Contains      

UniProtKB-P14384

Protein Carboxypeptidase M
Gene CPM
Status Reviewed
Source Breast Cancer cell lines; Breast cancer cell lines; Breast cell lines; Breast tumor; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); LNCap/PC3 cell lines (Prostate cancer)PBMC Macrophage cells; Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); OVCAR-3 cell line (Ovarian cancer)PBMC Macrophage cells; Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Spermatozoa; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance)
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
38 LKTVAQnY 2014(33);
38 TVAQnYSSVTH 2014(33);
38 IKTVAQnYSSVTH 2013(37);
38 nYSSVTHLHSIGK 2015(1);
38 TVAQnYSSVTHLH 2014(33); 2015(unpublished);
38 LKTVAQnYSSVTHL 2014(33);
38 TVAQnYSSVTHIHSIGK 2013(43); 2014(16); 2014(32);
38 TVAQnYSSVTHLHSIGK 2007(62); 2009(52); 2012(34); 2013(34); 2013(34); 2013(38); 2013(45); 2013(14); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;2007;
115 ITNLInSTR 2014(33);
115 PEITNLInSTR 2014(33);
115 DPEITNIInSTR 2014(32);
115 DPEITNLInSTR 2007(52); 2012(22); 2014(33); 2014(1); 2015(unpublished);2015(unpublished);2015;2007;2007;
115 LVTSDGKDPEITNLInSTR 2014(33);
115 LVTSDGKDPEITNLInSTRIH 2014(33);
115 LIDYLVTSDGKDPEITNLInSTR 2012(48); 2015(unpublished);
115 ELLLHLIDYLVTSDGKDPEITNLInSTR 2009(62); 2009(62); 2009(64); 2013(45); 2014(33); 2015(1); 2015(2); 2015(12);
164 FPDAFEYNnVSR 2014(33);
164 DAFEYNnVSRQPE 2013(37);
164 NFPDAFEYNnVSR 2007(75); 2007(62); 2009(62); 2009(64); 2009(3); 2012(48); 2012(52); 2012(34); 2013(45); 2013(14); 2014(15); 2014(16); 2014(17); 2014(22); 2014(32); 2014(32); 2014(33); 2014(1); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;2007;
164 nVSRQPETVAVMK 2015(1);
164 NFPDAFEYNnVSRQPETVAVMK 2013(38); 2015(1); 2015(12);
164 DLNRNFPDAFEYNnVSRQPETVAVM 2014(33);
363 IInVTVPGHD 2014(33);
363 IInVTVPGHDPH 2014(33);
363 nVTVPGHDPHITK 2015(1);
363 IInVTVPGHDPHITK 2007(48); 2012(33); 2014(unpublished);2015(unpublished);2007;2007;
363 LLLLPGSYIInVTVPGHDPHITK 2015(unpublished);
363 IInVTVPGHDPHITKVIIPEKSQNF 2014(33);
363 YYLLLLPGSYIInVTVPGHDPHITK 2014(33);
363 YGEYYLLLLPGSYIInVTVPGHDPHITK 2015(unpublished);
363 TNKYGEYYLLLLPGSYIInVTVPGHDPHITK 2015(unpublished);
384 SQnFSALK 2012(52); 2013(45); 2014(14); 2014(17); 2014(22); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
384 SQnFSALKK 2012(52); 2013(34); 2014(14); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
384 IPEKSQnFSAIKK 2013(37);
384 VIIPEKSQnFSALK 2015(12);
384 VIIPEKSQnFSAIKK 2014(16);
384 VIIPEKSQnFSALKK 2014(33); 2015(1); 2015(12);
384 IINVTVPGHDPHITKVIIPEKSQnF 2014(33);

Sequence

1112131415161718191
MDFPCLWLGLLLPLVAALDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYL
101111121131141151161171181191
VTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPF
201211221231241251261271281291
DNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNN
301311321331341351361371381391
KASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQL
401411421431441
DSIPVSNPSCPMIPLYRNLPDHSAATKPSLFLFLVSLLHIFFK

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.