Contains      

UniProtKB-P04216

Protein Thy-1 membrane glycoprotein
Gene THY1
Status Reviewed
Source Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); Hela cell line (cervical cancer); Liver; Liver tumor (HCC); Lymphocytes; Non-small Cell Lung Carcinoma; Ovarian tumor; Pancreatic islets; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Serum; Spermatozoa; Urine; XTC-1 Cell Line (Thyroid Cance)
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
42 HEnTSSSPIQY 2014(33);
42 HEnTSSSPIQYE 2014(33);
42 HEnTSSSPIQYEF 2012(48); 2014(14); 2014(33); 2015(unpublished);
42 DCRHEnTSSSPIQY 2014(33);
42 DCRHEnTSSSPIQYEF 2014(33);
42 LDCRHEnTSSSPIQYE 2014(33);
42 RLDCRHEnTSSSPIQY 2014(33);
42 HEnTSSSPIQYEFSITR 2013(43); 2014(32); 2014(32);
42 HEnTSSSPIQYEFSLTR 2007(62); 2009(62); 2009(62); 2009(48); 2012(52); 2012(34); 2013(45); 2013(14); 2014(18); 2014(22); 2014(33); 2014(5); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(Unpublished ); 2007;2007;2007;2007;2007;2007 ;
42 RHEnTSSSPIQYEFSLTR 2015(unpublished);
42 IDCRHEnTSSSPIQYEFSITR 2014(16);
42 LDCRHEnTSSSPIQYEFSLTR 2009(62); 2009(62); 2014(18); 2014(33); 2015(12);
79 SRTnF 2014(33);
79 TnFTSK 2014(22); 2015(unpublished);
79 TnFTSKY 2014(33);
79 SRTnFTSK 2013(34); 2014(16); 2014(33); 2015(unpublished);
79 SRTnFTSKY 2014(33);
119 SPPISSQnVTVLR 2014(33); 2015(unpublished);
119 GHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 SGHSPPISSQnVTVL 2014(33);
119 HSGHSPPISSQnVTVL 2014(33);
119 SGHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 HHSGHSPPISSQnVTVL 2014(33);
119 HSGHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 HHSGHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 LHHSGHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 ALHHSGHSPPISSQnVTVLR 2014(33); 2015(unpublished);
119 HSGHSPPISSQnVTVLRDKL 2014(33);
119 HHSGHSPPISSQnVTVLRDKL 2014(33);
119 TCALHHSGHSPPISSQnVTVLR 2014(33);
119 GTYTCALHHSGHSPPISSQnVTVLR 2012(48); 2014(33);
119 DEGTYTCAIHHSGHSPPISSQnVTVIR 2014(32); 2014(32);
119 DEGTYTCALHHSGHSPPISSQnVTVLR 2007(62); 2009(62); 2009(64); 2009(48); 2012(52); 2012(34); 2013(45); 2013(14); 2014(33); 2014(5); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(Unpublished ); 2007;2007;2007;2007;2007 ;
119 GTYTCALHHSGHSPPISSQnVTVLRDK 2014(33);
119 VIYISAFTSKDEGTYTCAIHHSGHSPPISSQnVTVIR 2014(16);
119 VLYLSAFTSKDEGTYTCALHHSGHSPPISSQnVTVLR 2013(45);

Sequence

1112131415161718191
MNLAISIALLLTVLQVSRGQKVTSLTACLVDQSLRLDCRHENTSSSPIQYEFSLTRETKKHVLFGTVGVPEHTYRSRTNFTSKYNMKVLYLSAFTSKDEG
101111121131141151161
TYTCALHHSGHSPPISSQNVTVLRDKLVKCEGISLLAQNTSWLLLLLLSLSLLQATDFMSL

Reference

ID Publication
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.