Detail


Protein	Bifunctional glutamate/proline--tRNA ligase
Gene	EPRS
Status	Reviewed
Source	Liver; OVCAR-3 cell line (Ovarian cancer)
Years	2014-2015

Site	Identified Peptides	Year(Publication ID)
726	nETSAPFK	2015(1);
811	TGQEYKPGNPPAEIGQnISSNSSASILESK	2014(33);
815	nSSASILESK	2015(1);
815	TGQEYKPGNPPAEIGQNISSnSSASILESK	2014(33);

Sequence

1	11	21	31	41	51	61	71	81	91
MATLSLTVNS	GDPPLGALLA	VEHVKDDVSI	SVEEGKENIL	HVSENVIFTD	VNSILRYLAR	VATTAGLYGS	NLMEHTEIDH	WLEFSATKLS	SCDSFTSTIN
101	111	121	131	141	151	161	171	181	191
ELNHCLSLRT	YLVGNSLSLA	DLCVWATLKG	NAAWQEQLKQ	KKAPVHVKRW	FGFLEAQQAF	QSVGTKWDVS	TTKARVAPEK	KQDVGKFVEL	PGAEMGKVTV
201	211	221	231	241	251	261	271	281	291
RFPPEASGYL	HIGHAKAALL	NQHYQVNFKG	KLIMRFDDTN	PEKEKEDFEK	VILEDVAMLH	IKPDQFTYTS	DHFETIMKYA	EKLIQEGKAY	VDDTPAEQMK
301	311	321	331	341	351	361	371	381	391
AEREQRIDSK	HRKNPIEKNL	QMWEEMKKGS	QFGQSCCLRA	KIDMSSNNGC	MRDPTLYRCK	IQPHPRTGNK	YNVYPTYDFA	CPIVDSIEGV	THALRTTEYH
401	411	421	431	441	451	461	471	481	491
DRDEQFYWII	EALGIRKPYI	WEYSRLNLNN	TVLSKRKLTW	FVNEGLVDGW	DDPRFPTVRG	VLRRGMTVEG	LKQFIAAQGS	SRSVVNMEWD	KIWAFNKKVI
501	511	521	531	541	551	561	571	581	591
DPVAPRYVAL	LKKEVIPVNV	PEAQEEMKEV	AKHPKNPEVG	LKPVWYSPKV	FIEGADAETF	SEGEMVTFIN	WGNLNITKIH	KNADGKIISL	DAKLNLENKD
601	611	621	631	641	651	661	671	681	691
YKKTTKVTWL	AETTHALPIP	VICVTYEHLI	TKPVLGKDED	FKQYVNKNSK	HEELMLGDPC	LKDLKKGDII	QLQRRGFFIC	DQPYEPVSPY	SCKEAPCVLI
701	711	721	731	741	751	761	771	781	791
YIPDGHTKEM	PTSGSKEKTK	VEATKNETSA	PFKERPTPSL	NNNCTTSEDS	LVLYNRVAVQ	GDVVRELKAK	KAPKEDVDAA	VKQLLSLKAE	YKEKTGQEYK
801	811	821	831	841	851	861	871	881	891
PGNPPAEIGQ	NISSNSSASI	LESKSLYDEV	AAQGEVVRKL	KAEKSPKAKI	NEAVECLLSL	KAQYKEKTGK	EYIPGQPPLS	QSSDSSPTRN	SEPAGLETPE
901	911	921	931	941	951	961	971	981	991
AKVLFDKVAS	QGEVVRKLKT	EKAPKDQVDI	AVQELLQLKA	QYKSLIGVEY	KPVSATGAED	KDKKKKEKEN	KSEKQNKPQK	QNDGQRKDPS	KNQGGGLSSS
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
GAGEGQGPKK	QTRLGLEAKK	EENLADWYSQ	VITKSEMIEY	HDISGCYILR	PWAYAIWEAI	KDFFDAEIKK	LGVENCYFPM	FVSQSALEKE	KTHVADFAPE
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
VAWVTRSGKT	ELAEPIAIRP	TSETVMYPAY	AKWVQSHRDL	PIKLNQWCNV	VRWEFKHPQP	FLRTREFLWQ	EGHSAFATME	EAAEEVLQIL	DLYAQVYEEL
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
LAIPVVKGRK	TEKEKFAGGD	YTTTIEAFIS	ASGRAIQGGT	SHHLGQNFSK	MFEIVFEDPK	IPGEKQFAYQ	NSWGLTTRTI	GVMTMVHGDN	MGLVLPPRVA
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
CVQVVIIPCG	ITNALSEEDK	EALIAKCNDY	RRRLLSVNIR	VRADLRDNYS	PGWKFNHWEL	KGVPIRLEVG	PRDMKSCQFV	AVRRDTGEKL	TVAENEAETK
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
LQAILEDIQV	TLFTRASEDL	KTHMVVANTM	EDFQKILDSG	KIVQIPFCGE	IDCEDWIKKT	TARDQDLEPG	APSMGAKSLC	IPFKPLCELQ	PGAKCVCGKN
1501	1511
PAKYYTLFGR	SY

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.

UniProtKB-P07814

Glycosites

Sequence

Reference