Contains      

UniProtKB-P13473

Protein Lysosome-associated membrane glycoprotein 2
Gene LAMP2
Status Reviewed
Source ARO Cell Line (Thyroid Cance); Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Plasma; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate tumor; SKOV-3 cell line (Ovarian cancer); SW1990 cell line (Pancreatic cancer); Saliva; Serum; Spermatozoa; SpermatozoaJurkat T cell line; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); ovarian tumor; prostate cancer cell lines
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
32 LnLTDSE 2014(33);
32 LELnLTDSE 2014(33);
32 LnLTDSENATCL 2014(33);
32 ELnLTDSENATCL 2014(33);
32 LnLTDSENATCLY 2014(33);
32 ELnLTDSENATCLY 2014(33);
32 LELnLTDSENATCL 2014(33);
32 LELnLTDSENATCLY 2014(33);
32 LnLTDSENATCLYAK 2014(33);
32 ELnLTDSENATCLYAK 2014(33);
32 LELnLTDSENATCLYAK 2005( 81); 2007(75); 2007(54); 2011(57); 2011(48); 2012(52); 2012(14); 2014(33); 2014(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007;2007;
32 SYALELnLTDSENATCLYAK 2015(1);
38 nATCLYAK 2014(33); 2015(1);
38 SEnATCLYAK 2014(33);
38 LNLTDSEnATCL 2014(33);
38 ELNLTDSEnATCL 2014(33);
38 LNLTDSEnATCLY 2014(33);
38 ELNLTDSEnATCLY 2014(33);
38 LELNLTDSEnATCL 2014(33);
38 LELNLTDSEnATCLY 2014(33);
38 LNLTDSEnATCLYAK 2014(33);
38 ELNLTDSEnATCLYAK 2014(33);
38 LELNLTDSEnATCLYAK 2005( 81); 2007(75); 2007(54); 2011(57); 2011(48); 2012(52); 2012(14); 2014(33); 2014(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007;2007;
38 SYALELNLTDSEnATCLYAK 2015(1);
49 WQMnF 2014(33);
49 AKWQMnF 2014(33);
49 QMnFTVR 2014(33);
49 QMnFTVRY 2014(33);
49 WQMnFTVR 2003( 83); 2005(81); 2007(72); 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(55); 2011(57); 2011(58); 2011(3); 2012(47); 2012(48); 2012(52); 2012(34); 2013(40); 2013(43); 2013(13); 2014(14); 2014(15); 2014(16); 2014(17); 2014(22); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;2007;
49 AKWQMnFTVR 2014(33);
49 WQMnFTVRYE 2014(33);
49 YAKWQMnFTVRYE 2013(37);
49 QMnFTVRYETTNKTY 2014(33);
58 YETTnK 2015(unpublished);2015(2);
58 ETTnKTY 2014(33);
58 TVRYETTnK 2014(33);
58 YETTnKTYK 2007(72); 2013(34); 2013(34); 2013(45); 2014(15); 2014(16); 2014(33); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(12);
58 RYETTnKTYKT 2011(56);
58 TVRYETTnKTY 2014(33);
58 QMNFTVRYETTnKTY 2014(33);
58 ETTnKTYKTVTISDHGTVTY 2014(33);
75 nGSICGDDQNGPK 2015(1);
75 YnGSICGDDQNGPK 2014(33);
75 HGTVTYnGSICGDDQNGPK 2014(33);
75 TVTISDHGTVTYnGSICGDDQNGPK 2007(72); 2007(62); 2009(62); 2009(52); 2012(34); 2013(34); 2013(34); 2013(45); 2013(14); 2014(15); 2014(16); 2014(25); 2014(25); 2014(32); 2014(32); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2015;2007;2007;
101 SWIAnFTK 2011(57); 2014(33);
101 GFSWIAnFTK 2014(33);
101 IAnFTKAASTY 2014(33);
101 GPGFSWIAnFTK 2014(33);
101 GFSWIAnFTKAAS 2013(37);
101 SWIAnFTKAASTY 2014(33);
101 GPGFSWIAnFTKAASTY 2014(33);
101 IAVQFGPGFSWIAnFTK 2003( 83); 2005(81); 2007(72); 2007(75); 2009(62); 2009(62); 2009(63); 2009(64); 2009(67); 2011(58); 2012(52); 2012(53); 2013(34); 2013(43); 2013(45); 2014(15); 2014(16); 2014(17); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;2007;
123 nTTFPDAEDK 2015(1);
123 GDnTTFPDAEDK 2014(14); 2015(unpublished);
123 TGDnTTFPDAEDK 2014(33); 2015(unpublished);
123 NTGDnTTFPDAEDK 2012(48); 2014(33); 2015(unpublished);
123 YNTGDnTTFPDAEDK 2014(33);
123 SYNTGDnTTFPDAEDK 2011(57); 2014(14); 2014(33); 2015(unpublished);
123 NTGDnTTFPDAEDKGIL 2014(33);
123 FSYNTGDnTTFPDAEDKG 2009(64);
123 SFSYNTGDnTTFPDAEDK 2014(14); 2015(unpublished);
123 SYNTGDnTTFPDAEDKGIL 2014(33);
123 NTGDnTTFPDAEDKGILTVDEL 2014(33);
123 NTGDnTTFPDAEDKGILTVDELL 2014(33);
123 SIDSVSFSYNTGDnTTFPDAEDK 2011(57); 2014(33); 2015(unpublished);
123 NTGDnTTFPDAEDKGILTVDELLAIR 2007(75);
123 AASTYSIDSVSFSYNTGDnTTFPDAEDK 2005( 81); 2009(62); 2011(57); 2012(48); 2012(52); 2014(14); 2014(32); 2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007;2007;2007 ;
123 SYNTGDnTTFPDAEDKGILTVDELLAIR 2007(75); 2014(33);
123 AASTYSIDSVSFSYNTGDnTTFPDAEDKGILTVDELLAIR 2015(2);
179 VQnGTVSTN 2014(33);
179 VQnGTVSTNE 2014(33);
179 VQnGTVSTNEF 2014(33);
179 AFVQnGTVSTNE 2014(33);
179 AFVQnGTVSTNEF 2014(33);
179 nGTVSTNEFLCDK 2015(1);
179 VQAFVQnGTVSTNE 2014(33);
179 VQnGTVSTNEFLCDK 2011(57); 2012(48); 2014(33);
179 VLVQAFVQnGTVSTNE 2014(33);
179 AFVQnGTVSTNEFLCDK 2011(57);
179 VQnGTVSTNEFLCDKDK 2014(33);
179 VQAFVQnGTVSTNEFLCDK 2014(33);
179 VQAFVQnGTVSTNEFLCDKDK 2014(33);
179 NDVVQHYWDVLVQAFVQnGTVSTNE 2014(33);
179 KNDVVQHYWDVLVQAFVQnGTVSTNE 2014(33);
179 NDVVQHYWDVLVQAFVQnGTVSTNEFLCDK 2011(57); 2015(2);
229 SVNNGnDTCL 2014(33);
229 SVNNGnDTCLL 2014(33);
229 nDTCLLATMGLQL 2015(1);
229 EKPEAGTYSVNNGnDT 2007(75); 2011(57); 2012(48); 2014(14); 2015(unpublished);
229 EKPEAGTYSVNNGnDTCLLA 2011(57);
229 EKPEAGTYSVNNGnDTCLLATM 2011(57);
229 SVNNGnDTCLLATMGLQLNITQDK 2014(33);
229 AGTYSVNNGnDTCLLATMGLQLNITQDK 2014(33);
229 EKPEAGTYSVNNGnDTCIIATMGIQINITQDK 2014(32);
229 EKPEAGTYSVNNGnDTCLLATMGLQLNITQDK 2011(57); 2012(52); 2014(14); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);
242 LnITQDK 2014(33);
242 QLnITQDK 2014(33); 2015(unpublished);
242 LQLnITQDK 2007(unpublished);2007(unpublished);2007;
242 GLQLnITQDK 2011(57); 2012(3); 2014(14); 2014(33); 2015(unpublished);

Sequence

1112131415161718191
MVCFRLFPVPGSGLVLVCLVLGAVRSYALELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIA
101111121131141151161171181191
NFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPT
201211221231241251261271281291
IHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVN
301311321331341351361371381391
ISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIGL
401
KHHHAGYEQF

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
47Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen S-H, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K: Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Molecular & Cellular Proteomics 2012, 11:M112. 017558.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.