Contains      

UniProtKB-P01877

Protein Ig alpha-2 chain C region
Gene IGHA2
Status Reviewed
Source Bladder tumor; Breast Cancer cell lines; Breast tumor; Cerebrospinal fluid; Colorectal tumor; HEK293 cell line (embryonic kidney); Liver; Ovarian tumor; Plasma; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Urine; lung; milk; ovarian tumor; plasma
Years 2004-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
47 SGQnVTAR 2014(33);
47 SESGQnVTAR 2007(3); 2012(14); 2014(33); 2014(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;
47 SVTWSESGQnVTAR 2005(79); 2005(80); 2007(71); 2011(54); 2014(27); 2014(28); 2014(33);
47 SGQnVTARNFPPSQD 2013(42);
47 PQEPLSVTWSESGQnVTAR 2005(81);
47 SGQnVTARNFPPSQDASGD 2013(42);
47 VTWSESGQnVTARNFPPSQD 2009(64);
47 SESGQnVTARNFPPSQDASGDLY 2014(33);
47 VQGFFPQEPLSVTWSESGQnVTAR 2014(33);
47 LVQGFFPQEPLSVTWSESGQnVTAR 2010(60); 2014(28);
47 VFPLSLDSTPQDGNVVVACLVQGFFPQEPLSVTWSESGQnVTAR 2008(70); 2012(53); 2014(35); 2014(15); 2014(30); 2015(11);
131 AnLTCTL 2014(33);
131 AnLTCTLT 2014(33);
131 GSEAnLTCTL 2014(33);
131 nLTCTLTGLR 2014(14);
131 AnLTCTLTGLR 2014(33);
131 DLLLGSEAnLT 2014(33);
131 AnLTCTLTGLRD 2013(42);
131 EAnLTCTLTGLR 2014(33);
131 SEAnLTCTLTGLR 2014(33);
131 GSEAnLTCTLTGLR 2014(33);
131 PALEDLLLGSEAnL 2014(33);
131 PALEDLLLGSEAnLT 2014(14); 2015(unpublished);
131 PALEDLLLGSEAnLTC 2014(33);
131 PALEDLLLGSEAnLTCT 2014(33);
131 DLLLGSEAnLTCTLTGLR 2014(28); 2014(33);
131 PALEDLLLGSEAnLTCTL 2014(33);
131 PALEDLLLGSEAnLTCTLT 2014(33);
131 HRPALEDLLLGSEAnLTCTL 2014(33);
131 PALEDLLLGSEAnLTCTLTGLR 2004(82); 2005(81); 2009(64); 2009(66); 2012(53); 2013(42); 2014(14); 2014(15); 2014(26); 2014(33); 2015(unpublished);2015(8); 2015(11);
131 SLHRPALEDLLLGSEAnLTCTL 2014(33);
131 LSLHRPALEDLLLGSEAnLTCTL 2014(28); 2014(33);
131 HRPALEDLLLGSEAnLTCTLTGLR 2010(60);
131 LSLHRPALEDLLLGSEAnLTCTLT 2014(33);
131 LSLHRPALEDLLLGSEAnLTCTLTGLR 2004( 82); 2005(79); 2005(80); 2008(69); 2008(70); 2009(64); 2009(66); 2010(40); 2012(51); 2012(53); 2013(34); 2013(42); 2013(45); 2013(45); 2014(13); 2014(35); 2014(20); 2014(26); 2014(30); 2014(31); 2014(33); 2015(5); 2015(8); 2015(10); 2015(11); 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);20072007 (UnpublishedUnpublished );
205 PLTAnITK 2014(14); 2014(33); 2015(unpublished);
205 TPLTAnITK 2005( 79); 2005(80); 2005(81); 2006(77); 2007(71); 2007(69); 2008(70); 2008(66); 2009(60); 2010(40); 2010(54); 2011(55); 2011(3); 2012(53); 2012(34); 2013(39); 2013(42); 2013(45); 2013(13); 2014(14); 2014(21); 2014(22); 2014(26); 2014(27); 2014(28); 2014(30); 2014(33); 2014(7); 2015(8); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;
205 LKTPLTAnITK 2014(33);
205 TAnITKSGNTF 2014(33);
205 TPLTAnITKSGN 2014(33);
205 KTPITAnITKSGN 2013(37);
205 TPLTAnITKSGNTF 2014(33);
205 KTPLTAnITKSGNTF 2009(64);
205 TPLTAnITKSGNTFRPE 2014(33);
205 LKTPLTAnITKSGNTFRPE 2013(42);
205 TPLTAnITKSGNTFRPEVH 2014(33);
205 KTPLTAnITKSGNTFRPEVHL 2009(64);
327 HVnVSVVMAE 2014(33);
327 PTHVnVSVVM 2014(33);
327 AGKPTHVnVSV 2014(33);
327 PTHVnVSVVMAE 2014(33);
327 GKPTHVnVSVVMA 2013(37);
327 HVnVSVVMAEVDGTC 2014(33);
327 HVnVSVVMAEVDGTCY 2014(33);
327 AGKPTHVnVSVVMAEVDG 2014(33);
327 PTHVnVSVVMAEVDGTCY 2009(66); 2013(42); 2014(33); 2015(8);
327 AGKPTHVnVSVVMAEVDGTC 2014(33);
327 AGKPTHVnVSVVMAEVDGTCY 2014(33);
327 MAGKPTHVnVSVVMAEVDGTCY 2013(42); 2014(13); 2014(35); 2015(8);

Sequence

1112131415161718191
ASPTSPKVFPLSLDSTPQDGNVVVACLVQGFFPQEPLSVTWSESGQNVTARNFPPSQDASGDLYTTSSQLTLPATQCPDGKSVTCHVKHYTNPSQDVTVP
101111121131141151161171181191
CPVPPPPPCCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGATFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKT
201211221231241251261271281291
PLTANITKSGNTFRPEVHLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDTFSC
301311321331
MVGHEALPLAFTQKTIDRMAGKPTHVNVSVVMAEVDGTCY

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
27Wang J, Zhou C, Zhang W, Yao J, Lu HJ, Dong QZ, Zhou HJ, Qin LX: An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples. Proteome Science 2014, 12.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
30Xu Y, Bailey U-M, Punyadeera C, Schulz BL: Identification of salivary N-glycoproteins and measurement of glycosylation site occupancy by boronate glycoprotein enrichment and liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry 2014, 28:471-482.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
51Yeh C-H, Chen S-H, Li D-T, Lin H-P, Huang H-J, Chang C-I, Shih W-L, Chern C-L, Shi F-K, Hsu J-L: Magnetic bead-based hydrophilic interaction liquid chromatography for glycopeptide enrichments. Journal of Chromatography A 2012, 1224:70-78.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
70Picariello G, Ferranti P, Mamone G, Roepstorff P, Addeo F: Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry. Proteomics 2008, 8:3833-3847.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
77Ramachandran P, Boontheung P, Xie YM, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. Journal of Proteome Research 2006, 5:1493-1503.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.
82Hagglund P, Bunkenborg J, Elortza F, Jensen ON, Roepstorff P: A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation. Journal of Proteome Research 2004, 3:556-566.