Contains      

UniProtKB-P01011

Protein Alpha-1-antichymotrypsin
Gene SERPINA3
Status Reviewed
Source Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal tumor; DRO-1 Cell Line (Thyroid Cance); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HepG2 cell line (Liver); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Plasma; Platelet; Prostate; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Serum (HCC); TPC-1 Cell Line (Thyroid Cance); Urine; ovarian tumor; plasma
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
33 PLDEEnLTQENQDR 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);
33 NSPLDEEnLTQENQDR 2005(81); 2007(unpublished);2007(73); 2014(14); 2014(28); 2015(unpublished);
33 PNSPLDEEnLTQENQDR 2014(14);
33 HPNSPLDEEnLTQENQDR 2014(28); 2014(33); 2015(unpublished);
93 nTTLTEILK 2007(unpublished);2014(33); 2015(unpublished);
93 HnTTLTEILK 2014(14);
93 LSLGAHnTTL 2009(64);
93 GAHnTTLTEIL 2014(33);
93 LSLGAHnTTLT 2014(33);
93 SLGAHnTTLTE 2014(33);
93 GAHnTTLTEILK 2014(33); 2015(unpublished);
93 LSLGAHnTTLTE 2014(33);
93 LGAHnTTLTEILK 2014(33); 2015(unpublished);
93 SLGAHnTTLTEIL 2014(33);
93 AFLSLGAHnTTLTE 2014(33);
93 GAHnTTLTEILKGL 2014(33);
93 LSLGAHnTTLTEIL 2014(33);
93 SLGAHnTTLTEILK 2012(48); 2014(21); 2014(33); 2015(unpublished);
93 LSLGAHnTTLTEILK 2010(60); 2012(48); 2014(14); 2014(33); 2015(unpublished);
93 FLSLGAHnTTLTEILK 2012(48); 2015(unpublished);
93 SLGAHnTTLTEILKGL 2014(33);
93 AFLSLGAHnTTLTEILK 2014(28); 2014(33); 2015(unpublished);
93 LSLGAHnTTLTEILKGL 2014(33);
93 LAFLSLGAHnTTLTEILK 2015(unpublished);
93 ALAFLSLGAHnTTLTEILK 2015(unpublished);
93 STALAFLSLGAHnTTLTEILK 2015(unpublished);
93 SISTALAFLSLGAHnTTLTEILK 2014(33); 2015(unpublished);
93 NVIFSPLSISTALAFLSLGAHnTTLTEILK 2005(81); 2010(59); 2012(53); 2014(13); 2014(35); 2014(15); 2014(24); 2014(26); 2015(unpublished);2015(unpublished);
93 APDKNVIFSPLSISTALAFLSLGAHnTTLTEILK 2010(59); 2012(53); 2013(38); 2014(13); 2014(35); 2014(31); 2014(33); 2015(unpublished);2015(unpublished);2015(11);
93 QLVLKAPDKNVIFSPLSISTALAFLSLGAHnTTLTEILK 2015(unpublished);
106 FnLTETSE 2014(33);
106 FnLTETSEAE 2014(33);
106 LKFnLTETSE 2009(64);
106 ILKGLKFnLTE 2013(42);
106 nLTETSEAEIH 2014(33);
106 FnLTETSEAEIH 2011(54); 2014(33); 2015(unpublished);
106 FnLTETSEAEIHQ 2014(33);
106 GLKFnLTETSEAE 2014(33);
106 KFnLTETSEAEIH 2014(33);
106 FnLTETSEAEIHQS 2015(unpublished);
106 KFnLTETSEAEIHQ 2014(33);
106 nLTETSEAEIHQSF 2014(33);
106 FnLTETSEAEIHQSF 2014(28); 2014(33);
106 FnLTETSEAEIHQSFQ 2014(33);
106 KFnLTETSEAEIHQSF 2014(33);
106 FnLTETSEAEIHQSFQH 2014(33); 2015(unpublished);
106 FnLTETSEAEIHQSFQHL 2012(48); 2015(unpublished);
106 FnLTETSEAEIHQSFQHLL 2012(48);
106 nLTETSEAEIHQSFQHLLR 2015(unpublished);
106 FnITETSEAEIHQSFQHIIR 2014(32);
106 FnLTETSEAEIHQSFQHLLR 2005( 79); 2005(80); 2005(81); 2007(62); 2009(62); 2009(64); 2009(59); 2010(40); 2010(48); 2012(52); 2012(53); 2012(38); 2013(38); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2013(13); 2014(35); 2014(24); 2014(26); 2014(28); 2014(31); 2014(33); 2014(1); 2015(7); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;2007;
106 GIKFnITETSEAEIHQSFQHIIR 2014(32); 2014(32);
106 GLKFnLTETSEAEIHQSFQHLLR 2005(79); 2005(80); 2008(69); 2012(53); 2013(38); 2013(38); 2013(45); 2013(45); 2014(13); 2014(35); 2014(15); 2014(31); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015(5); 2015(11);
106 FnLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVK 2013(38);
127 TLnQSSDE 2014(33);
127 LRTLnQSSDE 2009(64);
127 RTLnQSSDEL 2014(33);
127 LRTLnQSSDEL 2014(33);
127 TLnQSSDELQL 2014(14); 2014(33); 2015(unpublished);
127 LRTLnQSSDELQ 2009(64);
127 RTLnQSSDELQL 2014(33);
127 TLnQSSDELQLS 2014(33);
127 HIIRTInQSSDEI 2013(37);
127 LRTLnQSSDELQL 2014(33);
127 RTLnQSSDELQLS 2014(33);
127 TLnQSSDELQLSM 2014(33);
127 QHLLRTLnQSSDEL 2014(33);
127 RTLnQSSDELQLSM 2014(33);
127 TLnQSSDELQLSMGN 2014(14); 2014(21); 2014(33); 2015(unpublished);2015(unpublished);
127 QHLLRTLnQSSDELQL 2014(33);
127 TLnQSSDELQLSMGNAM 2014(14); 2015(unpublished);2015(unpublished);
127 IHQSFQHLLRTLnQSSDE 2013(42);
127 QHLLRTLnQSSDELQLSM 2014(33);
127 TLnQSSDELQLSMGNAMF 2014(21); 2014(33);
127 RTLnQSSDELQLSMGNAMF 2014(33);
127 LRTLnQSSDELQLSMGNAMF 2014(33);
127 TInQSSDEIQISMGNAMFVK 2014(32); 2014(32);
127 TLnQSSDELQLSMGNAMFVK 2005( 81); 2007(69); 2008(62); 2009(59); 2010(60); 2010(40); 2010(54); 2011(55); 2011(52); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(38); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(35); 2014(15); 2014(18); 2014(21); 2014(22); 2014(24); 2014(28); 2014(33); 2014(5); 2015(7); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;2007;2007;2007;
127 QHLLRTLnQSSDELQLSMGNAMF 2014(33);
127 TLnQSSDELQLSMGNAMFVKEQLSLLDR 2010(40); 2012(53); 2014(13); 2015(unpublished);
127 TLnQSSDELQLSMGNAMFVKEQLSLLDRFTEDAK 2014(13);
127 FNLTETSEAEIHQSFQHLLRTLnQSSDELQLSMGNAMFVK 2013(38);
186 INDYVKnGTR 2014(21); 2014(33);
186 LINDYVKnGTR 2005( 81); 2009(62); 2010(40); 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2014(14); 2014(21); 2014(24); 2014(31); 2014(33); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
186 KLINDYVKnGTR 2007(unpublished);2010(40); 2013(38); 2014(14); 2014(35); 2014(21); 2014(31); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
186 VKnGTRGKITDL 2014(33);
186 INDYVKnGTRGKI 2013(37);
186 INDYVKnGTRGKITDL 2014(33);
271 TGnASALF 2014(33);
271 KYTGnASAL 2009(64); 2014(33);
271 YTGnASALF 2014(33);
271 KYTGnASALF 2014(33);
271 LKYTGnASAL 2009(64); 2014(33);
271 LKYTGnASALF 2014(33);
271 EIKYTGnASAIFI 2013(37);
271 GnASALFILPDQDK 2014(14); 2015(unpublished);
271 LKYTGnASALFILPD 2013(42);
271 YTGnASALFILPDQDK 2005( 81); 2007(69); 2008(62); 2009(64); 2009(59); 2010(40); 2010(54); 2011(56); 2011(48); 2012(52); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2013(13); 2014(14); 2014(35); 2014(22); 2014(24); 2014(26); 2014(31); 2014(33); 2014(5); 2015(7); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;2007;2007;2007;
271 LKYTGnASALFILPDQDK 2014(33);
271 YTGnASALFILPDQDKMEE 2014(33);

Sequence

1112131415161718191
MERMLPLLALGLLAAGFCPAVLCHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEIL
101111121131141151161171181191
KGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLD
201211221231241251261271281291
SQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWR
301311321331341351361371381391
DSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFL
401411421
MIIVPTDTQNIFFMSKVTNPKQA

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
59Lee H-J, Na K, Choi E-Y, Kim KS, Kim H, Paik Y-K: Simple Method for Quantitative Analysis of N-Linked Glycoproteins in Hepatocellular Carcinoma Specimens. Journal of Proteome Research 2010, 9:308-318.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
73Larsen MR, Jensen SS, Jakobsen LA, Heegaard NHH: Exploring the sialiome using titanium dioxide chromatography and mass spectrometry. Molecular & Cellular Proteomics 2007, 6:1778-1787.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.