Contains      

UniProtKB-P04626

Protein Receptor tyrosine-protein kinase erbB-2
Gene ERBB2
Status Reviewed
Source Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); HepG2 cell line (Liver); HuH-7 cell line (Liver); LNCap/PC3 cell lines (Prostate cancer); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Urine
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
68 LTYLPTnASLSFLQDIQE 2014(33);
68 HLYQGCQVVQGNLELTYLPTnASLS 2007(unpublished);2007(unpublished);2007(unpublished);
124 nNTTPVTGASPGGLR 2015(1);
124 DNYALAVLDNGDPLnNTTPVTGASPGGLR 2014(33);
124 GTQLFEDNYALAVLDNGDPLnNTTPVTGASPGGLR 2012(52); 2015(1); 2015(2); 2015(12); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
125 nTTPVTGASPGGLR 2007(unpublished);
125 NnTTPVTGASPGGLR 2015(1);
125 DNYALAVLDNGDPLNnTTPVTGASPGGLR 2014(33);
125 GTQLFEDNYALAVLDNGDPLNnTTPVTGASPGGLR 2012(52); 2015(1); 2015(2); 2015(12); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
187 ITIIDTnRSRACH 2013(37);
187 NNQLALTLIDTnR 2012(52); 2013(34); 2013(34); 2014(33); 2015(unpublished);2015(unpublished);2015;2007;2007;
259 HFnHSGICEL 2014(33);
259 IACIHFnHSGICE 2013(37);
259 HSDCLACLHFnHSGI 2007(unpublished);2007(unpublished);2007(unpublished);
259 HSDCLACLHFnHSGICE 2014(33);
259 HSDCLACLHFnHSGICELHCPALVTYNTDTFESMPNPEGR 2012(52);
530 nCSQFLR 2015(1);
530 GPTQCVnCSQFIR 2013(37);
530 GHCWGPGPTQCVnCSQFLR 2012(52); 2013(34); 2013(34); 2014(15); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;
571 PECQPQnGSVTCF 2013(37);
571 HCLPCHPECQPQnGSVT 2007(unpublished);2007(unpublished);2007(unpublished);
571 nGSVTCFGPEADQCVACAHYK 2015(1);
571 HCLPCHPECQPQnGSVTCFGPEADQCVACAHYK 2012(52); 2013(38); 2013(38); 2014(33);
571 HCLPCHPECQPQnGSVTCFGPEADQCVACAHYKDPPFCVAR 2011(58); 2012(52);
629 CQPCPInCTHSCV 2013(37);
629 GACQPCPInCTHSCVDLDDK 2014(33);
629 FPDEEGACQPCPInCTHSCVDLDDK 2012(52); 2013(34); 2014(22); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
629 GACQPCPInCTHSCVDLDDKGCPAEQR 2014(33);
629 FPDEEGACQPCPInCTHSCVDLDDKGCPAEQR 2012(52); 2013(34); 2014(15);

Sequence

1112131415161718191
MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLR
101111121131141151161171181191
IVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCK
201211221231241251261271281291
GSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACP
301311321331341351361371381391
YNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVF
401411421431441451461471481491
ETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRP
501511521531541551561571581591
EDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARC
601611621631641651661671681691
PSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPL
701711721731741751761771781791
TPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQL
801811821831841851861871881891
MPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFT
901911921931941951961971981991
HQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPL
1001101110211031104110511061107110811091
DSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQS
1101111111211131114111511161117111811191
LPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQ
120112111221123112411251
GGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.