Contains      

UniProtKB-P01871

Protein Ig mu chain C region
Gene IGHM
Status Reviewed
Source Bladder tumor; Breast tumor; Cerebrospinal fluid; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); Liver; Liver tumor (HCC); Lymphocytes; Ovarian tumor; Plasma; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Urine; lung; plasma
Years 2005-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
46 nNSDISSTR 2005( 81); 2007(71); 2009(66); 2012(53); 2013(45); 2014(13); 2014(14); 2014(22); 2014(26); 2014(33); 2015(10); 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);
46 YKnNSDISSTR 2005(79); 2005(80); 2007(unpublished);2007(71); 2008(69); 2009(64); 2009(66); 2012(3); 2012(53); 2013(34); 2013(39); 2013(42); 2013(45); 2013(45); 2014(13); 2014(16); 2014(21); 2014(22); 2014(26); 2014(28); 2014(31); 2014(32); 2014(32); 2014(33); 2015(unpublished);2015(11);
46 KYKnNSDISSTR 2014(33);
46 WKYKnNSDISSTR 2007(71);
46 KnNSDISSTRGFPSVL 2014(33);
46 KYKnNSDISSTRGFPSVL 2014(33);
209 TFQQnASSM 2014(33);
209 GLTFQQnASSM 2014(27); 2014(33);
209 nASSMCVPDQDTAIR 2014(33);
209 QnASSMCVPDQDTAIR 2014(33);
209 GLTFQQnASSMCVPDQD 2014(33);
209 QQnASSMCVPDQDTAIR 2014(33);
209 QQnASSMCVPDQDTAIRVF 2014(33);
209 GITFQQnASSMCVPDQDTAIR 2014(16); 2014(32); 2014(32);
209 GLTFQQnASSMCVPDQDTAIR 2005( 81); 2007(71); 2007(73); 2007(69); 2008(66); 2009(60); 2010(40); 2010(3); 2012(53); 2012(34); 2013(42); 2013(45); 2013(13); 2014(14); 2014(35); 2014(20); 2014(21); 2014(22); 2014(24); 2014(26); 2014(28); 2014(31); 2014(33); 2014(5); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(UnpublishedUnpublished ); 2007;20072007 ;
209 TFQQnASSMCVPDQDTAIRVF 2014(33);
209 VDHRGLTFQQnASSMCVPDQDTAIR 2014(13);
209 GLTFQQnASSMCVPDQDTAIRVFAIPPSFASIFLTK 2012(53);
272 THTnISE 2014(33);
272 AVKTHTnISE 2013(42); 2014(33);
272 TnISESHPNATF 2014(33);
272 THTnISESHPNAT 2012(3); 2014(14); 2014(27); 2014(33); 2015(unpublished);
272 THTnISESHPNATF 2007(unpublished);2014(27); 2014(28); 2014(33);
272 THTnISESHPNATFS 2014(27);
272 THTnISESHPNATFSAVG 2014(27);
272 THTnISESHPNATFSAVGE 2014(27); 2014(33);
272 QNGEAVKTHTnISESHPNATF 2014(33);
272 TRQNGEAVKTHTnISESHPNATF 2014(33);
272 THTnISESHPNATFSAVGEASICEDDWNSGER 2005( 81); 2007(71); 2008(69); 2010(40); 2012(53); 2013(42); 2013(45); 2014(13); 2014(35); 2014(16); 2014(20); 2014(33); 2015(5); 2015(10); 2015(11); 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);
279 ISESHPnAT 2009(64);
279 SHPnATFSAVGE 2013(42); 2014(33);
279 TNISESHPnATF 2014(33);
279 THTNISESHPnAT 2012(3); 2014(14); 2014(27); 2014(33); 2015(unpublished);
279 THTNISESHPnATF 2007(unpublished);2014(27); 2014(28); 2014(33);
279 THTNISESHPnATFS 2014(27);
279 THTNISESHPnATFSAVG 2014(27);
279 THTNISESHPnATFSAVGE 2014(27); 2014(33);
279 QNGEAVKTHTNISESHPnATF 2014(33);
279 TRQNGEAVKTHTNISESHPnATF 2014(33);
279 THTNISESHPnATFSAVGEASICEDDWNSGER 2005( 81); 2007(71); 2008(69); 2010(40); 2012(53); 2013(42); 2013(45); 2014(13); 2014(35); 2014(16); 2014(20); 2014(33); 2015(5); 2015(10); 2015(11); 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);
439 STGKPTLYnVSL 2007(71);
439 nVSLVMSDTAGTC 2014(33);
439 nVSLVMSDTAGTCY 2014(33);
439 STGKPTLYnVSLVM 2007(71);
439 STGKPTLYnVSLVMS 2014(28);
439 PTLYnVSLVMSDTAGTCY 2015(8);
439 RTVDKSTGKPTLYnVSLVMSD 2013(42);
439 STGKPTLYnVSLVMSDTAGTC 2007(unpublished);2014(33);
439 STGKPTLYnVSLVMSDTAGTCY 2005(81); 2007(unpublished);2008(69); 2010(40); 2012(53); 2013(34); 2013(42); 2013(45); 2014(13); 2014(35); 2014(24); 2014(26); 2014(33);
439 TVDKSTGKPTIYnVSIVMSDTAGTCY 2014(16);
439 TVDKSTGKPTLYnVSLVMSDTAGTCY 2012(53); 2014(33);
439 RTVDKSTGKPTLYnVSLVMSDTAGTCY 2013(42);

Sequence

1112131415161718191
GSASAPTLFPLVSCENSPSDTSSVAVGCLAQDFLPDSITLSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKN
101111121131141151161171181191
VPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVD
201211221231241251261271281291
HRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGER
301311321331341351361371381391
FTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTV
401411421431441451
SEEEWNTGETYTCVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
27Wang J, Zhou C, Zhang W, Yao J, Lu HJ, Dong QZ, Zhou HJ, Qin LX: An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples. Proteome Science 2014, 12.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
73Larsen MR, Jensen SS, Jakobsen LA, Heegaard NHH: Exploring the sialiome using titanium dioxide chromatography and mass spectrometry. Molecular & Cellular Proteomics 2007, 6:1778-1787.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.