Contains      

UniProtKB-P15586

Protein N-acetylglucosamine-6-sulfatase
Gene GNS
Status Reviewed
Source 22Rv1 cell line (prostate cancer); 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Bladder stromal cell line; Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HCCLM3 cell line (Liver); HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HepG2 cell line (Liver); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; PBMC Macrophage cells; Pancreatic islets; Platelet; Prostate; Prostate cancer cell lines; Prostate cancer metastasis to liver; Prostate stromal cell line; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Spermatozoa; TPC-1 Cell Line (Thyroid Cance); Urine; UrineJurkat T cell line; XTC-1 Cell Line (Thyroid Cance); ovarian tumor; umbilical vein Endothelial Cells
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
111 nNTLEGNCSSK 2015(1);
111 HNHHVVnNTIEGN 2013(37);
111 nNTIEGNCSSKSW 2013(37);
111 YPHNHHVVnNTLE 2014(33);
111 HVVnNTLEGNCSSK 2012(48);
111 YPHNHHVVnNTIEGNCSSK 2014(16);
111 YPHNHHVVnNTLEGNCSSK 2007(62); 2009(62); 2009(62); 2009(62); 2009(57); 2011(48); 2012(52); 2012(34); 2013(38); 2013(38); 2013(40); 2013(45); 2013(13); 2014(14); 2014(18); 2014(33); 2014(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;2007;
111 ASILTGKYPHNHHVVnNTLE 2014(33);
111 ASIITGKYPHNHHVVnNTIEGNCSSK 2013(43);
111 ASILTGKYPHNHHVVnNTLEGNCSSK 2012(52); 2014(18); 2015(12);
117 NNTLEGnCSSK 2015(1);
117 NNTIEGnCSSKSW 2013(37);
117 HVVNNTLEGnCSSK 2012(48);
117 YPHNHHVVNNTIEGnCSSK 2014(16);
117 YPHNHHVVNNTLEGnCSSK 2007(62); 2009(62); 2009(62); 2009(62); 2009(57); 2011(48); 2012(52); 2012(34); 2013(38); 2013(38); 2013(40); 2013(45); 2013(13); 2014(14); 2014(18); 2014(33); 2014(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;2007;
117 ASIITGKYPHNHHVVNNTIEGnCSSK 2013(43);
117 ASILTGKYPHNHHVVNNTLEGnCSSK 2012(52); 2014(18); 2015(12);
183 YYnYTISINGK 2014(16); 2014(32);
183 YYnYTLSINGK 2003( 83); 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(64); 2009(65); 2009(65); 2009(67); 2009(54); 2011(57); 2011(48); 2012(50); 2012(52); 2012(38); 2013(40); 2013(13); 2014(14); 2014(19); 2014(22); 2014(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;2007;
183 KNSKYYnYTISIN 2013(37);
183 NSKYYnYTLSINGK 2012(52); 2015(2);
198 KHGEnYSVDYL 2007(unpublished);2007(unpublished);2007(unpublished);2007;
198 HGEnYSVDYLTDVLANVSLDFLDYK 2011(57); 2013(38); 2014(33); 2015(2);
198 KHGEnYSVDYITDVIANVSIDFIDYK 2013(43); 2014(16);
198 KHGEnYSVDYLTDVLANVSLDFLDYK 2009(62); 2014(33); 2015(unpublished);2015(2);
198 HGEnYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQK 2013(42);
210 nVSLDFLDYK 2014(14);
210 YLTDVLAnVS 2014(33);
210 TDVLAnVSLDFLDYK 2007(unpublished);2007(unpublished);2007(unpublished);
210 HGENYSVDYLTDVLAnVSLDFLDYK 2011(57); 2013(38); 2014(33); 2015(2);
210 KHGENYSVDYITDVIAnVSIDFIDYK 2013(43); 2014(16);
210 KHGENYSVDYLTDVLAnVSLDFLDYK 2009(62); 2014(33); 2015(unpublished);2015(2);
210 HGENYSVDYLTDVLAnVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQK 2013(42);
279 TPMTnSSIQFL 2014(33);
279 nSSIQFLDNAFR 2015(1);
279 TPMTnSSIQFLD 2014(33);
279 AKTPMTnSSIQFI 2013(37);
279 QAKTPMTnSSIQF 2014(33);
279 TnSSIQFLDNAFR 2014(14); 2014(33); 2015(unpublished);
279 MTnSSIQFLDNAFR 2014(14); 2015(unpublished);2015(unpublished);
279 TPMTnSSIQFIDNAFR 2014(32); 2014(32);
279 TPMTnSSIQFLDNAFR 2003( 83); 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(65); 2009(65); 2009(67); 2009(58); 2011(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(40); 2013(45); 2013(13); 2014(14); 2014(17); 2014(18); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007;2007;2007;2007;2007;2007;2007 ;
279 TPMTnSSIQFIDNAFRK 2013(43); 2014(16);
279 TPMTnSSIQFLDNAFRK 2012(52); 2014(18); 2015(2); 2015(unpublished);2015(unpublished);2015(12);
279 QAKTPMTnSSIQFLDNAFR 2014(18); 2014(33);
317 FTGELnNTY 2009(64);
317 EFTGELnNTY 2014(33);
317 LEFTGELnNTY 2014(33);
317 LEFTGELnNTYI 2007(unpublished);2007(unpublished);
317 RLEFTGELnNTY 2011(57); 2014(33);
317 RLEFTGELnNTYIFYTSDNGYHTGQF 2015(unpublished);
317 LEFTGELnNTYIFYTSDNGYHTGQFSLPIDK 2009(62); 2015(unpublished);2015(2);
317 RLEFTGELnNTYIFYTSDNGYHTGQFSLPIDK 2015(unpublished);2015(2);
317 RLEFTGELnNTYIFYTSDNGYHTGQFSLPIDKR 2012(52); 2015(2);
362 GIKPnQTSK 2012(48); 2014(14); 2015(unpublished);2015(unpublished);
362 PGIKPnQTSK 2012(48); 2014(14);
362 GPGIKPnQTSK 2011(57); 2012(48); 2012(50); 2012(52); 2013(34); 2013(34); 2013(34); 2013(40); 2014(16); 2014(18); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
362 GPGIKPnQTSKMI 2013(37);
387 IAGYDLnK 2014(33);
387 IAGYDLnKTQ 2009(64);
387 DLnKTQMDGMSL 2014(33);
387 nKTQMDGMSLLPILR 2015(1);
387 DLnKTQMDGMSLLPIL 2014(33);
387 DLnKTQMDGMSLLPILR 2014(33);
387 NIDLGPTILDIAGYDLnK 2011(57);
387 DLnKTQMDGMSLLPILRGASNL 2014(33);
387 MIVANIDIGPTIIDIAGYDInK 2013(43);
387 MLVANIDLGPTILDIAGYDLnK 2007(62); 2009(62); 2009(62); 2009(57); 2011(52); 2012(33); 2014(2); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
387 MLVANIDLGPTILDIAGYDLnKTQMDGMSLLPILR 2012(52); 2015(unpublished);2015(2);
405 GASnITWR 2014(16); 2014(32); 2014(32);
405 GASnLTWR 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(57); 2011(52); 2012(34); 2013(40); 2013(44); 2013(45); 2013(13); 2014(17); 2014(18); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;2007;
405 IIRGASnITWRSD 2013(37);
405 DLNKTQMDGMSLLPILRGASnL 2014(33);
422 LVEYQGEGRnVTDPTCPS 2009(64);
422 nVTDPTCPSLSPGVSQCFPDCVCE 2014(33);
422 nVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVR 2014(33); 2015(unpublished);
449 nNTYACVR 2015(1);
449 DAYnNTYACVR 2014(33);
449 NVTDPTCPSLSPGVSQCFPDCVCEDAYnNTYACVR 2014(33); 2015(unpublished);
480 VYnLTADPDQITN 2014(33);
480 nITADPDQITNIAK 2012(48);
480 nLTADPDQITNIAK 2007(75); 2014(14); 2014(33); 2015(1); 2015(unpublished);
480 VYnLTADPDQITNIA 2014(33);
480 VYnLTADPDQITNIAK 2014(33);
480 VEVYnITADPDQITNIAK 2012(48);
480 nLTADPDQITNIAKTIDPELLGKMNY 2014(33);
480 TMSALWNLQYCEFDDQEVFVEVYnLTADPDQITNIAK 2015(2);

Sequence

1112131415161718191
MRLLPLAPGRLRRGSPRHLPSCSPALLLLVLGGCLGVFGVAAGTRRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILT
101111121131141151161171181191
GKYPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYS
201211221231241251261271281291
VDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVD
301311321331341351361371381391
DLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPIL
401411421431441451461471481491
RGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELL
501511521531541551
GKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.