Contains      

UniProtKB-P19022

Protein Cadherin-2
Gene CDH2
Status Reviewed
Source Breast Cancer cell lines; Breast cancer xenografts; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); HuH-7 cell line (Liver); Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; Lymphocytes; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Prostate; Prostate tumor; Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); umbilical vein Endothelial Cells
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
273 FLHQVWnGTVPE 2014(33);
273 nGTVPEGSKPGTY 2014(33);
273 VWnGTVPEGSKPGTY 2009(64); 2014(33);
273 HQVWnGTVPEGSKPGTY 2014(33);
325 MFTINnE 2014(33);
325 TINnETGDIITVAAGL 2014(33);
325 TINnETGDIITVAAGLDR 2014(33); 2015(unpublished);
325 IVSQAPSTPSPNMFTINnE 2014(33);
325 MFTINnETGDIITVAAGLDR 2011(57); 2014(33); 2015(unpublished);
325 TINnETGDIITVAAGLDREK 2011(57);
325 MFTINnETGDIITVAAGLDREK 2011(57);
325 TINnETGDIITVAAGLDREKVQQY 2014(33);
325 IVSQAPSTPSPNMFTINnETGDIITVAAGLDR 2007(62); 2009(57); 2011(45); 2013(33); 2014(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
325 IVSQAPSTPSPNMFTINnETGDIITVAAGIDREK 2014(16);
325 IVSQAPSTPSPNMFTINnETGDIITVAAGLDREK 2009(62); 2011(57); 2015(2); 2015(12);
325 YRIVSQAPSTPSPNMFTINnETGDIITVAAGLDREK 2013(38);
402 VDIIVAnLTVTDK 2011(57); 2014(33); 2015(unpublished);2015(unpublished);2015;2007;2007;
402 GEVPENRVDIIVAnL 2014(33);
402 YGEVPENRVDIIVAnL 2014(33);
402 YGEVPENRVDIIVAnLTVTDK 2011(57);
402 FYGEVPENRVDIIVAnLTVTDK 2011(57);
402 VDIIVAnITVTDKDQPHTPAWNAVYR 2014(16);
402 VDIIVAnLTVTDKDQPHTPAWNAVYR 2007(unpublished);2009(62); 2009(64); 2013(40); 2013(45); 2014(33); 2015(unpublished);2015(1); 2015(2);
402 NRVDIIVAnLTVTDKDQPHTPAWNAVYR 2014(33);
402 GEVPENRVDIIVAnLTVTDKDQPHTPAWNAVY 2014(33);
402 YGEVPENRVDIIVAnLTVTDKDQPHTPAWNAVY 2014(33);
572 NNIYnATFL 2014(33);
572 DRESPNVKNNIYnATF 2009(64); 2014(33);
572 DRESPNVKNNIYnATFL 2014(33);
651 nWTITR 2014(15); 2014(33); 2015(2);
651 RnWTITR 2013(40); 2014(13); 2014(15); 2014(16); 2014(33); 2015(unpublished);2015(2); 2015(12);
651 SPVTIKRnW 2014(33);
651 DLPLSPVTIKRnW 2014(33);
651 PVTIKRnWTITRI 2013(37);
651 AFDLPLSPVTIKRnW 2014(33);
692 SnISIIR 2014(16);
692 SnISILR 2012(48); 2013(40); 2013(44); 2013(45); 2014(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
692 EVPIIITDSGNPPKSnISIL 2014(33);
692 EVPIIITDSGNPPKSnISILR 2014(33);
692 AGIYEVPIIITDSGNPPKSnISILR 2014(33);
692 FIEAGIYEVPIIITDSGNPPKSnISIIR 2014(16);
692 FLEAGIYEVPIIITDSGNPPKSnISILR 2007(64); 2009(15); 2014(2); 2015(12); 2015(unpublished);2007(unpublished);2007;
692 IKFLEAGIYEVPIIITDSGNPPKSnISILR 2015(2);

Sequence

1112131415161718191
MCRIAGALRTLLPLLAALLQASVEASGEIALCKTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHA
101111121131141151161171181191
KFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGP
201211221231241251261271281291
GADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNAL
301311321331341351361371381391
NGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIV
401411421431441451461471481491
ANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPN
501511521531541551561571581591
PKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDIND
601611621631641651661671681691
NAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKV
701711721731741751761771781791
CQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDA
801811821831841851861871881891
IKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADM
901
YGGGDD

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.