Contains      

UniProtKB-P07686

Protein Beta-hexosaminidase subunit beta
Gene HEXB
Status Reviewed
Source Bladder tumor; Breast Cancer cell lines; Breast cancer cell lines; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); HCCLM3 cell line (Liver); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; LnCap cell line (Prostate cancer); OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Prostate; Prostate cancer cell lines; Prostate cancer metastasis to liver; Prostate tumor; Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance)
Years 2003-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
84 ISHSPnSTAGPSCT 2009(64);
84 ISHSPnSTAGPSCTL 2014(33);
84 NFYISHSPnSTAGPSC 2014(33);
84 nSTAGPSCTLLEEAFR 2015(1);
84 YISHSPnSTAGPSCTL 2009(64); 2014(33);
84 YISHSPnSTAGPSCTLL 2009(64);
84 ISHSPnSTAGPSCTLLEE 2014(33);
84 ISHSPnSTAGPSCTLLEEAF 2014(33);
84 NFYISHSPnSTAGPSCTLLE 2014(33);
84 ISHSPnSTAGPSCTLLEEAFR 2007(unpublished);2012(48); 2014(33);
84 NFYISHSPnSTAGPSCTLLEE 2014(33);
84 YISHSPnSTAGPSCTLLEEAF 2014(33);
84 YISHSPnSTAGPSCTLLEEAFR 2012(48); 2014(33);
84 NFYISHSPnSTAGPSCTLLEEAFR 2014(33);
84 ENFYISHSPnSTAGPSCTLLEEAFR 2012(48);
84 NFYISHSPnSTAGPSCTLLEEAFRR 2014(33);
84 APENFYISHSPnSTAGPSCTLLEEAFR 2012(48);
84 LAPENFYISHSPnSTAGPSCTLLEEAFR 2012(48);
84 LLHLAPENFYISHSPnSTAGPSCTLLEEAFR 2012(52); 2015(unpublished);
84 MTPNLLHLAPENFYISHSPnSTAGPSCTLLEEAFR 2009(62); 2012(52); 2013(34); 2013(34); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
84 MTPNIIHIAPENFYISHSPnSTAGPSCTIIEEAFRR 2014(16);
84 MTPNLLHLAPENFYISHSPnSTAGPSCTLLEEAFRR 2009(62); 2012(52); 2014(33); 2015(2);
142 CDAFPnISSDE 2014(33);
142 QSECDAFPnISSDESY 2014(33);
190 SYGTFTInE 2014(33);
190 nESTIIDSPR 2015(1);
190 InESTIIDSPR 2014(14); 2015(unpublished);
190 TInESTIIDSPR 2012(52); 2014(14); 2014(33); 2015(unpublished);
190 TInESTIIDSPRF 2014(33);
190 TInESTIIDSPRFS 2014(33);
190 GTFTInESTIIDSPR 2014(33);
190 TInESTIIDSPRFSH 2014(33);
190 SQLVYQDSYGTFTInESTIIDSPR 2015(unpublished);
190 GLETFSQLVYQDSYGTFTInESTIIDSPR 2007(75); 2015(2); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(UnpublishedUnpublished ); 20072007 ;
327 GPINPTLnTTY 2014(33);
327 NPTLnTTYSFL 2014(33);
327 nTTYSFLTTFFK 2015(1);
327 LDSFGPINPTLnT 2014(14);
327 PINPTInTTYSFI 2013(37);
327 LDSFGPINPTLnTTY 2011(57); 2014(33);
327 PTLnTTYSFLTTFFK 2015(unpublished);
327 DSFGPINPTLnTTYSF 2009(64);
327 KLDSFGPINPTLnTTY 2014(33);
327 LDSFGPINPTLnTTYSFL 2011(57);
327 IDSFGPINPTInTTYSFITTFFK 2014(16);
327 LDSFGPINPTLnTTYSFLTTFFK 2003( 83); 2007(62); 2009(62); 2009(63); 2009(64); 2009(67); 2009(58); 2011(33); 2014(2); 2015(9); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(Unpublished ); 2007;2007 ;
327 KLDSFGPINPTLnTTYSFLTTFFK 2015(unpublished);
327 LDSFGPINPTLnTTYSFLTTFFKE 2014(33);
327 QNKLDSFGPINPTLnTTYSFLTTFFK 2014(33); 2015(2);

Sequence

1112131415161718191
MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRR
101111121131141151161171181191
YHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRF
201211221231241251261271281291
SHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGK
301311321331341351361371381391
GQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINK
401411421431441451461471481491
GSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTP
501511521531541551
RLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.