Contains      

UniProtKB-P00739

Protein Haptoglobin-related protein
Gene HPR
Status Reviewed
Source Bladder tumor; Breast cancer xenografts; Breast tumor; Cerebrospinal fluid; Colorectal tumor; HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HepG2 cell line (Liver); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); Lung tumor; Lymphocytes; Non-small Cell Lung Carcinoma; Ovarian tumor; Pancreatic islets; Plasma; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Serum (HCC); Spermatozoa; Urine; lung; ovarian tumor; plasma
Years 2004-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
126 MVSHHnLT 2015(unpublished);
126 nLTTGATL 2014(33);
126 HnLTTGATL 2014(33);
126 MVSHHnLTT 2014(33); 2015(unpublished);
126 HHnLTTGATL 2014(33);
126 MVSHHnLTTG 2014(33);
126 QAKMVSHHnL 2014(33);
126 VSHHnLTTGA 2014(33);
126 MVSHHnLTTGA 2014(33); 2015(unpublished);
126 SHHnLTTGATL 2014(33);
126 VSHHnLTTGAT 2009(64);
126 HnLTTGATLINE 2014(33);
126 MVSHHnLTTGAT 2014(14); 2014(33); 2015(unpublished);
126 VSHHnLTTGATL 2014(33);
126 HHnLTTGATLINE 2014(33);
126 MVSHHnLTTGATL 2014(21); 2014(33);
126 nLTTGATLINEQW 2014(33);
126 MVSHHnLTTGATLI 2014(14); 2014(33); 2015(unpublished);
126 SHHnLTTGATLINE 2014(33);
126 MVSHHnLTTGATLIN 2014(21); 2014(33); 2015(unpublished);
126 nLTTGATLINEQWLL 2014(33);
126 VSHHnLTTGATLINE 2014(33);
126 HnLTTGATLINEQWLL 2014(33);
126 MVSHHnLTTGATLINE 2014(33);
126 QAKMVSHHnLTTGATL 2014(33);
126 KMVSHHnLTTGATLINE 2014(33);
126 MVSHHnLTTGATLINEQ 2014(33); 2015(unpublished);
126 VSHHnLTTGATLINEQW 2014(33);
126 MVSHHnLTTGATLINEQW 2014(21); 2014(33);
126 MVSHHnLTTGATLINEQWL 2014(33); 2015(unpublished);
126 VSHHnLTTGATLINEQWLL 2014(33);
126 HnLTTGATLINEQWLLTTAK 2014(33); 2015(unpublished);
126 MVSHHnLTTGATLINEQWLL 2014(33); 2015(unpublished);
126 MVSHHnLTTGATLINEQWLLT 2014(33);
126 QAKMVSHHnLTTGATLINEQW 2014(33);
126 MVSHHnLTTGATLINEQWLLTT 2015(unpublished);
126 SHHnLTTGATLINEQWLLTTAK 2015(unpublished);
126 QAKMVSHHnLTTGATLINEQWLL 2014(33);
126 VSHHnLTTGATLINEQWLLTTAK 2014(33); 2015(unpublished);
126 MVSHHnITTGATIINEQWIITTAK 2013(43);
126 MVSHHnLTTGATLINEQWLLTTAK 2004( 82); 2005(79); 2005(80); 2005(81); 2006(76); 2007(71); 2007(69); 2008(64); 2009(66); 2009(60); 2010(40); 2010(56); 2011(49); 2012(53); 2012(34); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2013(45); 2013(13); 2014(35); 2014(15); 2014(18); 2014(22); 2014(24); 2014(26); 2014(28); 2014(30); 2014(31); 2014(33); 2014(5); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;2007;20072007 ;
126 MVSHHnLTTGATLINEQWLLTTAKN 2015(unpublished);
126 AKMVSHHnLTTGATLINEQWLLTTAK 2007(71);
126 GSFPWQAKMVSHHnLTTGATLINEQWLLTTAK 2015(unpublished);
126 MVSHHnLTTGATLINEQWLLTTAKNLFLNHSENATAK 2014(13); 2014(35); 2015(unpublished);
149 NLFLnH 2014(33);
149 NLFLnHS 2014(33); 2015(unpublished);
149 NLFLnHSE 2014(14); 2014(33); 2015(unpublished);
149 FLnHSENAT 2014(33);
149 nHSENATAK 2011(56); 2014(14); 2014(33);
149 NLFLnHSEN 2014(14); 2014(33); 2015(unpublished);
149 LnHSENATAK 2012(48); 2014(14); 2014(21); 2015(unpublished);
149 NLFLnHSENA 2014(14); 2014(33); 2015(unpublished);
149 FLnHSENATAK 2012(48); 2014(14); 2014(21); 2014(33); 2015(unpublished);
149 LnHSENATAKD 2009(64);
149 NLFLnHSENAT 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
149 FLnHSENATAKD 2014(33);
149 LFLnHSENATAK 2014(14); 2014(21); 2014(33); 2015(unpublished);2015(unpublished);
149 FLnHSENATAKDI 2014(33);
149 NIFInHSENATAK 2014(32);
149 NLFLnHSENATAK 2004( 82); 2005(79); 2005(80); 2005(81); 2006(76); 2006(77); 2007(71); 2007(73); 2007(69); 2008(64); 2009(66); 2009(60); 2010(40); 2010(54); 2011(55); 2011(3); 2012(48); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(38); 2013(39); 2013(40); 2013(42); 2013(45); 2013(45); 2013(14); 2014(35); 2014(18); 2014(21); 2014(22); 2014(24); 2014(26); 2014(28); 2014(31); 2014(33); 2014(5); 2015(7); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(UnpublishedUnpublished ); 2015;2007;2007;2007;2007;2007;2007;2007;20072007 ;
149 KNLFLnHSENATAK 2014(33); 2015(unpublished);
149 NLFLnHSENATAKD 2014(33); 2015(unpublished);
149 AKNLFLnHSENATAK 2014(33);
149 LnHSENATAKDIAPT 2014(33);
149 nHSENATAKDIAPTL 2014(33);
149 NLFLnHSENATAKDI 2015(unpublished);
149 FLnHSENATAKDIAPT 2014(33);
149 LnHSENATAKDIAPTL 2014(33);
149 FLnHSENATAKDIAPTL 2014(33);
149 LnHSENATAKDIAPTLT 2014(33);
149 TTAKNLFLnHSENATAK 2014(33);
149 FLnHSENATAKDIAPTLT 2014(33);
149 LFLnHSENATAKDIAPTL 2014(33);
149 nHSENATAKDIAPTLTLY 2014(33);
149 NLFLnHSENATAKDIAPT 2014(33);
149 FLnHSENATAKDIAPTLTL 2014(33);
149 LLTTAKNLFLnHSENATAK 2014(33);
149 LnHSENATAKDIAPTLTLY 2014(33);
149 NLFLnHSENATAKDIAPTL 2014(33);
149 FLnHSENATAKDIAPTLTLY 2014(33);
149 NLFLnHSENATAKDIAPTLT 2014(33);
149 LFLnHSENATAKDIAPTLTLY 2014(33);
149 NLFLnHSENATAKDIAPTLTL 2014(33);
149 NLFLnHSENATAKDIAPTLTLY 2014(33);
149 TTAKNLFLnHSENATAKDIAPTL 2014(33);
149 LLTTAKNLFLnHSENATAKDIAPTL 2014(33);
149 NLFLnHSENATAKDIAPTLTLYVGK 2012(53); 2013(38); 2013(38); 2013(42); 2014(13); 2014(35); 2014(33); 2015(unpublished);2015(unpublished);
149 NLFLnHSENATAKDIAPTLTLYVGKK 2008(69); 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2013(42); 2014(13); 2014(35); 2014(33); 2015(unpublished);2015(unpublished);
149 TTAKNLFLnHSENATAKDIAPTLTLY 2014(33);
149 LLTTAKNLFLnHSENATAKDIAPTLTLY 2014(33);
149 MVSHHNLTTGATLINEQWLLTTAKNLFLnHSENATAK 2014(13); 2014(35); 2015(unpublished);
153 HSEnATAK 2014(14); 2015(unpublished);
153 FLNHSEnAT 2014(33);
153 NHSEnATAK 2011(56); 2014(14); 2014(33);
153 LNHSEnATAK 2012(48); 2014(14); 2014(21); 2015(unpublished);
153 NLFLNHSEnA 2014(14); 2014(33); 2015(unpublished);
153 FLNHSEnATAK 2012(48); 2014(14); 2014(21); 2014(33); 2015(unpublished);
153 LNHSEnATAKD 2009(64);
153 NLFLNHSEnAT 2014(14); 2014(33); 2015(unpublished);2015(unpublished);

Sequence

1112131415161718191
MSDLGAVISLLLWGRQLFALYSGNDVTDISDDRFPKPPEIANGYVEHLFRYQCKNYYRLRTEGDGVYTLNDKKQWINKAVGDKLPECEAVCGKPKNPANP
101111121131141151161171181191
VQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYHQVDIGLIKLKQKVLV
201211221231241251261271281291
NERVMPICLPSKNYAEVGRVGYVSGWGQSDNFKLTDHLKYVMLPVADQYDCITHYEGSTCPKWKAPKSPVGVQPILNEHTFCVGMSKYQEDTCYGDAGSA
301311321331341
FAVHDLEEDTWYAAGILSFDKSCAVAEYGVYVKVTSIQHWVQKTIAEN

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
30Xu Y, Bailey U-M, Punyadeera C, Schulz BL: Identification of salivary N-glycoproteins and measurement of glycosylation site occupancy by boronate glycoprotein enrichment and liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry 2014, 28:471-482.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
49Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
73Larsen MR, Jensen SS, Jakobsen LA, Heegaard NHH: Exploring the sialiome using titanium dioxide chromatography and mass spectrometry. Molecular & Cellular Proteomics 2007, 6:1778-1787.
76Alvarez-Manilla G, Atwood J, Guo Y, Warren NL, Orlando R, Pierce M: Tools for glycoproteomic analysis: Size exclusion chromatography facilitates identification of tryptic glycopeptides with N-linked glycosylation sites. Journal of Proteome Research 2006, 5:701-708.
77Ramachandran P, Boontheung P, Xie YM, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. Journal of Proteome Research 2006, 5:1493-1503.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.