGPSSAGN	GAGGGGSMGD	YMAQEDDWDR	DLLLDPAWEK	QQRKTFTAWC	NSHLRKAGTQ	IENIDEDFRD	GLKLMLLLEV	ISGERLPKPE
101	111	121	131	141	151	161	171	181	191
RGKMRVHKIN	NVNKALDFIA	SKGVKLVSIG	AEEIVDGNAK	MTLGMIWTII	LRFAIQDISV	EETSAKEGLL	LWCQRKTAPY	KNVNVQNFHI	SWKDGLAFNA
201	211	221	231	241	251	261	271	281	291
LIHRHRPELI	EYDKLRKDDP	VTNLNNAFEV	AEKYLDIPKM	LDAEDIVNTA	RPDEKAIMTY	VSSFYHAFSG	AQKAETAANR	ICKVLAVNQE	NEHLMEDYEK
301	311	321	331	341	351	361	371	381	391
LASDLLEWIR	RTIPWLEDRV	PQKTIQEMQQ	KLEDFRDYRR	VHKPPKVQEK	CQLEINFNTL	QTKLRLSNRP	AFMPSEGKMV	SDINNGWQHL	EQAEKGYEEW
401	411	421	431	441	451	461	471	481	491
LLNEIRRLER	LDHLAEKFRQ	KASIHEAWTD	GKEAMLKHRD	YETATLSDIK	ALIRKHEAFE	SDLAAHQDRV	EQIAAIAQEL	NELDYYDSHN	VNTRCQKICD
501	511	521	531	541	551	561	571	581	591
QWDALGSLTH	SRREALEKTE	KQLEAIDQLH	LEYAKRAAPF	NNWMESAMED	LQDMFIVHTI	EEIEGLISAH	DQFKSTLPDA	DREREAILAI	HKEAQRIAES
601	611	621	631	641	651	661	671	681	691
NHIKLSGSNP	YTTVTPQIIN	SKWEKVQQLV	PKRDHALLEE	QSKQQSNEHL	RRQFASQANV	VGPWIQTKME	EIGRISIEMN	GTLEDQLSHL	KQYERSIVDY
701	711	721	731	741	751	761	771	781	791
KPNLDLLEQQ	HQLIQEALIF	DNKHTNYTME	HIRVGWEQLL	TTIARTINEV	ENQILTRDAK	GISQEQMQEF	RASFNHFDKD	HGGALGPEEF	KACLISLGYD
801	811	821	831	841	851	861	871	881	891
VENDRQGEAE	FNRIMSLVDP	NHSGLVTFQA	FIDFMSRETT	DTDTADQVIA	SFKVLAGDKN	FITAEELRRE	LPPDQAEYCI	ARMAPYQGPD	AVPGALDYKS
901	911
FSTALYGESD	L

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
68	Zhang L, Xu Y, Yao H, Xie L, Yao J, Lu H, Yang P: Boronic Acid Functionalized Core-Satellite Composite Nanoparticles for Advanced Enrichment of Glycopeptides and Glycoproteins. Chemistry-a European Journal 2009, 15:10158-10166.