Contains      

UniProtKB-P01023

Protein Alpha-2-macroglobulin
Gene A2M
Status Reviewed
Source Bladder tumor; Breast cancer xenografts; Breast tumor; Cerebrospinal fluid; DRO-1 Cell Line (Thyroid Cance); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); HepG2 cell line (Liver); HuH-7 cell line (Liver); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Lung tumor; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Plasma; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate stromal cell line; Prostate tumor; Serum; Serum (Colorectal cancer); Serum (HCC); Urine; ovarian tumor; plasma
Years 2004-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
55 GCVLLSYLnE 2014(33);
55 KGCVLLSYLnE 2013(42); 2014(33);
55 TTEKGCVLLSYLnE 2014(33);
55 GCVLLSYLnETVTVSASLESVR 2005( 79); 2005(80); 2005(81); 2008(69); 2009(66); 2012(53); 2013(38); 2013(38); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(18); 2014(20); 2014(24); 2014(31); 2014(33); 2015(8); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(UnpublishedUnpublished ); 2007;2007;20072007 ;
55 GCVLLSYLnETVTVSASLESVRGNR 2008(69); 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2014(13); 2015(unpublished);2015(10);
70 GCVLLSYLNETVTVSASLESVRGnR 2008(69); 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2014(13); 2015(unpublished);2015(10);
70 GnRSLFTDLEAENDVLHCVAFAVPK 2013(38); 2013(38); 2013(38); 2013(38); 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);20072007 (UnpublishedUnpublished );
247 nVSVCGLY 2014(33);
247 MnVSVCGLY 2014(33);
247 IITILEEEMnV 2014(14);
247 nVSVCGLYTYGK 2014(33);
247 IITILEEEMnVSV 2014(14); 2015(unpublished);
247 MnVSVCGLYTYGK 2014(33);
247 IITILEEEMnVSVCGLY 2014(33);
247 IITILEEEMnVSVCGLYTYGK 2012(53); 2013(42); 2014(15); 2015(unpublished);
247 EMnVSVCGLYTYGKPVPGHVTVSICR 2007(unpublished);
247 IITILEEEMnVSVCGLYTYGKPVPGHVTVSICR 2005( 81); 2012(53); 2013(38); 2014(13); 2014(35); 2014(20); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(Unpublished ); 2007 ;
247 IITILEEEMnVSVCGLYTYGKPVPGHVTVSICRK 2015(unpublished);
396 ANYYSnATTDE 2013(42); 2014(33);
396 nATTDEHGLVQF 2014(33);
396 SnATTDEHGLVQ 2014(33);
396 SnATTDEHGLVQF 2012(49); 2014(33);
396 GNEANYYSnATTDE 2014(33);
396 YSnATTDEHGLVQF 2012(49); 2014(33);
396 GNEANYYSnATTDEHGLV 2014(14); 2015(unpublished);
396 IRGNEANYYSnATTDEHG 2009(64);
396 GNEANYYSnATTDEHGLVQ 2014(33);
396 NEANYYSnATTDEHGLVQF 2014(28);
396 GNEANYYSnATTDEHGLVQF 2011(54); 2014(21); 2014(28); 2014(33); 2015(unpublished);2015(5);
396 IRGNEANYYSnATTDEHGLVQF 2014(33);
396 GNEANYYSnATTDEHGIVQFSINTTNVMGTSITVR 2014(32); 2014(32);
396 GNEANYYSnATTDEHGLVQFSINTTNVMGTSLTVR 2005( 81); 2009(62); 2012(53); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(31); 2014(33); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;20072007 ;
396 GNEANYYSnATTDEHGLVQFSINTTNVMGTSLTVRVNYK 2013(38);
396 VIFIRGNEANYYSnATTDEHGLVQFSINTTNVMGTSLTVR 2013(38); 2013(38);
396 VIFIRGNEANYYSnATTDEHGLVQFSINTTNVMGTSLTVRVNYK 2013(38); 2013(38);
410 SInTTNVM 2014(33);
410 SInTTNVMGT 2014(33);
410 SInTTNVMGTSL 2012(49); 2014(33);
410 InTTNVMGTSLTVR 2014(21); 2014(33); 2015(7);
410 SInTTNVMGTSLTVR 2005(81); 2007(unpublished);2007(71); 2009(65); 2010(60); 2011(54); 2014(14); 2014(21); 2014(28); 2014(33); 2015(unpublished);2015(5);
410 HGLVQFSInTTNVMGTSL 2014(33);
410 GNEANYYSNATTDEHGIVQFSInTTNVMGTSITVR 2014(32); 2014(32);
410 GNEANYYSNATTDEHGLVQFSInTTNVMGTSLTVR 2005( 81); 2009(62); 2012(53); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(31); 2014(33); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;20072007 ;
410 GNEANYYSNATTDEHGLVQFSInTTNVMGTSLTVRVNYK 2013(38);
410 VIFIRGNEANYYSNATTDEHGLVQFSInTTNVMGTSLTVR 2013(38); 2013(38);
410 VIFIRGNEANYYSNATTDEHGLVQFSInTTNVMGTSLTVRVNYK 2013(38); 2013(38);
869 SLGNVnF 2014(33);
869 SLGNVnFTVS 2014(33);
869 AVTPKSLGNVnF 2014(33);
869 SLGNVnFTVSAE 2014(33);
869 SLGNVnFTVSAEALE 2014(33);
869 LGNVnFTVSAEALESQELCGTEVPSVPEHGR 2015(unpublished);
869 SIGNVnFTVSAEAIESQEICGTEVPSVPEHGR 2014(32);
869 SLGNVnFTVSAEALESQELCGTEVPSVPEHGR 2004( 82); 2005(81); 2008(69); 2009(62); 2010(60); 2010(40); 2012(53); 2013(34); 2013(40); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(17); 2014(20); 2014(24); 2014(28); 2014(33); 2015(1); 2015(2); 2015(5); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;20072007 ;
869 SLGNVnFTVSAEALESQELCGTEVPSVPEHGRK 2004(82); 2008(69); 2012(53); 2013(38); 2013(38); 2013(38); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(18); 2015(unpublished);2015(unpublished);
991 VLDYLnE 2014(33);
991 DYLnETQQL 2014(33);
991 IYVLDYLnE 2014(33);
991 LDYLnETQQ 2009(64);
991 NIYVLDYLnE 2014(33);
991 VLDYLnETQQ 2014(33);
991 nETQQLTPEIK 2014(33);
991 VLDYLnETQQL 2014(33);
991 APNIYVLDYLnE 2014(33);
991 DYLnETQQLTPEI 2014(33);
991 FAPNIYVLDYLnE 2014(33);
991 YLnETQQLTPEIK 2014(14); 2014(33);
991 DYLnETQQLTPEIK 2014(19); 2014(33); 2015(unpublished);
991 VLDYLnETQQLTPE 2014(33);
991 VLDYLnETQQLTPEI 2014(33);
991 VLFAPNIYVLDYLnE 2014(33);
991 VLDYLnETQQLTPEIK 2014(33); 2015(unpublished);2015(5);
991 IYVLDYLnETQQLTPEIK 2014(33); 2015(unpublished);
991 VLDYLnETQQLTPEIKSK 2014(33);
991 DYLnETQQLTPEIKSKAIGY 2014(33);
991 APNIYVLDYLnETQQLTPEIK 2014(33); 2015(unpublished);
991 FAPNIYVLDYLnETQQLTPEIK 2015(unpublished);
991 VLDYLnETQQLTPEIKSKAIGY 2014(33);
991 MVLFAPNIYVLDYLnETQQLTPEIK 2015(unpublished);
991 QNMVLFAPNIYVLDYLnETQQLTPEIK 2014(33);
1424 DKVSnQTL 2009(64); 2014(33);
1424 VSnQTLSL 2014(33);
1424 IYLDKVSnQ 2014(33);
1424 LDKVSnQTL 2014(33);
1424 VSnQTLSLF 2014(33);
1424 DKVSnQTLSL 2009(64);
1424 VSnQTLSLFF 2014(28); 2014(33);
1424 IYLDKVSnQTL 2014(33);
1424 DKVSnQTLSLFF 2014(33);
1424 LDKVSnQTLSLF 2014(33);
1424 IYLDKVSnQTLSL 2014(33);
1424 LDKVSnQTLSLFF 2014(33);
1424 VLIYLDKVSnQTL 2014(33);
1424 IYLDKVSnQTLSLF 2014(33);
1424 VSnQTLSLFFTVLQ 2014(33);
1424 nQTLSLFFTVLQDVPVR 2014(33);
1424 SnQTLSLFFTVLQDVPVR 2015(unpublished);
1424 VSnQTLSLFFTVLQDVPVR 2004( 82); 2005(79); 2005(80); 2005(81); 2007(71); 2007(66); 2009(59); 2010(60); 2010(40); 2010(53); 2012(38); 2013(38); 2013(38); 2013(38); 2013(42); 2013(13); 2014(14); 2014(35); 2014(15); 2014(24); 2014(26); 2014(31); 2014(33); 2014(5); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;20072007 ;
1424 VSnQTLSLFFTVLQDVPVRDLK 2013(38); 2013(38);
1424 VSnQTLSLFFTVLQDVPVRDLKPAIVK 2009(66); 2013(38); 2013(38); 2013(38); 2015(unpublished);2015(unpublished);

Sequence

1112131415161718191
MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEV
101111121131141151161171181191
MFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQ
201211221231241251261271281291
GSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCF
301311321331341351361371381391
YQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTD
401411421431441451461471481491
EHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIM
501511521531541551561571581591
AKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAV
601611621631641651661671681691
DQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGP
701711721731741751761771781791
EGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPY
801811821831841851861871881891
SVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVI
901911921931941951961971981991
KPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEI
1001101110211031104110511061107110811091
KSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVT
1101111111211131114111511161117111811191
LSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQ
1201121112211231124112511261127112811291
APSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLL
1301131113211331134113511361137113811391
QQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLE
14011411142114311441145114611471
RSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
49Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
59Lee H-J, Na K, Choi E-Y, Kim KS, Kim H, Paik Y-K: Simple Method for Quantitative Analysis of N-Linked Glycoproteins in Hepatocellular Carcinoma Specimens. Journal of Proteome Research 2010, 9:308-318.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.
82Hagglund P, Bunkenborg J, Elortza F, Jensen ON, Roepstorff P: A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation. Journal of Proteome Research 2004, 3:556-566.