Detail

UniProtKB-O75095


Protein	Multiple epidermal growth factor-like domains protein 6
Gene	MEGF6
Status	Reviewed
Source	HCC cell lines (Liver); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Prostate tumor; Serum
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
552	GWTGLICnE	2014(33);
961	nCTAGAACDAVNGSCLCPAGR	2015(1);
961	SACnCTAGAACDAVNGSCLCPAGR	2013(40); 2015(1); 2007(unpublished);2007(unpublished);2007(unpublished);
972	NCTAGAACDAVnGSCLCPAGR	2015(1);
972	SACNCTAGAACDAVnGSCLCPAGR	2013(40); 2015(1); 2007(unpublished);2007(unpublished);2007(unpublished);
998	GHnCSQACACF	2014(33);
1246	CPTGFLGTDCnLTCPQGR	2013(40);
1317	nGSCSCGLGWTGR	2015(1);
1352	YGAACHLECSCHnNSTCEPATGTCR	2013(40); 2014(33);
1353	nSTCEPATGTCR	2015(1);
1353	YGAACHLECSCHNnSTCEPATGTCR	2013(40); 2014(33);

Sequence

1	11	21	31	41	51	61	71	81	91
MSFLEEARAA	GRAVVLALVL	LLLPAVPVGA	SVPPRPLLPL	QPGMPHVCAE	QELTLVGRRQ	PCVQALSHTV	PVWKAGCGWQ	AWCVGHERRT	VYYMGYRQVY
101	111	121	131	141	151	161	171	181	191
TTEARTVLRC	CRGWMQQPDE	EGCLSAECSA	SLCFHGGRCV	PGSAQPCHCP	PGFQGPRCQY	DVDECRTHNG	GCQHRCVNTP	GSYLCECKPG	FRLHTDSRTC
201	211	221	231	241	251	261	271	281	291
LAINSCALGN	GGCQHHCVQL	TITRHRCQCR	PGFQLQEDGR	HCVRRSPCAN	RNGSCMHRCQ	VVRGLARCEC	HVGYQLAADG	KACEDVDECA	AGLAQCAHGC
301	311	321	331	341	351	361	371	381	391
LNTQGSFKCV	CHAGYELGAD	GRQCYRIEME	IVNSCEANNG	GCSHGCSHTS	AGPLCTCPRG	YELDTDQRTC	IDVDDCADSP	CCQQVCTNNP	GGYECGCYAG
401	411	421	431	441	451	461	471	481	491
YRLSADGCGC	EDVDECASSR	GGCEHHCTNL	AGSFQCSCEA	GYRLHEDRRG	CSPLEEPMVD	LDGELPFVRP	LPHIAVLQDE	LPQLFQDDDV	GADEEEAELR
501	511	521	531	541	551	561	571	581	591
GEHTLTEKFV	CLDDSFGHDC	SLTCDDCRNG	GTCLLGLDGC	DCPEGWTGLI	CNETCPPDTF	GKNCSFSCSC	QNGGTCDSVT	GACRCPPGVS	GTNCEDGCPK
601	611	621	631	641	651	661	671	681	691
GYYGKHCRKK	CNCANRGRCH	RLYGACLCDP	GLYGRFCHLT	CPPWAFGPGC	SEECQCVQPH	TQSCDKRDGS	CSCKAGFRGE	RCQAECELGY	FGPGCWQACT
701	711	721	731	741	751	761	771	781	791
CPVGVACDSV	SGECGKRCPA	GFQGEDCGQE	CPVGTFGVNC	SSSCSCGGAP	CHGVTGQCRC	PPGRTGEDCE	ADCPEGRWGL	GCQEICPACQ	HAARCDPETG
801	811	821	831	841	851	861	871	881	891
ACLCLPGFVG	SRCQDVCPAG	WYGPSCQTRC	SCANDGHCHP	ATGHCSCAPG	WTGFSCQRAC	DTGHWGPDCS	HPCNCSAGHG	SCDAISGLCL	CEAGYVGPRC
901	911	921	931	941	951	961	971	981	991
EQQCPQGHFG	PGCEQRCQCQ	HGAACDHVSG	ACTCPAGWRG	TFCEHACPAG	FFGLDCRSAC	NCTAGAACDA	VNGSCLCPAG	RRGPRCAETC	PAHTYGHNCS
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
QACACFNGAS	CDPVHGQCHC	APGWMGPSCL	QACPAGLYGD	NCRHSCLCQN	GGTCDPVSGH	CACPEGWAGL	ACEKECLPRD	VRAGCRHSGG	CLNGGLCDPH
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
TGRCLCPAGW	TGDKCQSPCL	RGWFGEACAQ	RCSCPPGAAC	HHVTGACRCP	PGFTGSGCEQ	ACPPGSFGED	CAQMCQCPGE	NPACHPATGT	CSCAAGYHGP
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
SCQQRCPPGR	YGPGCEQLCG	CLNGGSCDAA	TGACRCPTGF	LGTDCNLTCP	QGRFGPNCTH	VCGCGQGAAC	DPVTGTCLCP	PGRAGVRCER	GCPQNRFGVG
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
CEHTCSCRNG	GLCHASNGSC	SCGLGWTGRH	CELACPPGRY	GAACHLECSC	HNNSTCEPAT	GTCRCGPGFY	GQACEHPCPP	GFHGAGCQGL	CWCQHGAPCD
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
PISGRCLCPA	GFHGHFCERG	CEPGSFGEGC	HQRCDCDGGA	PCDPVTGLCL	CPPGRSGATC	NLDCRRGQFG	PSCTLHCDCG	GGADCDPVSG	QCHCVDGYMG
1501	1511	1521	1531	1541
PTCREGGPLR	LPENPSLAQG	SAGTLPASSR	PTSRSGGPAR	H

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
40	Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.