Contains      

UniProtKB-P04003

Protein C4b-binding protein alpha chain
Gene C4BPA
Status Reviewed
Source Breast tumor; Colorectal tumor; HuH-7 cell line (Liver); Liver; Liver tumor (HCC); Ovarian tumor; Plasma; Prostate; Prostate tumor; Serum; Serum (HCC); plasma
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
221 TVEnETIGVW 2014(33);
221 CTVEnETIGVW 2014(33);
221 SCTVEnETIGVW 2014(33);
221 FSLLGHASISCTVEnE 2014(33);
221 GHASISCTVEnETIGVW 2014(33);
221 SLLGHASISCTVEnETIGVW 2014(33);
221 FSLLGHASISCTVEnETIGVWR 2014(33); 2015(unpublished);2015(8);
221 ASISCTVEnETIGVWRPSPPTCEK 2007(71); 2010(60);
221 FSIIGHASISCTVEnETIGVWRPSPPTCEK 2014(32); 2014(32);
221 FSLLGHASISCTVEnETIGVWRPSPPTCEK 2005( 81); 2009(64); 2012(53); 2013(38); 2013(45); 2014(13); 2014(35); 2014(20); 2014(28); 2014(33); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007;2007;
506 nVTIQCD 2014(33);
506 VDKDQYVEPEnVT 2009(64);
506 LSVDKDQYVEPEnV 2014(14);
506 VEPEnVTIQCDSGY 2014(33);
506 SVDKDQYVEPEnVTIQ 2014(33);
506 LSVDKDQYVEPEnVTIQ 2012(3);
506 LSVDKDQYVEPEnVTIQCD 2014(33);
506 SVDKDQYVEPEnVTIQCDSGY 2014(33);
506 DQYVEPEnVTIQCDSGYGVVGPQSITCSGNR 2014(13); 2014(22); 2014(24); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
506 CRKPELVNGRLSVDKDQYVEPEnVTIQCDSGY 2014(33);
506 ISVDKDQYVEPEnVTIQCDSGYGVVGPQSITCSGNR 2014(32);
506 LSVDKDQYVEPEnVTIQCDSGYGVVGPQSITCSGNR 2005(81); 2010(40); 2012(53); 2013(34); 2013(38); 2013(38); 2013(38); 2013(45); 2014(13); 2014(35); 2014(20); 2014(33); 2015(unpublished);2015(unpublished);
506 DQYVEPEnVTIQCDSGYGVVGPQSITCSGNRTWYPEVPK 2013(38);
506 LSVDKDQYVEPEnVTIQCDSGYGVVGPQSITCSGNRTWYPEVPK 2012(53);
528 SGnRTWYPEVPKCEW 2014(33);
528 SGYGVVGPQSITCSGnR 2014(33);
528 SITCSGnRTWYPEVPKCEW 2014(33);
528 SGYGVVGPQSITCSGnRTWYPE 2013(42);
528 GVVGPQSITCSGnRTWYPEVPKCEW 2014(33);
528 SGnRTWYPEVPKCEWETPEGCEQVL 2014(33);
528 SGYGVVGPQSITCSGnRTWYPEVPKCE 2013(42);
528 DQYVEPENVTIQCDSGYGVVGPQSITCSGnR 2014(13); 2014(22); 2014(24); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
528 ISVDKDQYVEPENVTIQCDSGYGVVGPQSITCSGnR 2014(32);
528 LSVDKDQYVEPENVTIQCDSGYGVVGPQSITCSGnR 2005(81); 2010(40); 2012(53); 2013(34); 2013(38); 2013(38); 2013(38); 2013(45); 2014(13); 2014(35); 2014(20); 2014(33); 2015(unpublished);2015(unpublished);
528 DQYVEPENVTIQCDSGYGVVGPQSITCSGnRTWYPEVPK 2013(38);
528 LSVDKDQYVEPENVTIQCDSGYGVVGPQSITCSGnRTWYPEVPK 2012(53);

Sequence

1112131415161718191
MHPPKTPSGALHRKRKMAAWPFSRLWKVSDPILFQMTLIAALLPAVLGNCGPPPTLSFAAPMDITLTETRFKTGTTLKYTCLPGYVRSHSTQTLTCNSDG
101111121131141151161171181191
EWVYNTFCIYKRCRHPGELRNGQVEIKTDLSFGSQIEFSCSEGFFLIGSTTSRCEVQDRGVGWSHPLPQCEIVKCKPPPDIRNGRHSGEENFYAYGFSVT
201211221231241251261271281291
YSCDPRFSLLGHASISCTVENETIGVWRPSPPTCEKITCRKPDVSHGEMVSGFGPIYNYKDTIVFKCQKGFVLRGSSVIHCDADSKWNPSPPACEPNSCI
301311321331341351361371381391
NLPDIPHASWETYPRPTKEDVYVVGTVLRYRCHPGYKPTTDEPTTVICQKNLRWTPYQGCEALCCPEPKLNNGEITQHRKSRPANHCVYFYGDEISFSCH
401411421431441451461471481491
ETSRFSAICQGDGTWSPRTPSCGDICNFPPKIAHGHYKQSSSYSFFKEEIIYECDKGYILVGQAKLSCSYSHWSAPAPQCKALCRKPELVNGRLSVDKDQ
501511521531541551561571581591
YVEPENVTIQCDSGYGVVGPQSITCSGNRTWYPEVPKCEWETPEGCEQVLTGKRLMQCLPNPEDVKMALEVYKLSLEIEQLELQRDSARQSTLDKEL

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.