Detail

Contains

UniProtKB-O75460


Protein	Serine/threonine-protein kinase/endoribonuclease IRE1
Gene	ERN1
Status	Reviewed
Source	HEK293 cell line (embryonic kidney); Liver; Pancreatic islets; Platelet
Years	2012-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
176	WnATYFDYAASLPEDDVDYK	2012(48); 2014(15); 2014(33); 2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MPARRLLLLL	TLLLPGLGIF	GSTSTVTLPE	TLLFVSTLDG	SLHAVSKRTG	SIKWTLKEDP	VLQVPTHVEE	PAFLPDPNDG	SLYTLGSKNN	EGLTKLPFTI
101	111	121	131	141	151	161	171	181	191
PELVQASPCR	SSDGILYMGK	KQDIWYVIDL	LTGEKQQTLS	SAFADSLCPS	TSLLYLGRTE	YTITMYDTKT	RELRWNATYF	DYAASLPEDD	VDYKMSHFVS
201	211	221	231	241	251	261	271	281	291
NGDGLVVTVD	SESGDVLWIQ	NYASPVVAFY	VWQREGLRKV	MHINVAVETL	RYLTFMSGEV	GRITKWKYPF	PKETEAKSKL	TPTLYVGKYS	TSLYASPSMV
301	311	321	331	341	351	361	371	381	391
HEGVAVVPRG	STLPLLEGPQ	TDGVTIGDKG	ECVITPSTDV	KFDPGLKSKN	KLNYLRNYWL	LIGHHETPLS	ASTKMLERFP	NNLPKHRENV	IPADSEKKSF
401	411	421	431	441	451	461	471	481	491
EEVINLVDQT	SENAPTTVSR	DVEEKPAHAP	ARPEAPVDSM	LKDMATIILS	TFLLIGWVAF	IITYPLSMHQ	QQQLQHQQFQ	KELEKIQLLQ	QQQQQLPFHP
501	511	521	531	541	551	561	571	581	591
PGDTAQDGEL	LDTSGPYSES	SGTSSPSTSP	RASNHSLCSG	SSASKAGSSP	SLEQDDGDEE	TSVVIVGKIS	FCPKDVLGHG	AEGTIVYRGM	FDNRDVAVKR
601	611	621	631	641	651	661	671	681	691
ILPECFSFAD	REVQLLRESD	EHPNVIRYFC	TEKDRQFQYI	AIELCAATLQ	EYVEQKDFAH	LGLEPITLLQ	QTTSGLAHLH	SLNIVHRDLK	PHNILISMPN
701	711	721	731	741	751	761	771	781	791
AHGKIKAMIS	DFGLCKKLAV	GRHSFSRRSG	VPGTEGWIAP	EMLSEDCKEN	PTYTVDIFSA	GCVFYYVISE	GSHPFGKSLQ	RQANILLGAC	SLDCLHPEKH
801	811	821	831	841	851	861	871	881	891
EDVIARELIE	KMIAMDPQKR	PSAKHVLKHP	FFWSLEKQLQ	FFQDVSDRIE	KESLDGPIVK	QLERGGRAVV	KMDWRENITV	PLQTDLRKFR	TYKGGSVRDL
901	911	921	931	941	951	961	971
LRAMRNKKHH	YRELPAEVRE	TLGSLPDDFV	CYFTSRFPHL	LAHTYRAMEL	CSHERLFQPY	YFHEPPEPQP	PVTPDAL

Reference

ID	Publication
15	Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.