Contains      

UniProtKB-P06756

Protein Integrin alpha-V
Gene ITGAV
Status Reviewed
Source 22Rv1 cell line (prostate cancer); 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HEK293 cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); Lymphocytes; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Ovarian tumorJurkat T cell line; Pancreatic islets; Plasma; Platelet; Platelet Plasma membranes; Prostate; Prostate cancer cell lines; Prostate tumor; SKOV-3 cell line (Ovarian cancer); SW1990 cell line (Pancreatic cancer); Urine; ovarian tumor; prostate cancer cell lines
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
74 AnTTQPGIVE 2014(33);
74 LVGAPKAnTTQPG 2009(64);
74 VGAPKAnTTQPGI 2013(37);
74 nTTQPGIVEGGQVLK 2015(1);
74 AnTTQPGIVEGGQVIK 2014(16); 2014(32); 2014(32);
74 AnTTQPGIVEGGQVLK 2007(72); 2007(74); 2007(75); 2007(62); 2009(64); 2009(67); 2009(54); 2011(47); 2012(48); 2012(50); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(13); 2014(14); 2014(15); 2014(17); 2014(18); 2014(22); 2014(23); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;
74 VGAPKAnTTQPGIVEGGQVL 2014(33);
74 LVGAPKAnTTQPGIVEGGQVL 2009(64); 2014(33);
74 LLVGAPKAnTTQPGIVEGGQVL 2014(33);
74 MFLLVGAPKAnTTQPGIVEGGQVLK 2011(58); 2012(52);
290 IYDGKnMSSLY 2014(33);
296 nFTGEQMAAY 2014(33);
296 nFTGEQMAAYF 2014(33);
488 VYPSILNQDnK 2014(33);
488 nKTCSLPGTALK 2015(1);
488 NQDnKTCSLPGTAL 2014(33);
488 ARPVITVNAGLEVYPSILNQDnK 2012(52);
488 ARPVITVNAGLEVYPSILNQDnKTCSLPGTALK 2012(52);
554 nMTISR 2013(40); 2014(33); 2015(unpublished);
554 SPSHSKnMTISR 2011(58); 2013(34); 2013(34); 2014(33); 2015(2); 2015(12);
615 TPAnISR 2014(33);
615 FTPAnISR 2015(unpublished);
615 QFTPAnISR 2014(33);
615 NQFTPAnISRQAHIL 2009(64);
615 GLQPILNQFTPAnISR 2014(33); 2015(unpublished);
615 NQFTPAnISRQAHILL 2009(64);
615 TAADTTGIQPIINQFTPAnISR 2014(16); 2014(32);
615 TAADTTGLQPILNQFTPAnISR 2003( 83); 2009(62); 2009(62); 2009(67); 2011(58); 2012(50); 2012(50); 2012(52); 2013(34); 2013(34); 2013(34); 2013(40); 2014(14); 2014(15); 2014(17); 2014(18); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;
704 AFKTEnQTRQ 2009(64);
704 ISCAFKTEnQTR 2014(16);
704 LSCAFKTEnQTR 2011(58); 2012(52); 2014(15); 2014(33); 2015(12);
835 KYNnNTLL 2014(33);
835 YNnNTLLY 2014(33);
835 YNnNTLLYILH 2012(48);
835 YNnNTLLYILHYDIDGPMNCTSDMEINPLR 2012(52); 2015(unpublished);2015(1);
835 YNnNTLLYILHYDIDGPMNCTSDMEINPLRIK 2013(38);
851 nCTSDMEINPLR 2015(1);
851 DIDGPMnCTSDMEINPL 2014(33);
851 ILHYDIDGPMnCTSDME 2014(33);
851 DIDGPMnCTSDMEINPLR 2007(unpublished);2007(unpublished);2007(unpublished);
851 HYDIDGPMnCTSDMEINPLR 2015(unpublished);
851 ILHYDIDGPMnCTSDMEINPL 2014(33);
851 ILHYDIDGPMnCTSDMEINPLR 2007(75); 2012(48); 2014(33); 2015(unpublished);
851 YNNNTLLYILHYDIDGPMnCTSDMEINPLR 2012(52); 2015(unpublished);2015(1);
851 YNNNTLLYILHYDIDGPMnCTSDMEINPLRIK 2013(38);
874 nDTVAGQGER 2012(52); 2014(22); 2015(1);
874 KnDTVAGQGER 2014(33);
874 IQTTEKnDTVAGQ 2013(37);
874 TTEKnDTVAGQGER 2012(48);
874 ISSLQTTEKnDTVAGQ 2007(75);
874 SLQTTEKnDTVAGQGER 2012(48); 2015(unpublished);
874 ISSLQTTEKnDTVAGQGE 2014(33);
874 QTTEKnDTVAGQGERDHL 2014(33);
874 SSLQTTEKnDTVAGQGER 2012(48);
874 ISSIQTTEKnDTVAGQGER 2014(16);
874 ISSLQTTEKnDTVAGQGER 2007(72); 2007(74); 2007(75); 2007(62); 2009(64); 2009(67); 2009(58); 2011(48); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(40); 2013(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(25); 2014(33); 2014(1); 2015(2); 2015(3); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;
874 QTTEKnDTVAGQGERDHLIT 2014(33);
874 IKISSLQTTEKnDTVAGQGER 2012(52); 2015(1);
874 ISSLQTTEKnDTVAGQGERDH 2007(75); 2012(48);
874 ISSLQTTEKnDTVAGQGERDHL 2007(75);
874 ISSLQTTEKnDTVAGQGERDHLIT 2007(75);
874 RIKISSLQTTEKnDTVAGQGERDHL 2014(33);
945 nHSYSLK 2015(1);
945 NQnHSYSLK 2012(48); 2014(33);
945 ENQnHSYSIK 2014(16);
945 ENQnHSYSLK 2007(75); 2011(58); 2012(48); 2013(34); 2013(34); 2013(34); 2013(40); 2014(22); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
945 MNKENQnHSY 2014(33);
945 MNKENQnHSYSIK 2013(37);
945 SLLWTETFMNKENQnHSYSLK 2011(58); 2012(52); 2014(33); 2015(1); 2015(2); 2015(12);
973 NLPIEDITnSTL 2007(unpublished);2007(unpublished);
973 KNLPIEDITnSTL 2014(33);
973 NLPIEDITnSTLVTT 2014(33);
973 NLPIEDITnSTLVTTN 2014(33);

Sequence

1112131415161718191
MAFPPRRRLRLGPRGLPLLLSGLLLPLCRAFNLDVDSPAEYSGPEGSYFGFAVDFFVPSASSRMFLLVGAPKANTTQPGIVEGGQVLKCDWSSTRRCQPI
101111121131141151161171181191
EFDATGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAPLYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSQDIDADGQGFCQGGFSIDFTKADRVL
201211221231241251261271281291
LGGPGSFYWQGQLISDQVAEIVSKYDPNVYSIKYNNQLATRTAQAIFDDSYLGYSVAVGDFNGDGIDDFVSGVPRAARTLGMVYIYDGKNMSSLYNFTGE
301311321331341351361371381391
QMAAYFGFSVAATDINGDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQRASGDFQTTKLNGFEVFARFGSAIAPLGDLDQDGFNDIAIAAPYGGEDKK
401411421431441451461471481491
GIVYIFNGRSTGLNAVPSQILEGQWAARSMPPSFGYSMKGATDIDKNGYPDLIVGAFGVDRAILYRARPVITVNAGLEVYPSILNQDNKTCSLPGTALKV
501511521531541551561571581591
SCFNVRFCLKADGKGVLPRKLNFQVELLLDKLKQKGAIRRALFLYSRSPSHSKNMTISRGGLMQCEELIAYLRDESEFRDKLTPITIFMEYRLDYRTAAD
601611621631641651661671681691
TTGLQPILNQFTPANISRQAHILLDCGEDNVCKPKLEVSVDSDQKKIYIGDDNPLTLIVKAQNQGEGAYEAELIVSIPLQADFIGVVRNNEALARLSCAF
701711721731741751761771781791
KTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSEMDTSVKFDLQIQSSNLFDKVSPVVSHKVDLAVLAAVEIRGVSSPDHVFLPIPNWEHKENPETEE
801811821831841851861871881891
DVGPVVQHIYELRNNGPSSFSKAMLHLQWPYKYNNNTLLYILHYDIDGPMNCTSDMEINPLRIKISSLQTTEKNDTVAGQGERDHLITKRDLALSEGDIH
901911921931941951961971981991
TLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLLWTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTWGIQPAPMPVPVWVIILA
10011011102110311041
VLAGLLLLAVLVFVMYRMGFFKRVRPPQEEQEREQLQPHENGEGNSET

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
23Pan C, Zhou Y, Dator R, Ginghina C, Zhao Y, Movius J, Peskind E, Zabetian CP, Quinn J, Galasko D, et al: Targeted Discovery and Validation of Plasma Biomarkers of Parkinson's Disease. Journal of Proteome Research 2014, 13:4535-4545.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
47Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen S-H, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K: Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Molecular & Cellular Proteomics 2012, 11:M112. 017558.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.