Detail

Contains

UniProtKB-O76024


Protein	Wolframin
Gene	WFS1
Status	Reviewed
Source	Breast cancer xenografts; DLBCL cell lines (Diffuse large B-cell lymphoma); HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Prostate tumor
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
661	VYnSTLTWQQYGAL	2007(unpublished);2007(unpublished);
661	nSTLTWQQYGALCGPR	2015(1);
661	VYnSTITWQQYGAICGPR	2014(16);
661	VYnSTLTWQQYGALCGPR	2012(48);
746	nTSTAEEELCR	2015(1);
746	AYPACSPGnTSTAEEE	2014(33);
746	PACSPGnTSTAEEELCR	2012(48);
746	GEAYPACSPGnTSTAEEELCRL	2014(33);
746	CIYGEAYPACSPGnTSTAEEEICR	2014(16); 2014(32);
746	CLYGEAYPACSPGnTSTAEEELCR	2014(15); 2014(33); 2015(unpublished);2015(12);

Sequence

1	11	21	31	41	51	61	71	81	91
MDSNTAPLGP	SCPQPPPAPQ	PQARSRLNAT	ASLEQERSER	PRAPGPQAGP	GPGVRDAAAP	AEPQAQHTRS	RERADGTGPT	KGDMEIPFEE	VLERAKAGDP
101	111	121	131	141	151	161	171	181	191
KAQTEVGKHY	LQLAGDTDEE	LNSCTAVDWL	VLAAKQGRRE	AVKLLRRCLA	DRRGITSENE	REVRQLSSET	DLERAVRKAA	LVMYWKLNPK	KKKQVAVAEL
201	211	221	231	241	251	261	271	281	291
LENVGQVNEH	DGGAQPGPVP	KSLQKQRRML	ERLVSSESKN	YIALDDFVEI	TKKYAKGVIP	SSLFLQDDED	DDELAGKSPE	DLPLRLKVVK	YPLHAIMEIK
301	311	321	331	341	351	361	371	381	391
EYLIDMASRA	GMHWLSTIIP	THHINALIFF	FIVSNLTIDF	FAFFIPLVIF	YLSFISMVIC	TLKVFQDSKA	WENFRTLTDL	LLRFEPNLDV	EQAEVNFGWN
401	411	421	431	441	451	461	471	481	491
HLEPYAHFLL	SVFFVIFSFP	IASKDCIPCS	ELAVITGFFT	VTSYLSLSTH	AEPYTRRALA	TEVTAGLLSL	LPSMPLNWPY	LKVLGQTFIT	VPVGHLVVLN
501	511	521	531	541	551	561	571	581	591
VSVPCLLYVY	LLYLFFRMAQ	LRNFKGTYCY	LVPYLVCFMW	CELSVVILLE	STGLGLLRAS	IGYFLFLFAL	PILVAGLALV	GVLQFARWFT	SLELTKIAVT
601	611	621	631	641	651	661	671	681	691
VAVCSVPLLL	RWWTKASFSV	VGMVKSLTRS	SMVKLILVWL	TAIVLFCWFY	VYRSEGMKVY	NSTLTWQQYG	ALCGPRAWKE	TNMARTQILC	SHLEGHRVTW
701	711	721	731	741	751	761	771	781	791
TGRFKYVRVT	DIDNSAESAI	NMLPFFIGDW	MRCLYGEAYP	ACSPGNTSTA	EEELCRLKLL	AKHPCHIKKF	DRYKFEITVG	MPFSSGADGS	RSREEDDVTK
801	811	821	831	841	851	861	871	881
DIVLRASSEF	KSVLLSLRQG	SLIEFSTILE	GRLGSKWPVF	ELKAISCLNC	MAQLSPTRRH	VKIEHDWRST	VHGAVKFAFD	FFFFPFLSAA

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
12	Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
15	Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
32	Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.