Detail

UniProtKB-P01031


Protein	Complement C5
Gene	C5
Status	Reviewed
Source	HepG2 cell line (Liver); HuH-7 cell line (Liver); Human; Liver; Ovarian tumor; Plasma; Prostate tumor; Serum; Serum (HCC); Urine
Years	2005-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
741	AnISHK	2015(10);
741	AnISHKDMQLGR	2005( 81); 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2014(33); 2015(unpublished);2007(unpublished);2007;
741	CCVVASQLRAnISHKDMQLG	2015(6);
911	IGLHNInFSLE	2013(42); 2014(33);
911	VEGSSSHLVTFTVLPLEIGLHNInFSLETWFGK	2005( 81); 2014(35); 2015(unpublished);2007(unpublished);2007;
911	QKVEGSSSHLVTFTVLPLEIGLHNInFSLETWFGK	2015(unpublished);
1115	NYQLDnGSFK	2014(33);
1115	NYQLDnGSFKE	2013(42); 2014(33);
1115	YVEQNQNSICNSLLWLVENYQLDnGSFK	2014(20); 2015(unpublished);
1630	YnFSFR	2005( 81); 2007(42); 2013(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;

Sequence

1	11	21	31	41	51	61	71	81	91
MGLLGILCFL	IFLGKTWGQE	QTYVISAPKI	FRVGASENIV	IQVYGYTEAF	DATISIKSYP	DKKFSYSSGH	VHLSSENKFQ	NSAILTIQPK	QLPGGQNPVS
101	111	121	131	141	151	161	171	181	191
YVYLEVVSKH	FSKSKRMPIT	YDNGFLFIHT	DKPVYTPDQS	VKVRVYSLND	DLKPAKRETV	LTFIDPEGSE	VDMVEEIDHI	GIISFPDFKI	PSNPRYGMWT
201	211	221	231	241	251	261	271	281	291
IKAKYKEDFS	TTGTAYFEVK	EYVLPHFSVS	IEPEYNFIGY	KNFKNFEITI	KARYFYNKVV	TEADVYITFG	IREDLKDDQK	EMMQTAMQNT	MLINGIAQVT
301	311	321	331	341	351	361	371	381	391
FDSETAVKEL	SYYSLEDLNN	KYLYIAVTVI	ESTGGFSEEA	EIPGIKYVLS	PYKLNLVATP	LFLKPGIPYP	IKVQVKDSLD	QLVGGVPVTL	NAQTIDVNQE
401	411	421	431	441	451	461	471	481	491
TSDLDPSKSV	TRVDDGVASF	VLNLPSGVTV	LEFNVKTDAP	DLPEENQARE	GYRAIAYSSL	SQSYLYIDWT	DNHKALLVGE	HLNIIVTPKS	PYIDKITHYN
501	511	521	531	541	551	561	571	581	591
YLILSKGKII	HFGTREKFSD	ASYQSINIPV	TQNMVPSSRL	LVYYIVTGEQ	TAELVSDSVW	LNIEEKCGNQ	LQVHLSPDAD	AYSPGQTVSL	NMATGMDSWV
601	611	621	631	641	651	661	671	681	691
ALAAVDSAVY	GVQRGAKKPL	ERVFQFLEKS	DLGCGAGGGL	NNANVFHLAG	LTFLTNANAD	DSQENDEPCK	EILRPRRTLQ	KKIEEIAAKY	KHSVVKKCCY
701	711	721	731	741	751	761	771	781	791
DGACVNNDET	CEQRAARISL	GPRCIKAFTE	CCVVASQLRA	NISHKDMQLG	RLHMKTLLPV	SKPEIRSYFP	ESWLWEVHLV	PRRKQLQFAL	PDSLTTWEIQ
801	811	821	831	841	851	861	871	881	891
GVGISNTGIC	VADTVKAKVF	KDVFLEMNIP	YSVVRGEQIQ	LKGTVYNYRT	SGMQFCVKMS	AVEGICTSES	PVIDHQGTKS	SKCVRQKVEG	SSSHLVTFTV
901	911	921	931	941	951	961	971	981	991
LPLEIGLHNI	NFSLETWFGK	EILVKTLRVV	PEGVKRESYS	GVTLDPRGIY	GTISRRKEFP	YRIPLDLVPK	TEIKRILSVK	GLLVGEILSA	VLSQEGINIL
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
THLPKGSAEA	ELMSVVPVFY	VFHYLETGNH	WNIFHSDPLI	EKQKLKKKLK	EGMLSIMSYR	NADYSYSVWK	GGSASTWLTA	FALRVLGQVN	KYVEQNQNSI
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
CNSLLWLVEN	YQLDNGSFKE	NSQYQPIKLQ	GTLPVEAREN	SLYLTAFTVI	GIRKAFDICP	LVKIDTALIK	ADNFLLENTL	PAQSTFTLAI	SAYALSLGDK
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
THPQFRSIVS	ALKREALVKG	NPPIYRFWKD	NLQHKDSSVP	NTGTARMVET	TAYALLTSLN	LKDINYVNPV	IKWLSEEQRY	GGGFYSTQDT	INAIEGLTEY
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
SLLVKQLRLS	MDIDVSYKHK	GALHNYKMTD	KNFLGRPVEV	LLNDDLIVST	GFGSGLATVH	VTTVVHKTST	SEEVCSFYLK	IDTQDIEASH	YRGYGNSDYK
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
RIVACASYKP	SREESSSGSS	HAVMDISLPT	GISANEEDLK	ALVEGVDQLF	TDYQIKDGHV	ILQLNSIPSS	DFLCVRFRIF	ELFEVGFLSP	ATFTVYEYHR
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
PDKQCTMFYS	TSNIKIQKVC	EGAACKCVEA	DCGQMQEELD	LTISAETRKQ	TACKPEIAYA	YKVSITSITV	ENVFVKYKAT	LLDIYKTGEA	VAEKDSEITF
1601	1611	1621	1631	1641	1651	1661	1671
IKKVTCTNAE	LVKGRQYLIM	GKEALQIKYN	FSFRYIYPLD	SLTWIEYWPR	DTTCSSCQAF	LANLDEFAED	IFLNGC

Reference

ID	Publication
6	Kim DS, Hahn Y: The acquisition of novel N-glycosylation sites in conserved proteins during human evolution. Bmc Bioinformatics 2015, 16.
10	Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
20	Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
35	Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38	Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
42	Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
53	Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.