Contains      

UniProtKB-P04004

Protein Vitronectin
Gene VTN
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Bladder tumor; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal tumor; HCC cell lines (Liver); HepG2 cell line (Liver); HuH-7 cell line (Liver); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Lung Adenocarcinoma; Lung tumor; Lymphocytes; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Plasma; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Saliva; Serum; Serum (Colorectal cancer); Serum (HCC); Urine; lung; ovarian tumor; plasma; plasmaJurkat T cell line
Years 2004-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
86 NnATVHE 2014(33);
86 KNnATVHE 2014(33);
86 NnATVHEQV 2014(33);
86 NnATVHEQVG 2015(unpublished);
86 DDGEEKNnATVH 2014(33);
86 NnATVHEQVGGP 2014(33);
86 VYDDGEEKNnAT 2009(64);
86 DDGEEKNnATVHE 2014(33);
86 NnATVHEQVGGPS 2014(14); 2014(33); 2015(unpublished);
86 NnATVHEQVGGPSL 2014(33);
86 YTVYDDGEEKNnAT 2014(33);
86 NnATVHEQVGGPSLT 2007(71); 2012(3); 2012(48);
86 EKNnATVHEQVGGPSL 2009(64);
86 YTVYDDGEEKNnATVH 2014(33);
86 NnATVHEQVGGPSLTSD 2014(14); 2015(unpublished);
86 YTVYDDGEEKNnATVHE 2009(64); 2014(33);
86 NnATVHEQVGGPSLTSDLQ 2014(33); 2015(unpublished);
86 NnATVHEQVGGPSLTSDLQA 2014(27);
86 NnATVHEQVGGPSLTSDLQAQ 2014(27); 2014(33); 2015(unpublished);
86 nATVHEQVGGPSLTSDLQAQSK 2011(56); 2014(27); 2014(33); 2015(unpublished);
86 NnATVHEQVGGPSLTSDLQAQS 2007(71);
86 NnATVHEQVGGPSITSDIQAQSK 2014(32); 2014(32);
86 NnATVHEQVGGPSLTSDLQAQSK 2005( 81); 2007(71); 2007(73); 2007(69); 2008(64); 2009(66); 2009(59); 2010(60); 2010(40); 2010(54); 2011(48); 2012(49); 2012(53); 2012(38); 2013(38); 2013(38); 2013(38); 2013(39); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(35); 2014(36); 2014(21); 2014(22); 2014(24); 2014(27); 2014(28); 2014(33); 2014(5); 2015(7); 2015(10); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;2007;
86 DDGEEKNnATVHEQVGGPSLTSDL 2014(33);
86 DDGEEKNnATVHEQVGGPSLTSDLQAQSK 2014(33);
86 GDVFTMPEDEYTVYDDGEEKNnATVHEQVGGPSLTSDLQAQSK 2014(33); 2015(unpublished);
169 nGSLFAFR 2004( 82); 2007(71); 2007(73); 2007(59); 2010(40); 2010(56); 2011(48); 2012(50); 2012(52); 2012(53); 2012(40); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(22); 2014(23); 2014(24); 2014(33); 2014(1); 2015(2); 2015(7); 2015(11); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2015;2015;2007;2007;2007;2007;2007;2007;2007 ;
169 FTDLKnGSL 2009(64);
169 KnGSLFAFR 2015(unpublished);
169 TDLKnGSLF 2014(33);
169 AFTDLKnGSLF 2014(33);
169 DAFTDLKnGSL 2014(33);
169 DAFTDLKnGSLF 2014(33);
169 FDAFTDLKnGSLF 2014(33);
169 DAFTDLKnGSLFAF 2014(33);
169 PFDAFTDLKnGSLF 2012(48); 2014(33);
169 FDAFTDLKnGSLFAFR 2014(33);
169 GKPFDAFTDLKnGSLF 2014(33);
169 PFDAFTDLKnGSLFAFR 2005(81); 2012(48); 2012(53); 2013(42); 2014(33);
169 SGKPFDAFTDLKnGSLF 2014(33);
169 LCSGKPFDAFTDLKnGSLF 2014(33);
169 SGKPFDAFTDLKnGSLFAFR 2014(33);
169 EELCSGKPFDAFTDLKnGSLF 2014(33);
169 EEELCSGKPFDAFTDLKnGSLF 2014(33);
169 LCSGKPFDAFTDLKnGSLFAFR 2014(33);
169 AEEELCSGKPFDAFTDLKnGSLF 2014(33);
169 QPPAEEELCSGKPFDAFTDLKnGSLF 2014(33);
169 TLHPGRPQPPAEEELCSGKPFDAFTDLKnGSLFAFR 2014(33);
242 DPDYPRnISDGF 2009(64);
242 VLDPDYPRnISDG 2009(64);
242 DGVLDPDYPRnISD 2013(42);
242 RFEDGVLDPDYPRnI 2014(33);
242 RFEDGVLDPDYPRnIS 2014(33);
242 EDGVLDPDYPRnISDGF 2009(64);
242 nISDGFDGIPDNVDAAL 2014(33);
242 nISDGFDGIPDNVDAALA 2014(33);
242 RFEDGVLDPDYPRnISDGF 2014(33);
242 nISDGFDGIPDNVDAALALPAH 2014(33); 2015(unpublished);
242 nISDGFDGIPDNVDAALALPAHS 2014(33);
242 nISDGFDGIPDNVDAALALPAHSY 2014(27); 2014(28); 2014(33); 2015(unpublished);
242 nISDGFDGIPDNVDAALALPAHSYSGR 2004( 82); 2005(79); 2005(80); 2005(81); 2007(71); 2007(66); 2009(59); 2010(40); 2010(56); 2011(53); 2012(34); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(35); 2014(22); 2014(33); 2014(5); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(UnpublishedUnpublished ); 2015;2007;2007;2007;2007;2007;2007;20072007 ;
242 RnISDGFDGIPDNVDAALALPAHSYSGR 2015(unpublished);
242 DPDYPRnISDGFDGIPDNVDAALALPAHSY 2014(33);
242 RFEDGVLDPDYPRnISDGFDGIPDNVDAAL 2014(33);
242 RFEDGVLDPDYPRnISDGFDGIPDNVDAALA 2014(33);
242 RFEDGVLDPDYPRnISDGFDGIPDNVDAALALPAH 2014(33);
242 RFEDGVLDPDYPRnISDGFDGIPDNVDAALALPAHSY 2014(33);
242 FEDGVLDPDYPRnISDGFDGIPDNVDAALALPAHSYSGR 2010(59); 2012(53); 2013(34); 2013(38); 2013(38); 2014(13); 2014(35); 2015(unpublished);2015(unpublished);2015(unpublished);
242 FEDGVLDPDYPRnISDGFDGIPDNVDAALALPAHSYSGRER 2013(38); 2015(unpublished);
242 GSQYWRFEDGVLDPDYPRnISDGFDGIPDNVDAALALPAHSYSGR 2013(38); 2013(38);

Sequence

1112131415161718191
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS
101111121131141151161171181191
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW
201211221231241251261271281291
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA
301311321331341351361371381391
VFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAT
401411421431441451461471
WLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
23Pan C, Zhou Y, Dator R, Ginghina C, Zhao Y, Movius J, Peskind E, Zabetian CP, Quinn J, Galasko D, et al: Targeted Discovery and Validation of Plasma Biomarkers of Parkinson's Disease. Journal of Proteome Research 2014, 13:4535-4545.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
27Wang J, Zhou C, Zhang W, Yao J, Lu HJ, Dong QZ, Zhou HJ, Qin LX: An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples. Proteome Science 2014, 12.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
36Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW: Glycoproteomic analysis of bronchoalveolar lavage (BAL) fluid identifies tumor-associated glycoproteins from lung adenocarcinoma. Journal of proteome research 2013, 12:3689-3696.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
49Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
59Lee H-J, Na K, Choi E-Y, Kim KS, Kim H, Paik Y-K: Simple Method for Quantitative Analysis of N-Linked Glycoproteins in Hepatocellular Carcinoma Specimens. Journal of Proteome Research 2010, 9:308-318.
60Liu Z, Cao L, He Y, Qiao L, Xu C, Lu H, Yang P: Tandem O-18 Stable Isotope Labeling for Quantification of N-Glycoproteome. Journal of Proteome Research 2010, 9:227-236.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
71Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen ON, Bunkenborg J: An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. Journal of Proteome Research 2007, 6:3021-3031.
73Larsen MR, Jensen SS, Jakobsen LA, Heegaard NHH: Exploring the sialiome using titanium dioxide chromatography and mass spectrometry. Molecular & Cellular Proteomics 2007, 6:1778-1787.
79Qiu RQ, Regnier FE: Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum. Analytical Chemistry 2005, 77:7225-7231.
80Qiu RQ, Regnier FE: Use of multidimensional lectin affinity chromatography in differential glycoproteomics. Analytical Chemistry 2005, 77:2802-2809.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.