Contains      

UniProtKB-P05186

Protein Alkaline phosphatase, tissue-nonspecific isozyme
Gene ALPL
Status Reviewed
Source Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Plasma; Prostate; Prostate tumor; Serum; Spermatozoa; Urine
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
140 nTTQGNEVTSILR 2015(unpublished);2015(1);
140 TERSRCnTTQGNE 2013(37);
140 CnTTQGNEVTSILR 2014(17); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
140 SRCnTTQGNEVTSIIR 2013(43); 2014(16);
140 SRCnTTQGNEVTSILR 2012(52); 2014(15); 2014(33); 2015(8); 2015(12);
230 nKTDVEYE 2014(33);
230 nKTDVEYESDEK 2013(34); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);
230 KYMYPKnKTDVEY 2013(37);
230 nKTDVEYESDEKAR 2014(17); 2014(33);
230 YMYPKnKTDVEYESDEK 2014(16);
271 HSHFIWnR 2015(unpublished);
271 IWnRTELL 2014(33);
271 YKHSHFIWnRTE 2014(33);
303 LNRNnVTDPSLSE 2014(33);
303 NnVTDPSISEMVVVAIQIIR 2013(43); 2014(16);
303 NnVTDPSLSEMVVVAIQILR 2014(33); 2015(unpublished);
430 VVGGEREnVSMV 2014(14); 2015(unpublished);
430 EnVSMVDYAHNNY 2014(33);
430 VVGGEREnVSMVDY 2014(33);
430 KVVGGEREnVSMVDY 2014(33);
430 VVGGEREnVSMVDYAH 2015(unpublished);
430 VVGGEREnVSMVDYAHNNY 2014(33);
430 nVSMVDYAHNNYQAQSAVPLR 2015(1);
430 EnVSMVDYAHNNYQAQSAVPIR 2014(16);
430 EnVSMVDYAHNNYQAQSAVPLR 2007(52); 2012(22); 2014(33); 2014(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
430 EnVSMVDYAHNNYQAQSAVPLRHE 2014(33);
430 VGGEREnVSMVDYAHNNYQAQSAVPLR 2015(unpublished);
430 VVGGEREnVSMVDYAHNNYQAQSAVPIR 2014(16);
430 VVGGEREnVSMVDYAHNNYQAQSAVPLR 2009(64); 2012(52); 2013(34); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(12);

Sequence

1112131415161718191
MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKT
101111121131141151161171181191
YNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQG
201211221231241251261271281291
CKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELN
301311321331341351361371381391
RNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAP
401411421431441451461471481491
MLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPA
501511521
SSAGSLAAGPLLLALALYPLSVLF

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.