Detail

UniProtKB-O75197


Protein	Low-density lipoprotein receptor-related protein 5
Gene	LRP5
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; OVCAR-3 cell line (Ovarian cancer)
Years	2013-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
138	VANLnGTSR	2014(33);
138	IEVANInGTSR	2014(16);
138	IEVANLnGTSR	2013(40); 2014(13); 2014(33); 2015(1); 2015(2); 2015(unpublished);
138	IEVANInGTSRKV	2013(37);
705	AFMnGSSVEHVVE	2014(33);
940	TLDPSSRnCSPPTTF	2014(33);

Sequence

1	11	21	31	41	51	61	71	81	91
MEAAPPGPPW	PLLLLLLLLL	ALCGCPAPAA	ASPLLLFANR	RDVRLVDAGG	VKLESTIVVS	GLEDAAAVDF	QFSKGAVYWT	DVSEEAIKQT	YLNQTGAAVQ
101	111	121	131	141	151	161	171	181	191
NVVISGLVSP	DGLACDWVGK	KLYWTDSETN	RIEVANLNGT	SRKVLFWQDL	DQPRAIALDP	AHGYMYWTDW	GETPRIERAG	MDGSTRKIIV	DSDIYWPNGL
201	211	221	231	241	251	261	271	281	291
TIDLEEQKLY	WADAKLSFIH	RANLDGSFRQ	KVVEGSLTHP	FALTLSGDTL	YWTDWQTRSI	HACNKRTGGK	RKEILSALYS	PMDIQVLSQE	RQPFFHTRCE
301	311	321	331	341	351	361	371	381	391
EDNGGCSHLC	LLSPSEPFYT	CACPTGVQLQ	DNGRTCKAGA	EEVLLLARRT	DLRRISLDTP	DFTDIVLQVD	DIRHAIAIDY	DPLEGYVYWT	DDEVRAIRRA
401	411	421	431	441	451	461	471	481	491
YLDGSGAQTL	VNTEINDPDG	IAVDWVARNL	YWTDTGTDRI	EVTRLNGTSR	KILVSEDLDE	PRAIALHPVM	GLMYWTDWGE	NPKIECANLD	GQERRVLVNA
501	511	521	531	541	551	561	571	581	591
SLGWPNGLAL	DLQEGKLYWG	DAKTDKIEVI	NVDGTKRRTL	LEDKLPHIFG	FTLLGDFIYW	TDWQRRSIER	VHKVKASRDV	IIDQLPDLMG	LKAVNVAKVV
601	611	621	631	641	651	661	671	681	691
GTNPCADRNG	GCSHLCFFTP	HATRCGCPIG	LELLSDMKTC	IVPEAFLVFT	SRAAIHRISL	ETNNNDVAIP	LTGVKEASAL	DFDVSNNHIY	WTDVSLKTIS
701	711	721	731	741	751	761	771	781	791
RAFMNGSSVE	HVVEFGLDYP	EGMAVDWMGK	NLYWADTGTN	RIEVARLDGQ	FRQVLVWRDL	DNPRSLALDP	TKGYIYWTEW	GGKPRIVRAF	MDGTNCMTLV
801	811	821	831	841	851	861	871	881	891
DKVGRANDLT	IDYADQRLYW	TDLDTNMIES	SNMLGQERVV	IADDLPHPFG	LTQYSDYIYW	TDWNLHSIER	ADKTSGRNRT	LIQGHLDFVM	DILVFHSSRQ
901	911	921	931	941	951	961	971	981	991
DGLNDCMHNN	GQCGQLCLAI	PGGHRCGCAS	HYTLDPSSRN	CSPPTTFLLF	SQKSAISRMI	PDDQHSPDLI	LPLHGLRNVK	AIDYDPLDKF	IYWVDGRQNI
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
KRAKDDGTQP	FVLTSLSQGQ	NPDRQPHDLS	IDIYSRTLFW	TCEATNTINV	HRLSGEAMGV	VLRGDRDKPR	AIVVNAERGY	LYFTNMQDRA	AKIERAALDG
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
TEREVLFTTG	LIRPVALVVD	NTLGKLFWVD	ADLKRIESCD	LSGANRLTLE	DANIVQPLGL	TILGKHLYWI	DRQQQMIERV	EKTTGDKRTR	IQGRVAHLTG
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
IHAVEEVSLE	EFSAHPCARD	NGGCSHICIA	KGDGTPRCSC	PVHLVLLQNL	LTCGEPPTCS	PDQFACATGE	IDCIPGAWRC	DGFPECDDQS	DEEGCPVCSA
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
AQFPCARGQC	VDLRLRCDGE	ADCQDRSDEA	DCDAICLPNQ	FRCASGQCVL	IKQQCDSFPD	CIDGSDELMC	EITKPPSDDS	PAHSSAIGPV	IGIILSLFVM
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
GGVYFVCQRV	VCQRYAGANG	PFPHEYVSGT	PHVPLNFIAP	GGSQHGPFTG	IACGKSMMSS	VSLMGGRGGV	PLYDRNHVTG	ASSSSSSSTK	ATLYPPILNP
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
PPSPATDPSL	YNMDMFYSSN	IPATARPYRP	YIIRGMAPPT	TPCSTDVCDS	DYSASRWKAS	KYYLDLNSDS	DPYPPPPTPH	SQYLSAEDSC	PPSPATERSY
1601	1611
FHLFPPPPSP	CTDSS

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
13	Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40	Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.