Contains      

UniProtKB-P07585

Protein Decorin
Gene DCN
Status Reviewed
Source Bladder stromal cell line; Bladder tumor; Breast Cancer cell lines; Breast tumor; Cerebrospinal fluid; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Platelet; Prostate; Prostate stromal cell line; Prostate tumor; Serum; ovarian tumor
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
211 IRIADTnIT 2014(33);
211 IADTnITSIPQ 2014(33);
211 IADTnITSIPQG 2014(33);
211 RIADTnITSIPQ 2014(33);
211 IRIADTnITSIPQ 2013(37); 2014(33);
211 IADTnITSIPQGLPPSLTE 2014(14); 2014(33); 2015(unpublished);
211 IADTnITSIPQGLPPSLTEL 2014(14); 2014(33); 2015(unpublished);
211 IADTnITSIPQGLPPSLTELH 2014(33);
211 IRIADTnITSIPQGLPPSLTEL 2014(33);
211 TnITSIPQGLPPSLTELHLDGNK 2014(33);
211 IADTnITSIPQGLPPSLTELHLDGN 2014(33);
211 IADTnITSIPQGIPPSITEIHIDGNK 2014(32);
211 IADTnITSIPQGLPPSLTELHLDGNK 2009(64); 2011(54); 2013(34); 2013(40); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(Unpublished ); 2007;2007;2007 ;
211 IADTnITSIPQGLPPSLTELHLDGNKI 2015(unpublished);
211 RIADTnITSIPQGLPPSLTELHLDGNK 2015(unpublished);
211 IADTnITSIPQGLPPSLTELHLDGNKISR 2014(33);
262 ISAVDnGS 2009(64);
262 nGSLANTPHLR 2015(1);
262 NSISAVDnGSLA 2014(33);
262 NSISAVDnGSLAN 2014(33);
262 SISAVDnGSIANT 2013(37);
262 VDnGSLANTPHLR 2014(33);
262 AVDnGSLANTPHLR 2014(33); 2015(unpublished);
262 SFNSISAVDnGSLA 2014(33);
262 SAVDnGSLANTPHLR 2012(3); 2014(33); 2015(unpublished);
262 SISAVDnGSLANTPH 2014(33);
262 ISAVDnGSLANTPHLR 2014(33);
262 NSISAVDnGSLANTPH 2014(33);
262 SISAVDnGSLANTPHLR 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
262 LGLSFNSISAVDnGSLAN 2014(33);
262 NSISAVDnGSLANTPHLR 2014(14); 2014(33); 2015(unpublished);
262 SFNSISAVDnGSLANTPH 2014(33);
262 SFNSISAVDnGSLANTPHL 2014(33);
262 SISAVDnGSLANTPHLREL 2014(33);
262 GLSFNSISAVDnGSLANTPH 2014(33);
262 NSISAVDnGSLANTPHLREL 2014(33);
262 SFNSISAVDnGSLANTPHLR 2014(33); 2015(unpublished);
262 LGLSFNSISAVDnGSLANTPH 2014(33); 2015(unpublished);
262 GLSFNSISAVDnGSLANTPHLR 2014(33);
262 SFNSISAVDnGSLANTPHLREL 2014(33);
262 IGISFNSISAVDnGSIANTPHIR 2014(16); 2014(32); 2014(32);
262 LGLSFNSISAVDnGSLANTPHLR 2009(64); 2009(65); 2009(65); 2013(34); 2014(14); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
262 GLSFNSISAVDnGSLANTPHLREL 2014(33);
262 LGLSFNSISAVDnGSLANTPHLRE 2014(33);
262 SFNSISAVDnGSLANTPHLRELHLDNNKL 2014(33);
262 GLNNLAKLGLSFNSISAVDnGSLANTPHLR 2014(33);
262 VDAASLKGLNNLAKLGLSFNSISAVDnGSLANTPHLR 2014(33);
303 LHNNnISVVGSS 2014(33);
303 HNNnISVVGSSDF 2014(33);
303 LHNNnISVVGSSD 2014(33);
303 LHNNnISVVGSSDF 2014(33);
303 nISVVGSSDFCPPGHNTK 2015(unpublished);
303 NnISVVGSSDFCPPGHNTK 2014(33); 2015(unpublished);
303 LHNNnISVVGSSDFCPPGHN 2014(33);
303 HNNnISVVGSSDFCPPGHNTK 2014(33);
303 LHNNnISVVGSSDFCPPGHNTK 2014(33); 2015(unpublished);
303 LHNNnISVVGSSDFCPPGHNTKK 2014(33);
303 YLHNNnISVVGSSDFCPPGHNTK 2015(unpublished);
303 VYLHNNnISVVGSSDFCPPGHNTK 2015(unpublished);
303 LHNNnISVVGSSDFCPPGHNTKKASY 2014(33);
303 YIQVVYLHNNnISVVGSSDFCPPGHN 2015(unpublished);
303 YIQVVYLHNNnISVVGSSDFCPPGHNTK 2014(33); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
303 HKYIQVVYLHNNnISVVGSSDFCPPGHNTK 2014(33);

Sequence

1112131415161718191
MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGD
101111121131141151161171181191
FKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMK
201211221231241251261271281291
KLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLH
301311321331341351
NNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.