Contains      

UniProtKB-P02749

Protein Beta-2-glycoprotein 1
Gene APOH
Status Reviewed
Source Bladder stromal cell line; Bladder tumor; Breast tumor; Cerebrospinal fluid; Colorectal tumor; HCC cell lines (Liver); HCCLM3 cell line (Liver); HepG2 cell line (Liver); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); Lung Adenocarcinoma; Lung tumor; Ovarian tumor; Pancreatic islets; Plasma; PlateletPBMC Macrophage cells; Prostate; Prostate cancer metastasis to liver; Prostate stromal cell line; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Serum (HCC); Urine; lung; ovarian tumor; plasma
Years 2005-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
162 AGnNSLYR 2014(33); 2015(unpublished);
162 SAGnNSLYR 2014(14); 2015(unpublished);
162 VYKPSAGnN 2014(33);
162 KPSAGnNSLY 2014(33);
162 PSAGnNSLYR 2009(66); 2011(56); 2012(48); 2012(53); 2013(38); 2013(42); 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
162 KPSAGnNSLYR 2012(48); 2014(14); 2014(33); 2015(unpublished);
162 VYKPSAGnNSL 2015(unpublished);
162 RVYKPSAGnNSL 2009(64);
162 VYKPSAGnNSLY 2012(48); 2014(33);
162 YKPSAGnNSLYR 2012(48); 2014(14); 2015(unpublished);
162 RVYKPSAGnNSLY 2014(33);
162 VYKPSAGnNSIYR 2014(32); 2014(32);
162 VYKPSAGnNSLYR 2005( 79); 2005(80); 2005(81); 2006(77); 2007(71); 2007(69); 2008(63); 2009(64); 2009(65); 2009(65); 2009(66); 2009(59); 2010(40); 2010(54); 2011(55); 2011(3); 2012(48); 2012(51); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(38); 2013(39); 2013(40); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(35); 2014(36); 2014(19); 2014(21); 2014(22); 2014(26); 2014(28); 2014(31); 2014(33); 2014(5); 2015(7); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;2007;2007;2007;
162 RVYKPSAGnNSLYR 2015(unpublished);
162 VYKPSAGnNSLYRD 2015(unpublished);
162 PSAGnNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTK 2013(38); 2013(38);
162 VYKPSAGnNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTK 2013(38); 2015(unpublished);
183 GnDTITCTTHGNW 2014(33);
183 GnDTITCTTHGNWTK 2012(3); 2014(33);
183 ECLPQHAMFGnDTITC 2014(33);
183 FGnDTITCTTHGNWTK 2014(33); 2015(unpublished);
183 AMFGnDTITCTTHGNWTK 2014(33); 2015(unpublished);
183 HAMFGnDTITCTTHGNWTK 2012(48); 2014(33); 2015(unpublished);
183 CLPQHAMFGnDTITCTTHGNW 2014(33);
183 ECLPQHAMFGnDTITCTTHGNW 2014(33);
183 CLPQHAMFGnDTITCTTHGNWTK 2014(33);
183 DTAVFECLPQHAMFGnDTITCTTH 2015(unpublished);
183 ECLPQHAMFGnDTITCTTHGNWTK 2014(33);
183 FECLPQHAMFGnDTITCTTHGNWTK 2012(48); 2014(28);
183 CLPQHAMFGnDTITCTTHGNWTKLPE 2013(42); 2014(33);
183 DTAVFECLPQHAMFGnDTITCTTHGN 2015(unpublished);
183 DTAVFECIPQHAMFGnDTITCTTHGNWTK 2014(32);
183 DTAVFECLPQHAMFGnDTITCTTHGNWTK 2005( 81); 2007(69); 2008(64); 2009(48); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(40); 2013(45); 2013(13); 2014(14); 2014(35); 2014(20); 2014(24); 2014(28); 2014(33); 2014(5); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;2007;20072007 ;
183 DTAVFECLPQHAMFGnDTITCTTHGNWTKLPECR 2012(53); 2013(38); 2014(35); 2015(unpublished);
183 PSAGNNSLYRDTAVFECLPQHAMFGnDTITCTTHGNWTK 2013(38); 2013(38);
183 VYKPSAGNNSLYRDTAVFECLPQHAMFGnDTITCTTHGNWTK 2013(38); 2015(unpublished);
193 TTHGnWTK 2015(unpublished);
193 CTTHGnWTK 2014(33);
193 THGnWTKLPE 2014(33);
193 GNDTITCTTHGnW 2014(33);
193 GNDTITCTTHGnWTK 2012(3); 2014(33);
193 TITCTTHGnWTKLPE 2013(42);
193 FGNDTITCTTHGnWTK 2014(33); 2015(unpublished);
193 AMFGNDTITCTTHGnWTK 2014(33); 2015(unpublished);
193 HAMFGNDTITCTTHGnWTK 2012(48); 2014(33); 2015(unpublished);
193 CLPQHAMFGNDTITCTTHGnW 2014(33);
193 ECLPQHAMFGNDTITCTTHGnW 2014(33);
193 CLPQHAMFGNDTITCTTHGnWTK 2014(33);
193 ECLPQHAMFGNDTITCTTHGnWTK 2014(33);
193 FECLPQHAMFGNDTITCTTHGnWTK 2012(48); 2014(28);
193 CLPQHAMFGNDTITCTTHGnWTKLPE 2013(42); 2014(33);
193 DTAVFECIPQHAMFGNDTITCTTHGnWTK 2014(32);
193 DTAVFECLPQHAMFGNDTITCTTHGnWTK 2005( 81); 2007(69); 2008(64); 2009(48); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(40); 2013(45); 2013(13); 2014(14); 2014(35); 2014(20); 2014(24); 2014(28); 2014(33); 2014(5); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(UnpublishedUnpublished ); 2007;2007;2007;2007;2007;20072007 ;
193 DTAVFECLPQHAMFGNDTITCTTHGnWTKLPECR 2012(53); 2013(38); 2014(35); 2015(unpublished);
193 PSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGnWTK 2013(38); 2013(38);
193 VYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGnWTK 2013(38); 2015(unpublished);
253 IGnWSAMPSCK 2014(32); 2014(32);
253 LGnWSAMPSCK 2005( 79); 2005(80); 2005(81); 2006(77); 2007(69); 2008(63); 2009(64); 2009(65); 2009(65); 2009(40); 2010(54); 2011(3); 2012(48); 2012(49); 2012(51); 2012(53); 2012(34); 2013(38); 2013(38); 2013(38); 2013(38); 2013(39); 2013(40); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(36); 2014(19); 2014(21); 2014(22); 2014(24); 2014(26); 2014(28); 2014(31); 2014(33); 2014(7); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;2007;
253 LGnWSAMPSCKASCK 2014(24); 2015(unpublished);
253 SLDGPEEIECTKLGnW 2014(33);
253 SLDGPEEIECTKLGnWSAMPSCKA 2014(33);
253 SLDGPEEIECTKLGnWSAMPSCKASC 2014(33);
253 ATFGCHDGYSLDGPEEIECTKLGnWSAMPSCK 2012(53); 2013(38); 2013(38); 2013(38); 2013(38); 2014(24); 2015(unpublished);
253 DKATFGCHDGYSLDGPEEIECTKLGnWSAMPSCK 2013(38); 2013(38); 2015(unpublished);

Sequence

1112131415161718191
MISPVLILFSSFLCHVAIAGRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTF
101111121131141151161171181191
EYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPEC
201211221231241251261271281291
REVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFC
301311321331341
KNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPC

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
26Takakura D, Harazono A, Hashii N, Kawasaki N: Selective glycopeptide profiling by acetone enrichment and LC/MS. Journal of Proteomics 2014, 101:17-30.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
36Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW: Glycoproteomic analysis of bronchoalveolar lavage (BAL) fluid identifies tumor-associated glycoproteins from lung adenocarcinoma. Journal of proteome research 2013, 12:3689-3696.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
49Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
51Yeh C-H, Chen S-H, Li D-T, Lin H-P, Huang H-J, Chang C-I, Shih W-L, Chern C-L, Shi F-K, Hsu J-L: Magnetic bead-based hydrophilic interaction liquid chromatography for glycopeptide enrichments. Journal of Chromatography A 2012, 1224:70-78.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
59Lee H-J, Na K, Choi E-Y, Kim KS, Kim H, Paik Y-K: Simple Method for Quantitative Analysis of N-Linked Glycoproteins in Hepatocellular Carcinoma Specimens. Journal of Proteome Research 2010, 9:308-318.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
77Ramachandran P, Boontheung P, Xie YM, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. Journal of Proteome Research 2006, 5:1493-1503.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.