| Protein | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
| Gene | RPN1 |
| Status | Reviewed |
| Source | Breast cancer xenografts; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); HEK293T cell line (embryonic kidney); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Platelet; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Spermatozoa; Urine; ovarian tumor |
| Years | 2007-2015 |
| Site | Identified Peptides | Year(Publication ID) |
|---|---|---|
| 220 | nNSPFLTITSMTR | 2015(1); |
| 220 | VHYEnNSPFLTITSMTR | 2012(52); |
| 299 | IGnVSTSH | 2014(33); |
| 299 | IGnVSTSHL | 2014(33); |
| 299 | YRDEIGnVS | 2014(33); |
| 299 | DEIGnVSTSH | 2011(54); 2012(48); 2014(33); 2015(unpublished); |
| 299 | IGnVSTSHLL | 2014(33); |
| 299 | RDEIGnVSTS | 2014(33); |
| 299 | DEIGnVSTSHL | 2014(33); |
| 299 | EIGnVSTSHLL | 2014(33); |
| 299 | RDEIGnVSTSH | 2014(33); |
| 299 | YRDEIGnVSTS | 2014(33); |
| 299 | DEIGnVSTSHLL | 2012(48); 2014(33); |
| 299 | IGnVSTSHLLIL | 2014(33); |
| 299 | RDEIGnVSTSHL | 2009(64); 2014(33); |
| 299 | YRDEIGnVSTSH | 2014(33); |
| 299 | IGnVSTSHLLILD | 2014(33); |
| 299 | RDEIGnVSTSHLL | 2014(33); |
| 299 | YRDEIGnVSTSHL | 2009(64); 2014(33); |
| 299 | DEIGnVSTSHLLIL | 2014(33); |
| 299 | IGnVSTSHLLILDD | 2014(33); |
| 299 | VYYRDEIGnVSTSH | 2009(64); |
| 299 | YRDEIGnVSTSHLL | 2014(33); |
| 299 | YYRDEIGnVSTSHL | 2014(33); |
| 299 | DEIGnVSTSHLLILD | 2012(48); 2014(33); |
| 299 | YRDEIGnVSTSHLLI | 2014(33); |
| 299 | YYRDEIGnVSTSHLL | 2014(33); |
| 299 | DEIGnVSTSHLLILDD | 2014(33); |
| 299 | IGnVSTSHLLILDDSVE | 2014(33); |
| 299 | IGnVSTSHLLILDDSVEM | 2014(33); |
| 299 | AAQDVYYRDEIGnVSTSHL | 2014(33); |
| 299 | DEIGnVSTSHLLILDDSVE | 2014(33); |
| 299 | IGnVSTSHLLILDDSVEME | 2014(33); |
| 299 | AAQDVYYRDEIGnVSTSHLL | 2014(33); |
| 299 | DEIGnVSTSHLLILDDSVEME | 2014(33); |
| 299 | nVSTSHLLILDDSVEMEIRPR | 2014(19); |
| 299 | GnVSTSHLLILDDSVEMEIRPR | 2012(48); |
| 299 | RDEIGnVSTSHLLILDDSVEME | 2014(33); |
| 299 | DEIGnVSTSHLLILDDSVEMEIR | 2009(64); 2012(48); 2014(33); 2015(unpublished); |
| 299 | IGnVSTSHLLILDDSVEMEIRPR | 2012(48); 2014(33); |
| 299 | TILPAAAQDVYYRDEIGnVSTSH | 2015(unpublished); |
| 299 | TILPAAAQDVYYRDEIGnVSTSHL | 2014(33); |
| 299 | DEIGnVSTSHIIIIDDSVEMEIRPR | 2014(32); 2014(32); |
| 299 | DEIGnVSTSHLLILDDSVEMEIRPR | 2007(75); 2007(64); 2009(57); 2011(48); 2012(52); 2012(34); 2013(34); 2013(34); 2013(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007; |
| 299 | KTILPAAAQDVYYRDEIGnVSTSHL | 2014(33); |
| 299 | KTILPAAAQDVYYRDEIGnVSTSHLL | 2014(33); |
| 299 | RDEIGnVSTSHLLILDDSVEMEIRPR | 2014(33); |
| 299 | YRDEIGnVSTSHLLILDDSVEMEIRPR | 2014(33); |
| 299 | TILPAAAQDVYYRDEIGnVSTSHLLILDDSVEMEIR | 2015(unpublished); |
| 299 | ILPAAAQDVYYRDEIGnVSTSHLLILDDSVEMEIRPR | 2015(unpublished); |
| 299 | TIIPAAAQDVYYRDEIGnVSTSHIIIIDDSVEMEIRPR | 2013(43); 2014(16); 2014(32); |
| 299 | TILPAAAQDVYYRDEIGnVSTSHLLILDDSVEMEIRPR | 2011(58); 2012(52); 2015(unpublished);2015(1); 2015(unpublished);2015(2); |
| 299 | DYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGnVSTSHLL | 2014(33); |
| 1 | 11 | 21 | 31 | 41 | 51 | 61 | 71 | 81 | 91 |
| MEAPAAGLFL | LLLLGTWAPA | PGSASSEAPP | LINEDVKRTV | DLSSHLAKVT | AEVVLAHLGG | GSTSRATSFL | LALEPELEAR | LAHLGVQVKG | EDEEENNLEV |
| 101 | 111 | 121 | 131 | 141 | 151 | 161 | 171 | 181 | 191 |
| RETKIKGKSG | RFFTVKLPVA | LDPGAKISVI | VETVYTHVLH | PYPTQITQSE | KQFVVFEGNH | YFYSPYPTKT | QTMRVKLASR | NVESYTKLGN | PTRSEDLLDY |
| 201 | 211 | 221 | 231 | 241 | 251 | 261 | 271 | 281 | 291 |
| GPFRDVPAYS | QDTFKVHYEN | NSPFLTITSM | TRVIEVSHWG | NIAVEENVDL | KHTGAVLKGP | FSRYDYQRQP | DSGISSIRSF | KTILPAAAQD | VYYRDEIGNV |
| 301 | 311 | 321 | 331 | 341 | 351 | 361 | 371 | 381 | 391 |
| STSHLLILDD | SVEMEIRPRF | PLFGGWKTHY | IVGYNLPSYE | YLYNLGDQYA | LKMRFVDHVF | DEQVIDSLTV | KIILPEGAKN | IEIDSPYEIS | RAPDELHYTY |
| 401 | 411 | 421 | 431 | 441 | 451 | 461 | 471 | 481 | 491 |
| LDTFGRPVIV | AYKKNLVEQH | IQDIVVHYTF | NKVLMLQEPL | LVVAAFYILF | FTVIIYVRLD | FSITKDPAAE | ARMKVACITE | QVLTLVNKRI | GLYRHFDETV |
| 501 | 511 | 521 | 531 | 541 | 551 | 561 | 571 | 581 | 591 |
| NRYKQSRDIS | TLNSGKKSLE | TEHKALTSEI | ALLQSRLKTE | GSDLCDRVSE | MQKLDAQVKE | LVLKSAVEAE | RLVAGKLKKD | TYIENEKLIS | GKRQELVTKI |
| 601 | |||||||||
| DHILDAL |
| ID | Publication |
|---|---|
| 1 | Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication. |
| 2 | Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763. |
| 16 | Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251. |
| 19 | Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206. |
| 32 | Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837. |
| 33 | Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721. |
| 34 | Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615. |
| 43 | Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759. |
| 48 | Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608. |
| 52 | Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667. |
| 54 | Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687. |
| 57 | Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75. |
| 58 | Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993. |
| 64 | Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661. |
| 75 | Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149. |