Contains      

UniProtKB-P08648

Protein Integrin alpha-5
Gene ITGA5
Status Reviewed
Source 22Rv1 cell line (prostate cancer); Bladder tumor; Breast Cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); LNCap/PC3 cell lines (Prostate cancer)PBMC Macrophage cells; Liver; Liver tumor (HCC); Lymphocytes; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); OVCAR-3 cell line (Ovarian cancer)PBMC Macrophage cells; Ovarian tumor; Ovarian tumorJurkat T cell line; Pancreatic islets; Plasma; Platelet; Platelet Plasma membranes; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); SKOV-3 cell line (Ovarian cancer)Jurkat T cell line; Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); prostate cancer cell lines; umbilical vein Endothelial CellsJurkat T cell line
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
84 VGAPKAnTSQPGVL 2014(33);
84 AnTSQPGVLQGGAVY 2014(33);
84 AnTSQPGVLQGGAVYLCPW 2014(33);
84 VGAPKAnTSQPGVLQGGAVY 2014(33);
84 AnTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSK 2012(52); 2014(15); 2015(1); 2015(12); 2015(unpublished);2007(unpublished);2007;
84 AnTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSR 2011(58);
182 STDnFTR 2014(33);
182 LSTDnFTR 2014(33);
182 YLSTDnFTR 2015(unpublished);
182 LSTDnFTRIL 2014(33);
182 TCYLSTDnFTR 2014(33);
182 LSTDnFTRILEY 2014(33);
182 PVGTCYLSTDnFTR 2007(75); 2014(14); 2014(33);
182 LSTDnFTRILEYAPCR 2014(33);
182 EPLSDPVGTCYLSTDnFTR 2011(57); 2012(48); 2012(52); 2013(34); 2014(22); 2014(33); 2015(unpublished);2015(9);
182 LSTDnFTRILEYAPCRSDF 2014(33);
182 KEPLSDPVGTCYLSTDnFTR 2014(33);
182 TEKEPISDPVGTCYISTDnFTR 2014(32); 2014(32);
182 TEKEPLSDPVGTCYLSTDnFTR 2007(74); 2009(62); 2009(62); 2009(62); 2009(67); 2011(58); 2012(52); 2013(34); 2013(34); 2014(13); 2014(14); 2014(15); 2014(17); 2014(18); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(Unpublished ); 2015;2007;2007;2007 ;
182 AHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDnFTR 2011(58);
297 VAGVPKGnLTY 2014(33);
297 GnITYGYVTIINGSDIR 2014(32); 2014(32);
297 GnLTYGYVTILNGSDIR 2007(72); 2007(74); 2007(75); 2009(62); 2009(62); 2009(62); 2009(64); 2011(57); 2011(58); 2012(48); 2012(50); 2012(52); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2015;2015;2007;2007;
297 SGDDTEDFVAGVPKGnLTY 2014(33);
297 SVAVGEFSGDDTEDFVAGVPKGnLTY 2014(33);
307 ILnGSDIR 2014(33);
307 VTILnGSDIR 2014(33);
307 ILnGSDIRSLY 2014(33);
307 GYVTILnGSDIR 2014(33);
307 VTILnGSDIRSLY 2014(33);
307 GYVTILnGSDIRSLY 2014(33);
307 GNITYGYVTIInGSDIR 2014(32); 2014(32);
307 GNLTYGYVTILnGSDIR 2007(72); 2007(74); 2007(75); 2009(62); 2009(62); 2009(62); 2009(64); 2011(57); 2011(58); 2012(48); 2012(50); 2012(52); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2015;2015;2007;2007;
316 SLYnFSGE 2014(33);
316 nFSGEQMASY 2014(33);
316 nFSGEQMASYF 2014(33);
316 SLYnFSGEQMASY 2014(33);
316 SLYnFSGEQMASYF 2014(33);
593 nESEFR 2014(22); 2015(unpublished);
593 IYLRnESEFR 2007(75); 2012(48); 2013(34); 2014(13); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2007;2007;
593 IYLRnESEFRDK 2011(57);
609 LSPIHIALnF 2014(33);
609 DKLSPIHIALnF 2014(33);
609 nFSLDPQAPVDSHGLRPALHYQSK 2015(1);
609 LSPIHIALnFSLDPQAPVDSHGLRPALHYQSK 2015(unpublished);2015(unpublished);2015(2);
675 NALnLTFH 2014(33);
675 LGDKNALnL 2014(33);
675 NALnLTFHAQ 2014(33);
675 LGDKNALnLTF 2014(33);
675 NALnLTFHAQNVGE 2014(33);
675 NALnLTFHAQNVGEGGAYE 2014(33);
675 LGDKNALnLTFHAQNVGEGGAY 2014(33);
675 NAInITFHAQNVGEGGAYEAEIR 2014(32);
675 NALnLTFHAQNVGEGGAYEAELR 2007(75); 2009(64); 2011(57); 2012(48); 2012(52); 2013(34); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2007;2007;
712 SGLVRHPGnF 2014(33);
712 VRHPGnFSSL 2014(33);
712 HPGnFSSLSCD 2014(33);
712 HPGnFSSLSCDY 2014(33);
712 HPGnFSSLSCDYF 2014(33);
712 SGLVRHPGnFSSL 2014(33);
712 GnFSSLSCDYFAVNQSR 2015(unpublished);
712 HPGnFSSLSCDYFAVNQSR 2007(74); 2011(58); 2012(48); 2012(52); 2013(34); 2013(34); 2014(14); 2014(22); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(12);
712 VTAPPEAEYSGLVRHPGnFSSLSCDYFAVNQSR 2015(12);
724 AVnQSRL 2014(33);
724 FAVnQSR 2014(33);
724 AVnQSRLL 2014(33);
724 YFAVnQSR 2014(33);
724 FAVnQSRLL 2014(33);
724 SCDYFAVnQSR 2014(33);
724 SCDYFAVnQSRL 2014(33);
724 SCDYFAVnQSRLL 2014(33);
724 AVnQSRLLVCDLGNPM 2014(33);
724 GNFSSLSCDYFAVnQSR 2015(unpublished);
724 HPGNFSSLSCDYFAVnQSR 2007(74); 2011(58); 2012(48); 2012(52); 2013(34); 2013(34); 2014(14); 2014(22); 2014(25); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(12);
724 VTAPPEAEYSGLVRHPGNFSSLSCDYFAVnQSR 2015(12);
773 nNSQSDVVSFR 2015(1);
773 IISKNInNSQSDV 2013(37);
773 NLnNSQSDVVSFR 2007(74); 2007(75); 2009(62); 2009(62); 2009(62); 2009(64); 2009(67); 2011(55); 2011(57); 2011(58); 2012(48); 2012(50); 2012(52); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;2007;2007;
773 SKNLnNSQSDVVSF 2014(33);
773 DFQILSKNLnNSQSDVVSF 2014(33);
868 nCTTNHPINPK 2012(48); 2014(33); 2015(1);
868 GLnCTTNHPINPK 2012(48); 2014(14); 2015(unpublished);
868 TRVTGInCTTNHP 2013(37);
868 TGLnCTTNHPINPK 2012(48); 2014(14); 2015(unpublished);
868 VTGLnCTTNHPINPK 2007(75); 2009(62); 2009(67); 2011(57); 2011(58); 2012(48); 2012(52); 2013(34); 2013(34); 2013(40); 2013(44); 2014(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2015(1); 2015(8); 2015(10); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
868 VTRVTGLnCTTNHPINPK 2014(33);
868 VTRVTGLnCTTNHPINPKGL 2014(33);

Sequence

1112131415161718191
MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCP
101111121131141151161171181191
WGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAA
201211221231241251261271281291
GQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTY
301311321331341351361371381391
GYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLG
401411421431441451461471481491
DLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLT
501511521531541551561571581591
IFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDK
601611621631641651661671681691
LSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPE
701711721731741751761771781791
AEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKP
801811821831841851861871881891
EAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRS
901911921931941951961971981991
SASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLW
10011011102110311041
IIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.