Detail

UniProtKB-O94910


Protein	Adhesion G protein-coupled receptor L1
Gene	ADGRL1
Status	Reviewed
Source	Breast Cancer cell lines; HEK293 cell line (embryonic kidney); LNCap/PC3 cell lines (Prostate cancer); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Pancreatic islets; Serum
Years	2012-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
531	nCTSPWVNQVAQK	2015(1);
531	RGPDISnCTSPWV	2013(37);
531	GPDLSnCTSPWVNQVAQK	2012(48); 2014(15); 2014(18); 2015(1); 2015(2);
640	DMnATEQVHTATMLLDVLEEGAFLLADNVR	2015(2);
1249	EACGMDTLPLNGNFnNSYSLR	2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MARLAAVLWN	LCVTAVLVTS	ATQGLSRAGL	PFGLMRRELA	CEGYPIELRC	PGSDVIMVEN	ANYGRTDDKI	CDADPFQMEN	VQCYLPDAFK	IMSQRCNNRT
101	111	121	131	141	151	161	171	181	191
QCVVVAGSDA	FPDPCPGTYK	YLEVQYDCVP	YKVEQKVFVC	PGTLQKVLEP	TSTHESEHQS	GAWCKDPLQA	GDRIYVMPWI	PYRTDTLTEY	ASWEDYVAAR
201	211	221	231	241	251	261	271	281	291
HTTTYRLPNR	VDGTGFVVYD	GAVFYNKERT	RNIVKYDLRT	RIKSGETVIN	TANYHDTSPY	RWGGKTDIDL	AVDENGLWVI	YATEGNNGRL	VVSQLNPYTL
301	311	321	331	341	351	361	371	381	391
RFEGTWETGY	DKRSASNAFM	VCGVLYVLRS	VYVDDDSEAA	GNRVDYAFNT	NANREEPVSL	TFPNPYQFIS	SVDYNPRDNQ	LYVWNNYFVV	RYSLEFGPPD
401	411	421	431	441	451	461	471	481	491
PSAGPATSPP	LSTTTTARPT	PLTSTASPAA	TTPLRRAPLT	THPVGAINQL	GPDLPPATAP	VPSTRRPPAP	NLHVSPELFC	EPREVRRVQW	PATQQGMLVE
501	511	521	531	541	551	561	571	581	591
RPCPKGTRGI	ASFQCLPALG	LWNPRGPDLS	NCTSPWVNQV	AQKIKSGENA	ANIASELARH	TRGSIYAGDV	SSSVKLMEQL	LDILDAQLQA	LRPIERESAG
601	611	621	631	641	651	661	671	681	691
KNYNKMHKRE	RTCKDYIKAV	VETVDNLLRP	EALESWKDMN	ATEQVHTATM	LLDVLEEGAF	LLADNVREPA	RFLAAKENVV	LEVTVLNTEG	QVQELVFPQE
701	711	721	731	741	751	761	771	781	791
EYPRKNSIQL	SAKTIKQNSR	NGVVKVVFIL	YNNLGLFLST	ENATVKLAGE	AGPGGPGGAS	LVVNSQVIAA	SINKESSRVF	LMDPVIFTVA	HLEDKNHFNA
801	811	821	831	841	851	861	871	881	891
NCSFWNYSER	SMLGYWSTQG	CRLVESNKTH	TTCACSHLTN	FAVLMAHREI	YQGRINELLL	SVITWVGIVI	SLVCLAICIS	TFCFLRGLQT	DRNTIHKNLC
901	911	921	931	941	951	961	971	981	991
INLFLAELLF	LVGIDKTQYE	IACPIFAGLL	HYFFLAAFSW	LCLEGVHLYL	LLVEVFESEY	SRTKYYYLGG	YCFPALVVGI	AAAIDYRSYG	TEKACWLRVD
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
NYFIWSFIGP	VSFVIVVNLV	FLMVTLHKMI	RSSSVLKPDS	SRLDNIKSWA	LGAIALLFLL	GLTWAFGLLF	INKESVVMAY	LFTTFNAFQG	VFIFVFHCAL
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
QKKVHKEYSK	CLRHSYCCIR	SPPGGTHGSL	KTSAMRSNTR	YYTGTQSRIR	RMWNDTVRKQ	TESSFMAGDI	NSTPTLNRGT	MGNHLLTNPV	LQPRGGTSPY
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
NTLIAESVGF	NPSSPPVFNS	PGSYREPKHP	LGGREACGMD	TLPLNGNFNN	SYSLRSGDFP	PGDGGPEPPR	GRNLADAAAF	EKMIISELVH	NNLRGSSSAA
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
KGPPPPEPPV	PPVPGGGGEE	EAGGPGGADR	AEIELLYKAL	EEPLLLPRAQ	SVLYQSDLDE	SESCTAEDGA	TSRPLSSPPG	RDSLYASGAN	LRDSPSYPDS
1401	1411	1421	1431	1441	1451	1461	1471
SPEGPSEALP	PPPPAPPGPP	EIYYTSRPPA	LVARNPLQGY	YQVRRPSHEG	YLAAPGLEGP	GPDGDGQMQL	VTSL

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
15	Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
18	Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.