Detail

UniProtKB-O60500


Protein	Nephrin
Gene	NPHS1
Status	Reviewed
Source	Breast cancer xenografts; Colorectal tumor; HEK293 cell line (embryonic kidney); Pancreatic islets; Urine
Years	2012-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
708	IWnVTR	2012(48); 2014(15); 2014(22); 2015(unpublished);2015(unpublished);2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MALGTTLRAS	LLLLGLLTEG	LAQLAIPASV	PRGFWALPEN	LTVVEGASVE	LRCGVSTPGS	AVQWAKDGLL	LGPDPRIPGF	PRYRLEGDPA	RGEFHLHIEA
101	111	121	131	141	151	161	171	181	191
CDLSDDAEYE	CQVGRSEMGP	ELVSPRVILS	ILVPPKLLLL	TPEAGTMVTW	VAGQEYVVNC	VSGDAKPAPD	ITILLSGQTI	SDISANVNEG	SQQKLFTVEA
201	211	221	231	241	251	261	271	281	291
TARVTPRSSD	NRQLLVCEAS	SPALEAPIKA	SFTVNVLFPP	GPPVIEWPGL	DEGHVRAGQS	LELPCVARGG	NPLATLQWLK	NGQPVSTAWG	TEHTQAVARS
301	311	321	331	341	351	361	371	381	391
VLVMTVRPED	HGAQLSCEAH	NSVSAGTQEH	GITLQVTFPP	SAIIILGSAS	QTENKNVTLS	CVSKSSRPRV	LLRWWLGWRQ	LLPMEETVMD	GLHGGHISMS
401	411	421	431	441	451	461	471	481	491
NLTFLARRED	NGLTLTCEAF	SEAFTKETFK	KSLILNVKYP	AQKLWIEGPP	EGQKLRAGTR	VRLVCLAIGG	NPEPSLMWYK	DSRTVTESRL	PQESRRVHLG
501	511	521	531	541	551	561	571	581	591
SVEKSGSTFS	RELVLVTGPS	DNQAKFTCKA	GQLSASTQLA	VQFPPTNVTI	LANASALRPG	DALNLTCVSV	SSNPPVNLSW	DKEGERLEGV	AAPPRRAPFK
601	611	621	631	641	651	661	671	681	691
GSAAARSVLL	QVSSRDHGQR	VTCRAHSAEL	RETVSSFYRL	NVLYRPEFLG	EQVLVVTAVE	QGEALLPVSV	SANPAPEAFN	WTFRGYRLSP	AGGPRHRILS
701	711	721	731	741	751	761	771	781	791
SGALHLWNVT	RADDGLYQLH	CQNSEGTAEA	RLRLDVHYAP	TIRALQDPTE	VNVGGSVDIV	CTVDANPILP	GMFNWERLGE	DEEDQSLDDM	EKISRGPTGR
801	811	821	831	841	851	861	871	881	891
LRIHHAKLAQ	AGAYQCIVDN	GVAPPARRLL	RLVVRFAPQV	EHPTPLTKVA	AAGDSTSSAT	LHCRARGVPN	IVFTWTKNGV	PLDLQDPRYT	EHTYHQGGVH
901	911	921	931	941	951	961	971	981	991
SSLLTIANVS	AAQDYALFTC	TATNALGSDQ	TNIQLVSISR	PDPPSGLKVV	SLTPHSVGLE	WKPGFDGGLP	QRFCIRYEAL	GTPGFHYVDV	VPPQATTFTL
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
TGLQPSTRYR	VWLLASNALG	DSGLADKGTQ	LPITTPGLHQ	PSGEPEDQLP	TEPPSGPSGL	PLLPVLFALG	GLLLLSNASC	VGGVLWQRRL	RRLAEGISEK
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
TEAGSEEDRV	RNEYEESQWT	GERDTQSSTV	STTEAEPYYR	SLRDFSPQLP	PTQEEVSYSR	GFTGEDEDMA	FPGHLYDEVE	RTYPPSGAWG	PLYDEVQMGP
1201	1211	1221	1231	1241
WDLHWPEDTY	QDPRGIYDQV	AGDLDTLEPD	SLPFELRGHL	V

Reference

ID	Publication
15	Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
22	Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.