Contains      

UniProtKB-P04217

Protein Alpha-1B-glycoprotein
Gene A1BG
Status Reviewed
Source Bladder tumor; Breast tumor; Cerebrospinal fluid; HEK293 cell line (embryonic kidney); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); Lung tumor; Ovarian tumor; Plasma; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Serum; Serum (HCC); Urine; plasma
Years 2005-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
44 KPLAnVTL 2014(33);
44 KPLAnVTLT 2014(33);
44 LKPLAnVTL 2014(33);
44 LKPLAnVTLT 2014(33);
44 SLLKPLAnVTLTC 2014(33);
44 AESESLLKPLAnVTL 2014(33);
44 AESESLLKPLAnVTLT 2014(33);
44 ETQPSLWAESESLLKPLAnVTL 2014(33);
44 PSLWAESESLLKPLAnVTLTCQAHLETPDFQLFK 2007(unpublished);2007(unpublished);
179 PVHQPGnY 2014(33);
179 QPGnYSCSYR 2014(33);
179 ATFPVHQPGnY 2014(33);
179 HQPGnYSCSYR 2012(3);
179 ATFPVHQPGnYSC 2014(33);
179 DVEATFPVHQPGnY 2014(33);
179 ATFPVHQPGnYSCSY 2014(33);
179 EDVEATFPVHQPGnY 2014(33);
179 ATFPVHQPGnYSCSYR 2014(33);
179 AQEDVEATFPVHQPGnY 2014(33);
179 ATFPVHQPGnYSCSYRTD 2013(42);
179 DVEATFPVHQPGnYSCSYR 2014(33);
179 ATFPVHQPGnYSCSYRTDGE 2013(42);
179 EDVEATFPVHQPGnYSCSYR 2014(14); 2014(33); 2015(unpublished);
179 EVPEAQEDVEATFPVHQPGnY 2014(33);
179 AQEDVEATFPVHQPGnYSCSYR 2014(33);
179 LEVPEAQEDVEATFPVHQPGnY 2012(49); 2014(33);
179 EVPEAQEDVEATFPVHQPGnYSCSYR 2014(33); 2015(unpublished);
179 EGDHEFLEVPEAQEDVEATFPVHQPGnY 2014(19); 2014(33); 2015(unpublished);
179 EGDHEFLEVPEAQEDVEATFPVHQPGnYS 2015(unpublished);
179 REGDHEFLEVPEAQEDVEATFPVHQPGnY 2014(33);
179 EGDHEFLEVPEAQEDVEATFPVHQPGnYSC 2014(33); 2015(unpublished);
179 RREGDHEFLEVPEAQEDVEATFPVHQPGnY 2014(33);
179 LRREGDHEFLEVPEAQEDVEATFPVHQPGnY 2014(33);
179 REGDHEFLEVPEAQEDVEATFPVHQPGnYSC 2015(unpublished);
179 LLRREGDHEFLEVPEAQEDVEATFPVHQPGnY 2014(33);
179 EGDHEFLEVPEAQEDVEATFPVHQPGnYSCSYR 2005( 81); 2007(69); 2008(59); 2010(40); 2010(53); 2012(34); 2013(38); 2013(38); 2013(42); 2013(45); 2013(45); 2013(13); 2014(14); 2014(35); 2014(15); 2014(20); 2014(24); 2014(31); 2014(33); 2014(5); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(UnpublishedUnpublished ); 2007;2007;2007;2007;2007;2007;20072007 ;
179 REGDHEFIEVPEAQEDVEATFPVHQPGnYSCSYR 2014(32); 2014(32);
179 REGDHEFLEVPEAQEDVEATFPVHQPGnYSCSYR 2010(40); 2012(53); 2013(38); 2013(38); 2013(42); 2013(45); 2014(13); 2014(35); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(11);
179 GVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGnYSCSYR 2013(38);
363 LHnISVAD 2013(42); 2014(33);
363 LHnISVADSANY 2014(33);
363 ELHnISVADSANY 2014(33);
363 FQSPAGTEALFELHnI 2014(14);
363 LHnISVADSANYSCVY 2014(33);
363 ELHnISVADSANYSCVY 2014(33);
363 FQSPAGTEALFELHnIS 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007 (Unpublished );
363 LHnISVADSANYSCVYVD 2013(42);
363 FQSPAGTEALFELHnISVA 2014(28);
363 FQSPAGTEALFELHnISVAD 2007(unpublished);
363 FQSPAGTEALFELHnISVADSANYSCVY 2014(33);
363 LHnISVADSANYSCVYVDLKPPFGGSAPSER 2014(33);
363 FQSPAGTEALFELHnISVADSANYSCVYVDLKPPFGGSAPSER 2005(81); 2013(38); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(20); 2015(unpublished);2015(unpublished);
371 SAnYSCVYVD 2013(42); 2014(33);
371 LHNISVADSAnY 2014(33);
371 ELHNISVADSAnY 2014(33);
371 LHNISVADSAnYSCVY 2014(33);
371 ELHNISVADSAnYSCVY 2014(33);
371 LHNISVADSAnYSCVYVD 2013(42);
371 nYSCVYVDLKPPFGGSAPSER 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007 (Unpublished );
371 SAnYSCVYVDLKPPFGGSAPSER 2007(unpublished);2014(33);
371 FQSPAGTEALFELHNISVADSAnYSCVY 2014(33);
371 LHNISVADSAnYSCVYVDLKPPFGGSAPSER 2014(33);
371 FQSPAGTEALFELHNISVADSAnYSCVYVDLKPPFGGSAPSER 2005(81); 2013(38); 2013(42); 2013(45); 2014(13); 2014(35); 2014(15); 2014(20); 2015(unpublished);2015(unpublished);

Sequence

1112131415161718191
MSMLVVFLLLWGVTWGPVTEAAIFYETQPSLWAESESLLKPLANVTLTCQAHLETPDFQLFKNGVAQEPVHLDSPAIKHQFLLTGDTQGRYRCRSGLSTG
101111121131141151161171181191
WTQLSKLLELTGPKSLPAPWLSMAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVT
201211221231241251261271281291
IEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELI
301311321331341351361371381391
LSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPP
401411421431441451461471481491
PRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
11Wang M, Zhang X, Deng C: Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15:2158-2165.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
20Kim JY, Oh D, Kim S-K, Kang D, Moon MH: Isotope-Coded Carbamidomethylation for Quantification of N-Glycoproteins with Online Microbore Hollow Fiber Enzyme Reactor-Nanoflow Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2014, 86:7650-7657.
24Song E, Zhu R, Hammond ZT, Mechref Y: LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography. Journal of Proteome Research 2014, 13:4808-4820.
28Wang Y, Liu M, Xie L, Fang C, Xiong H, Lu H: Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry 2014, 86:2057-2064.
31Zhang L, Jiang H, Yao J, Wang Y, Fang C, Yang P, Lu H: Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers. Chemical Communications 2014, 50:1027-1029.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
49Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
59Lee H-J, Na K, Choi E-Y, Kim KS, Kim H, Paik Y-K: Simple Method for Quantitative Analysis of N-Linked Glycoproteins in Hepatocellular Carcinoma Specimens. Journal of Proteome Research 2010, 9:308-318.
69Calvano CD, Zambonin CG, Jensen ON: Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry. Journal of Proteomics 2008, 71:304-317.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.