Contains      

UniProtKB-P06276

Protein Cholinesterase
Gene BCHE
Status Reviewed
Source Breast Cancer cell lines; Breast cancer xenografts; Breast tumor; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; Lung Adenocarcinoma; Non-small Cell Lung Carcinoma; Ovarian tumor; Plasma; PlasmaJurkat T cell line; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Serum (Colorectal cancer); Serum (HCC); Urine; XTC-1 Cell Line (Thyroid Cance); lung; plasma
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
45 GMnLTVFGGTVTAF 2005(81); 2014(33);
45 GMnLTVFGGTVTAFLGIPYAQPPLGR 2012(53); 2014(35); 2015(unpublished);2007(unpublished);2007;
45 VRGMnLTVFGGTVTAFLGIPYAQPPLGR 2015(unpublished);
85 SDIWnATKY 2014(33);
85 WSDIWnATK 2005( 81); 2009(62); 2010(40); 2012(53); 2013(38); 2013(38); 2013(40); 2013(42); 2013(45); 2014(13); 2014(13); 2014(16); 2014(18); 2014(22); 2014(33); 2015(2); 2015(8); 2015(10); 2015(unpublished);2007(unpublished);2007;
85 WSDIWnATKY 2014(33);
85 KKPQSLTKWSDIWnATKY 2014(33);
134 nATVLIW 2007(unpublished);
134 IPAPKPKnATVL 2014(33);
134 LNVWIPAPKPKnAT 2014(33);
134 NVWIPAPKPKnATVL 2009(64); 2014(33);
134 LNVWIPAPKPKnATVL 2014(33);
134 nATVLIWIYGGGFQTGTSSLHVYDGK 2015(unpublished);
269 EARnRTLNLAKL 2014(33);
284 EnETEIIK 2009(66); 2012(53); 2013(34); 2013(39); 2013(40); 2014(13); 2014(13); 2014(14); 2014(36); 2014(16); 2014(18); 2014(22); 2014(33); 2015(unpublished);2015(2); 2015(unpublished);2015(7); 2015(8); 2015(10);
284 EnETEIIKCLR 2014(33);
284 LTGCSREnETE 2009(64);
284 TGCSREnETEIIK 2014(33);
284 LTGCSREnETEIIK 2007(38); 2013(38); 2013(13); 2014(18); 2014(33); 2014(8); 2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;
284 TGCSREnETEIIKC 2014(33);
284 TGCSREnETEIIKCL 2014(33);
369 DnNSIITR 2005( 81); 2007(39); 2013(42); 2013(45); 2013(13); 2014(13); 2014(14); 2014(36); 2014(16); 2014(22); 2014(33); 2014(2); 2015(7); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2015;2007;2007;2007;
369 PGFSKDnNSIITR 2013(37);
369 GAPGFSKDnNSIITRKEF 2014(33);
483 DnYTKAEE 2014(33);
483 DnYTKAEEIISR 2014(16);
483 DnYTKAEEILSR 2005( 81); 2007(62); 2009(38); 2013(38); 2013(42); 2013(13); 2014(18); 2014(21); 2014(33); 2014(4); 2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;
483 ERRDnYTKAEEIL 2014(33);
483 RDnYTKAEEILSR 2014(33);
509 YGNPnETQNNSTSWPVFK 2005( 81); 2009(62); 2013(38); 2013(42); 2014(13); 2014(35); 2014(18); 2014(22); 2014(33); 2015(4); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
513 TQnNSTSWPVFK 2014(33);
513 TQnNSTSWPVFKSTE 2013(42);
513 YGNPNETQnNSTSWPVFK 2005( 81); 2009(62); 2013(38); 2013(42); 2014(13); 2014(35); 2014(18); 2014(22); 2014(33); 2015(4); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
514 TQNnSTSWPVFK 2014(33);
514 TQNnSTSWPVFKSTE 2013(42);
514 YGNPNETQNnSTSWPVFK 2005( 81); 2009(62); 2013(38); 2013(42); 2014(13); 2014(35); 2014(18); 2014(22); 2014(33); 2015(4); 2015(unpublished);2007(unpublished);2007(unpublished);2007;

Sequence

1112131415161718191
MHSKVTIICIRFLFWFLLLCMLIGKSHTEDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQS
101111121131141151161171181191
FPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQ
201211221231241251261271281291
LALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEI
301311321331341351361371381391
LLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFH
401411421431441451461471481491
YTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWA
501511521531541551561571581591
NFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCV
601
GL

Reference

ID Publication
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
4Cheow ESH, Sim KH, de Kleijn D, Lee CN, Sorokin V, Sze SK: Simultaneous Enrichment of Plasma Soluble and Extracellular Vesicular Glycoproteins Using Prolonged Ultracentrifugation-Electrostatic Repulsion-hydrophilic Interaction Chromatography (PUC-ERLIC) Approach. Molecular & Cellular Proteomics 2015, 14:1657-1671.
7Li Y, Shah P, De Marzo AM, Van Eyk JE, Lo Q, Chan DW, Zhang H: Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical Chemistry 2015, 87:4683-4687.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
21Liu L, Yu M, Zhang Y, Wang C, Lu H: Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides. Acs Applied Materials & Interfaces 2014, 6:7823-7832.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
36Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW: Glycoproteomic analysis of bronchoalveolar lavage (BAL) fluid identifies tumor-associated glycoproteins from lung adenocarcinoma. Journal of proteome research 2013, 12:3689-3696.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
39Li QK, Shah P, Li Y, Aiyetan PO, Chen J, Yung R, Molena D, Gabrielson E, Askin F, Chan DW, Zhang H: Glycoproteomic Analysis of Bronchoalveolar Lavage (BAL) Fluid Identifies Tumor-Associated Glycoproteins from Lung Adenocarcinonna. Journal of Proteome Research 2013, 12:3689-3696.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
66Jia W, Lu Z, Fu Y, Wang H-P, Wang L-H, Chi H, Yuan Z-F, Zheng Z-B, Song L-N, Han H-H, et al: A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins. Molecular & Cellular Proteomics 2009, 8:913-923.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.