| Protein | Aminopeptidase N |
| Gene | ANPEP |
| Status | Reviewed |
| Source | Bladder tumor; Breast Cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCCLM3 cell line (Liver); Hela cell line (cervical cancer); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; PBMC Macrophage cells; Pancreatic islets; Plasma; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Saliva; Serum; Spermatozoa; Urine; ovarian tumor; plasma; prostate cancer cell lines; umbilical vein Endothelial Cells |
| Years | 2005-20072007 |
| Site | Identified Peptides | Year(Publication ID) |
|---|---|---|
| 42 | NAnSSPVASTTPSASATTNPASATTLDQSK | 2014(33); |
| 128 | SKKLnY | 2014(33); |
| 128 | KLnYTLSQ | 2014(33); |
| 128 | nYTLSQGHR | 2014(33); |
| 128 | KLnYTLSQGH | 2012(48); |
| 128 | LnYTLSQGHR | 2005( 81); 2007(55); 2011(48); 2012(52); 2012(34); 2013(45); 2013(14); 2014(22); 2014(33); 2014(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007; |
| 128 | KInYTISQGHR | 2014(16); 2014(32); |
| 128 | KLnYTLSQGHR | 2007(72); 2009(64); 2012(48); 2012(52); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(18); 2014(19); 2014(22); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12); |
| 128 | nYTLSQGHRVVL | 2014(33); |
| 128 | IHSKKInYTISQG | 2013(37); |
| 128 | KLnYTLSQGHRVVLR | 2014(33); |
| 128 | TCKEATDVIIIHSKKLnY | 2014(33); |
| 128 | TCKEATDVIIIHSKKLnYTLSQGHRVVL | 2014(33); |
| 234 | nITLIHPK | 2014(33); 2015(1); |
| 234 | FnITLIHPK | 2012(48); 2014(14); 2014(33); |
| 234 | EFnITLIHPK | 2012(48); |
| 234 | nITLIHPKDL | 2014(33); |
| 234 | AEFnITIIHPK | 2014(16); 2014(32); 2014(32); |
| 234 | AEFnITLIHPK | 2005( 81); 2007(72); 2007(63); 2009(64); 2009(56); 2011(48); 2012(52); 2012(34); 2013(34); 2013(40); 2013(42); 2013(44); 2013(45); 2013(45); 2013(14); 2014(17); 2014(19); 2014(22); 2014(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;2007;2007;2007; |
| 234 | AMKAEFnITIIHP | 2013(37); |
| 234 | nITLIHPKDLTAL | 2014(33); |
| 234 | SFPCFDEPAMKAEFnITIIHPK | 2013(43); |
| 234 | SFPCFDEPAMKAEFnITLIHPK | 2012(52); |
| 265 | nVTEFHTTPK | 2015(1); |
| 265 | WnVTEFHTTPK | 2014(33); |
| 265 | PEDPNWnVTEFHT | 2013(37); |
| 265 | PNWnVTEFHTTPK | 2012(48); 2014(33); |
| 265 | DPNWnVTEFHTTPK | 2014(33); |
| 265 | STPLPEDPNWnVTE | 2014(33); |
| 265 | GPSTPLPEDPNWnVT | 2014(33); |
| 265 | GPSTPLPEDPNWnVTE | 2014(33); |
| 265 | GPSTPLPEDPNWnVTEF | 2012(48); 2014(33); |
| 265 | GPSTPLPEDPNWnVTEFH | 2014(33); |
| 265 | PKGPSTPLPEDPNWnVTE | 2009(64); |
| 265 | TPLPEDPNWnVTEFHTTPK | 2014(33); |
| 265 | MLPKGPSTPLPEDPNWnVTE | 2009(64); |
| 265 | STPLPEDPNWnVTEFHTTPK | 2014(33); 2015(unpublished); |
| 265 | MLPKGPSTPLPEDPNWnVTEF | 2014(33); |
| 265 | PSTPLPEDPNWnVTEFHTTPK | 2014(33); |
| 265 | GPSTPIPEDPNWnVTEFHTTPK | 2013(43); 2014(16); 2014(32); |
| 265 | GPSTPLPEDPNWnVTEFHTTPK | 2005( 81); 2007(72); 2007(64); 2009(54); 2011(48); 2012(52); 2012(34); 2013(34); 2013(34); 2013(40); 2013(42); 2013(45); 2013(13); 2014(14); 2014(35); 2014(17); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(UnpublishedUnpublished ); 2015;2015;2007;2007;2007;2007;2007;20072007 ; |
| 265 | SNMLPKGPSTPLPEDPNWnVTEF | 2014(33); |
| 265 | PKGPSTPLPEDPNWnVTEFHTTPK | 2014(33); |
| 265 | SNMLPKGPSTPLPEDPNWnVTEFHTTPKMSTY | 2014(33); |
| 319 | ALnVTGPIL | 2014(33); |
| 319 | ALnVTGPILN | 2014(33); |
| 319 | ALnVTGPILNF | 2014(33); |
| 319 | ALnVTGPILNFF | 2014(33); |
| 319 | GHGDYALnVTGPIL | 2014(33); |
| 319 | PSAIAAGHGDYALnV | 2014(14); |
| 319 | ALnVTGPILNFFAGHY | 2014(33); |
| 319 | ALnVTGPILNFFAGHYDTPYPLPK | 2014(33); |
| 319 | IWARPSAIAAGHGDYALnVTGPIL | 2014(33); |
| 319 | AAGHGDYALnVTGPILNFFAGHYDTPYPLPK | 2007(unpublished);2007(unpublished); |
| 319 | PSAIAAGHGDYAInVTGPIINFFAGHYDTPYPIPK | 2013(43); |
| 319 | PSAIAAGHGDYALnVTGPILNFFAGHYDTPYPLPK | 2012(52); 2014(33); 2015(unpublished); |
| 319 | IWARPSAIAAGHGDYALnVTGPILNFFAGHYDTPYPLPK | 2014(33); |
| 527 | DHLQEAVNnR | 2014(33); |
| 527 | WDHLQEAVNnR | 2007(unpublished);2007(unpublished);2007; |
| 527 | LWDHLQEAVNnR | 2014(33); |
| 527 | NLWDHLQEAVNnR | 2014(33); |
| 527 | LNLWDHLQEAVNnR | 2014(33); |
| 527 | DHLQEAVNnRSIQLPT | 2014(33); |
| 527 | LNLWDHLQEAVNnRSIQLPT | 2014(33); |
| 527 | AYQNTIYLNLWDHLQEAVNnR | 2014(33); |
| 573 | SnVTRPSEF | 2014(33); |
| 573 | LLDPDSnVTR | 2014(33); |
| 573 | PDSnVTRPSE | 2014(33); |
| 573 | PDSnVTRPSEF | 2014(33); |
| 573 | DPDSnVTRPSEF | 2014(33); |
| 573 | HFLLDPDSnVTR | 2014(33); |
| 573 | LDPDSnVTRPSE | 2009(64); |
| 573 | LDPDSnVTRPSEF | 2014(33); |
| 573 | LLDPDSnVTRPSE | 2009(64); 2014(33); |
| 573 | HFLLDPDSnVTRPS | 2014(33); |
| 573 | LLDPDSnVTRPSEF | 2014(33); |
| 573 | FLLDPDSnVTRPSEF | 2014(33); |
| 573 | HFLLDPDSnVTRPSE | 2013(42); 2014(33); |
| 573 | HFLLDPDSnVTRPSEF | 2014(33); |
| 573 | LLDPDSnVTRPSEFNY | 2014(33); |
| 573 | HFLLDPDSnVTRPSEFNY | 2014(33); |
| 573 | LLDPDSnVTRPSEFNYVW | 2014(33); |
| 573 | PDSnVTRPSEFNYVWIVPITSIR | 2007(33); 2014(unpublished);2007(unpublished);2007; |
| 573 | LLDPDSnVTRPSEFNYVWIVPITSIR | 2014(33); |
| 573 | HFLLDPDSnVTRPSEFNYVWIVPITSIR | 2014(33); |
| 573 | WTLQMGFPVITVDTSTGTLSQEHFLLDPDSnVTR | 2014(33); 2015(unpublished); |
| 625 | NLnVTGY | 2014(33); |
| 625 | nVTGYYR | 2015(1); |
| 625 | LNLnVTGY | 2014(33); |
| 625 | LnVTGYYR | 2014(33); |
| 625 | NLnVTGYY | 2014(33); |
| 625 | LNLnVTGYY | 2014(33); |
| 625 | NLnVTGYYR | 2007(19); 2014(33); 2014(unpublished);2015(unpublished);2007;2007; |
| 625 | LNLnVTGYYR | 2014(33); |
| 625 | VLLNLnVTGY | 2014(33); |
| 625 | VLLNLnVTGYY | 2014(33); |
| 625 | WVLLNLnVTGY | 2014(33); |
| 625 | WVLLNLnVTGYYR | 2014(33); |
| 625 | AQNDIFSTSGNEWVIINInVTGYYR | 2013(43); |
| 625 | AQNDLFSTSGNEWVLLNLnVTGYYR | 2012(52); 2015(unpublished);2015(2); 2015(unpublished); |
| 1 | 11 | 21 | 31 | 41 | 51 | 61 | 71 | 81 | 91 |
| MAKGFYISKS | LGILGILLGV | AAVCTIIALS | VVYSQEKNKN | ANSSPVASTT | PSASATTNPA | SATTLDQSKA | WNRYRLPNTL | KPDSYRVTLR | PYLTPNDRGL |
| 101 | 111 | 121 | 131 | 141 | 151 | 161 | 171 | 181 | 191 |
| YVFKGSSTVR | FTCKEATDVI | IIHSKKLNYT | LSQGHRVVLR | GVGGSQPPDI | DKTELVEPTE | YLVVHLKGSL | VKDSQYEMDS | EFEGELADDL | AGFYRSEYME |
| 201 | 211 | 221 | 231 | 241 | 251 | 261 | 271 | 281 | 291 |
| GNVRKVVATT | QMQAADARKS | FPCFDEPAMK | AEFNITLIHP | KDLTALSNML | PKGPSTPLPE | DPNWNVTEFH | TTPKMSTYLL | AFIVSEFDYV | EKQASNGVLI |
| 301 | 311 | 321 | 331 | 341 | 351 | 361 | 371 | 381 | 391 |
| RIWARPSAIA | AGHGDYALNV | TGPILNFFAG | HYDTPYPLPK | SDQIGLPDFN | AGAMENWGLV | TYRENSLLFD | PLSSSSSNKE | RVVTVIAHEL | AHQWFGNLVT |
| 401 | 411 | 421 | 431 | 441 | 451 | 461 | 471 | 481 | 491 |
| IEWWNDLWLN | EGFASYVEYL | GADYAEPTWN | LKDLMVLNDV | YRVMAVDALA | SSHPLSTPAS | EINTPAQISE | LFDAISYSKG | ASVLRMLSSF | LSEDVFKQGL |
| 501 | 511 | 521 | 531 | 541 | 551 | 561 | 571 | 581 | 591 |
| ASYLHTFAYQ | NTIYLNLWDH | LQEAVNNRSI | QLPTTVRDIM | NRWTLQMGFP | VITVDTSTGT | LSQEHFLLDP | DSNVTRPSEF | NYVWIVPITS | IRDGRQQQDY |
| 601 | 611 | 621 | 631 | 641 | 651 | 661 | 671 | 681 | 691 |
| WLIDVRAQND | LFSTSGNEWV | LLNLNVTGYY | RVNYDEENWR | KIQTQLQRDH | SAIPVINRAQ | IINDAFNLAS | AHKVPVTLAL | NNTLFLIEER | QYMPWEAALS |
| 701 | 711 | 721 | 731 | 741 | 751 | 761 | 771 | 781 | 791 |
| SLSYFKLMFD | RSEVYGPMKN | YLKKQVTPLF | IHFRNNTNNW | REIPENLMDQ | YSEVNAISTA | CSNGVPECEE | MVSGLFKQWM | ENPNNNPIHP | NLRSTVYCNA |
| 801 | 811 | 821 | 831 | 841 | 851 | 861 | 871 | 881 | 891 |
| IAQGGEEEWD | FAWEQFRNAT | LVNEADKLRA | ALACSKELWI | LNRYLSYTLN | PDLIRKQDAT | STIISITNNV | IGQGLVWDFV | QSNWKKLFND | YGGGSFSFSN |
| 901 | 911 | 921 | 931 | 941 | 951 | 961 | |||
| LIQAVTRRFS | TEYELQQLEQ | FKKDNEETGF | GSGTRALEQA | LEKTKANIKW | VKENKEVVLQ | WFTENSK |
| ID | Publication |
|---|---|
| 1 | Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication. |
| 2 | Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763. |
| 12 | Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5. |
| 13 | Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153. |
| 14 | Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768. |
| 16 | Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251. |
| 17 | Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105. |
| 18 | Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716. |
| 19 | Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206. |
| 22 | Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941. |
| 32 | Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837. |
| 33 | Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721. |
| 34 | Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615. |
| 35 | Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674. |
| 37 | Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171. |
| 40 | Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8. |
| 42 | Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450. |
| 43 | Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759. |
| 44 | Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978. |
| 45 | Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991. |
| 48 | Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608. |
| 52 | Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667. |
| 54 | Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687. |
| 55 | Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019. |
| 56 | Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061. |
| 63 | Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672. |
| 64 | Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661. |
| 72 | Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+. |