Contains      

UniProtKB-P07602

Protein Prosaposin
Gene PSAP
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Bladder stromal cell line; Bladder tumor; Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HepG2 cell line (Liver); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; PBMC Macrophage cells; Pancreatic islets; Plasma; Platelet; Prostate; Prostate cancer cell lines; Prostate cancer metastasis to liver; Prostate stromal cell line; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Saliva; Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); plasma; umbilical vein Endothelial Cells
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
80 LKDnATEEE 2009(64);
80 DnATEEEILVY 2014(33);
80 GDMIKDnATEEEI 2013(37);
80 nATEEEILVYLEK 2007(75);
80 DnATEEEILVYLEK 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(64); 2009(67); 2009(56); 2011(57); 2011(48); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(38); 2013(38); 2013(40); 2013(45); 2013(14); 2014(19); 2014(22); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;
80 KDnATEEEILVYLEK 2015(unpublished);
80 DVVTAAGDMLKDnATE 2007(75); 2014(33);
80 IKDnATEEEIIVYIEK 2014(16);
80 LKDnATEEEILVYLEK 2015(unpublished);
80 DVVTAAGDMLKDnATEE 2014(33);
80 VVTAAGDMLKDnATEEE 2014(33);
80 DMLKDnATEEEILVYLEK 2007(75);
80 DVVTAAGDMLKDnATEEE 2014(33);
80 DVVTAAGDMLKDnATEEEI 2014(14); 2015(unpublished);
80 DVVTAAGDMLKDnATEEEILV 2014(14); 2015(unpublished);
80 DVVTAAGDMLKDnATEEEILVY 2014(33);
80 DVVTAAGDMIKDnATEEEIIVYIEK 2014(32); 2014(32);
80 DVVTAAGDMLKDnATEEEILVYLEK 2003(83); 2007(75); 2009(62); 2009(62); 2009(62); 2009(62); 2009(64); 2011(57); 2012(48); 2012(52); 2013(34); 2013(34); 2013(34); 2013(38); 2013(38); 2013(45); 2014(14); 2014(25); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
80 SLPCDICKDVVTAAGDMLKDnATEEE 2014(33);
80 SIPCDICKDVVTAAGDMIKDnATEEEIIVYIEK 2014(16);
80 SLPCDICKDVVTAAGDMLKDnATEEEILVYLEK 2012(52); 2015(12);
80 DVVTAAGDMLKDnATEEEILVYLEKTCDWLPKPNMSASCK 2014(18);
80 LQTVWNKPTVKSLPCDICKDVVTAAGDMLKDnATEEEILVYLEK 2014(33);
101 nMSASCK 2015(1);
101 TCDWLPKPnMSA 2014(33);
101 DWIPKPnMSASCK 2013(37);
101 LEKTCDWLPKPnMS 2009(64);
101 TCDWIPKPnMSASCK 2014(16);
101 TCDWLPKPnMSASCK 2009(62); 2009(62); 2009(62); 2009(63); 2009(67); 2012(52); 2013(34); 2013(34); 2013(34); 2013(38); 2013(38); 2013(40); 2013(45); 2014(13); 2014(15); 2014(17); 2014(18); 2014(22); 2014(25); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
101 DVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPnMSASCK 2014(18);
215 TnSTFVQALVE 2014(33);
215 DIQTAVRTnSTF 2014(33);
215 TnSTFVQALVEH 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
215 QTAVRTnSTFVQA 2013(37);
215 TnSTFVQALVEHVK 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(65); 2009(65); 2009(67); 2009(40); 2010(57); 2011(48); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(38); 2013(38); 2013(40); 2013(44); 2013(45); 2013(45); 2013(13); 2014(14); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(5); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007;2007;2007;2007;2007;2007;2007 ;
215 VTDIQTAVRTnSTF 2014(33);
215 RTnSTFVQALVEHVK 2015(unpublished);
215 TnSTFVQALVEHVKE 2014(33);
215 TnSTFVQALVEHVKEE 2014(33); 2015(unpublished);
215 TnSTFVQAIVEHVKEECDR 2014(32); 2014(32);
215 TnSTFVQALVEHVKEECDR 2009(62); 2009(62); 2009(62); 2009(67); 2012(51); 2012(52); 2013(34); 2013(34); 2013(34); 2013(45); 2014(15); 2014(18); 2014(25); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
215 VRTnSTFVQALVEHVKEECDR 2014(33);
215 TnSTFVQAIVEHVKEECDRIGPGMADICK 2014(16);
215 TnSTFVQALVEHVKEECDRLGPGMADICK 2014(33); 2015(12);
215 DNGDVCQDCIQMVTDIQTAVRTnSTFVQALVEHVK 2013(38);
332 LIDNnK 2014(22); 2014(33); 2015(unpublished);
332 LIDNnKTE 2014(33);
332 IDNnKTEKE 2014(33);
332 IIDNnKTEK 2014(16);
332 LIDNnKTEK 2007(75); 2009(64); 2012(48); 2013(34); 2013(34); 2013(34); 2013(38); 2013(40); 2013(44); 2013(45); 2014(19); 2014(22); 2014(33); 2015(1); 2015(2); 2015(10); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;2007;
332 LIDNnKTEKE 2009(64); 2014(33);
332 IDNnKTEKEIL 2009(64); 2014(33);
332 VTKLIDNnKTE 2014(33);
332 VTKLIDNnKTEK 2014(33);
332 EVTKLIDNnKTEK 2014(18); 2014(33);
332 KLIDNnKTEKEIL 2014(33);
332 TKIIDNnKTEKEI 2013(37);
332 VTKLIDNnKTEKE 2014(33);
332 IDNnKTEKEILDAF 2014(33);
332 IDNnKTEKEILDAFDKM 2014(33);
332 LIDNnKTEKEILDAFDK 2007(unpublished);2007(75); 2009(62); 2009(62); 2009(62); 2009(62); 2009(62); 2012(48); 2012(52); 2013(34); 2013(34); 2014(14); 2014(15); 2014(17); 2014(18); 2014(19); 2014(25); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
332 LVKEVTKLIDNnKTEKEIL 2014(33);
332 LIDNnKTEKEILDAFDKMCSK 2014(33);
332 LIDNnKTEKEILDAFDKMCSKLPK 2014(33);
426 NLEKnSTK 2012(3); 2012(48); 2013(34); 2013(34); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007;2007;2007;2007 ;
426 KnSTKQEIL 2014(33);
426 NLEKnSTKQE 2014(33);
426 LDRNLEKnSTK 2014(33);
426 LEKnSTKQEIL 2009(64);
426 LDRNLEKnSTKQ 2014(33);
426 nSTKQEILAALE 2014(33);
426 DRNIEKnSTKQEI 2013(37);
426 nSTKQEIIAAIEK 2014(16);
426 nSTKQEILAALEK 2007(unpublished);2007(75); 2009(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(64); 2009(67); 2011(58); 2012(52); 2013(34); 2013(34); 2013(34); 2013(45); 2014(13); 2014(14); 2014(25); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
426 DRNLEKnSTKQEIL 2014(33);
426 KnSTKQEILAALEK 2014(33);
426 LDRNLEKnSTKQEIL 2014(33);
426 LVGYLDRNLEKnSTK 2009(64); 2014(18); 2014(33); 2015(1); 2015(2);
426 NLEKnSTKQEILAALE 2014(33);
426 LVGYLDRNLEKnSTKQE 2014(33);
426 NLEKnSTKQEILAALEK 2003(83); 2007(75); 2009(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(64); 2009(65); 2009(65); 2009(67); 2011(58); 2012(48); 2012(52); 2013(34); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(15); 2014(17); 2014(18); 2014(19); 2014(25); 2014(25); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
426 VGYLDRNLEKnSTKQEIL 2014(33);
426 LVGYLDRNLEKnSTKQEILAALEK 2014(33);
426 nSTKQEILAALEKGCSFLPDPYQK 2014(33);
426 KLVGYLDRNLEKnSTKQEILAALEK 2014(33);
426 NLEKnSTKQEILAALEKGCSFLPDPYQK 2014(33);

Sequence

1112131415161718191
MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKP
101111121131141151161171181191
NMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQD
201211221231241251261271281291
CIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVE
301311321331341351361371381391
PIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHV
401411421431441451461471481491
TQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPS
501511521
YWCQNTETAAQCNAVEHCKRHVWN

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
5Goyallon A, Cholet S, Chapelle M, Junot C, Fenaille F: Evaluation of a combined glycomics and glycoproteomics approach for studying the major glycoproteins present in biofluids: Application to cerebrospinal fluid. Rapid Communications in Mass Spectrometry 2015, 29:461-473.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
51Yeh C-H, Chen S-H, Li D-T, Lin H-P, Huang H-J, Chang C-I, Shih W-L, Chern C-L, Shi F-K, Hsu J-L: Magnetic bead-based hydrophilic interaction liquid chromatography for glycopeptide enrichments. Journal of Chromatography A 2012, 1224:70-78.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.
83Zhang H, Li XJ, Martin DB, Aebersold R: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nature Biotechnology 2003, 21:660-666.