Detail

UniProtKB-P02458


Protein	Collagen alpha-1(II) chain
Gene	COL2A1
Status	Reviewed
Source	Breast Cancer cell lines; DLBCL cell lines (Diffuse large B-cell lymphoma); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Plasma; Prostate
Years	2013-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
317	nGSPGPMGPR	2015(1);
1388	nITYHCK	2015(1);
1388	EGSQnITYHCK	2015(unpublished);
1388	STEGSQnITYHCK	2013(37);
1388	IISTEGSQnITYHCK	2014(16);
1388	LLSTEGSQnITYHCK	2014(13); 2014(14); 2014(18);

Sequence

1	11	21	31	41	51	61	71	81	91
MIRLGAPQTL	VLLTLLVAAV	LRCQGQDVQE	AGSCVQDGQR	YNDKDVWKPE	PCRICVCDTG	TVLCDDIICE	DVKDCLSPEI	PFGECCPICP	TDLATASGQP
101	111	121	131	141	151	161	171	181	191
GPKGQKGEPG	DIKDIVGPKG	PPGPQGPAGE	QGPRGDRGDK	GEKGAPGPRG	RDGEPGTPGN	PGPPGPPGPP	GPPGLGGNFA	AQMAGGFDEK	AGGAQLGVMQ
201	211	221	231	241	251	261	271	281	291
GPMGPMGPRG	PPGPAGAPGP	QGFQGNPGEP	GEPGVSGPMG	PRGPPGPPGK	PGDDGEAGKP	GKAGERGPPG	PQGARGFPGT	PGLPGVKGHR	GYPGLDGAKG
301	311	321	331	341	351	361	371	381	391
EAGAPGVKGE	SGSPGENGSP	GPMGPRGLPG	ERGRTGPAGA	AGARGNDGQP	GPAGPPGPVG	PAGGPGFPGA	PGAKGEAGPT	GARGPEGAQG	PRGEPGTPGS
401	411	421	431	441	451	461	471	481	491
PGPAGASGNP	GTDGIPGAKG	SAGAPGIAGA	PGFPGPRGPP	GPQGATGPLG	PKGQTGEPGI	AGFKGEQGPK	GEPGPAGPQG	APGPAGEEGK	RGARGEPGGV
501	511	521	531	541	551	561	571	581	591
GPIGPPGERG	APGNRGFPGQ	DGLAGPKGAP	GERGPSGLAG	PKGANGDPGR	PGEPGLPGAR	GLTGRPGDAG	PQGKVGPSGA	PGEDGRPGPP	GPQGARGQPG
601	611	621	631	641	651	661	671	681	691
VMGFPGPKGA	NGEPGKAGEK	GLPGAPGLRG	LPGKDGETGA	AGPPGPAGPA	GERGEQGAPG	PSGFQGLPGP	PGPPGEGGKP	GDQGVPGEAG	APGLVGPRGE
701	711	721	731	741	751	761	771	781	791
RGFPGERGSP	GAQGLQGPRG	LPGTPGTDGP	KGASGPAGPP	GAQGPPGLQG	MPGERGAAGI	AGPKGDRGDV	GEKGPEGAPG	KDGGRGLTGP	IGPPGPAGAN
801	811	821	831	841	851	861	871	881	891
GEKGEVGPPG	PAGSAGARGA	PGERGETGPP	GPAGFAGPPG	ADGQPGAKGE	QGEAGQKGDA	GAPGPQGPSG	APGPQGPTGV	TGPKGARGAQ	GPPGATGFPG
901	911	921	931	941	951	961	971	981	991
AAGRVGPPGS	NGNPGPPGPP	GPSGKDGPKG	ARGDSGPPGR	AGEPGLQGPA	GPPGEKGEPG	DDGPSGAEGP	PGPQGLAGQR	GIVGLPGQRG	ERGFPGLPGP
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
SGEPGKQGAP	GASGDRGPPG	PVGPPGLTGP	AGEPGREGSP	GADGPPGRDG	AAGVKGDRGE	TGAVGAPGAP	GPPGSPGPAG	PTGKQGDRGE	AGAQGPMGPS
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
GPAGARGIQG	PQGPRGDKGE	AGEPGERGLK	GHRGFTGLQG	LPGPPGPSGD	QGASGPAGPS	GPRGPPGPVG	PSGKDGANGI	PGPIGPPGPR	GRSGETGPAG
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
PPGNPGPPGP	PGPPGPGIDM	SAFAGLGPRE	KGPDPLQYMR	ADQAAGGLRQ	HDAEVDATLK	SLNNQIESIR	SPEGSRKNPA	RTCRDLKLCH	PEWKSGDYWI
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
DPNQGCTLDA	MKVFCNMETG	ETCVYPNPAN	VPKKNWWSSK	SKEKKHIWFG	ETINGGFHFS	YGDDNLAPNT	ANVQMTFLRL	LSTEGSQNIT	YHCKNSIAYL
1401	1411	1421	1431	1441	1451	1461	1471	1481
DEAAGNLKKA	LLIQGSNDVE	IRAEGNSRFT	YTALKDGCTK	HTGKWGKTVI	EYRSQKTSRL	PIIDIAPMDI	GGPEQEFGVD	IGPVCFL

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
13	Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14	Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
18	Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.