Contains      

UniProtKB-P20702

Protein Integrin alpha-X
Gene ITGAX
Status Reviewed
Source Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); Human; Liver; Liver tumor (HCC); Ovarian tumor; Ovarian tumorPBMC Macrophage cells; PBMC Macrophage cells; Prostate; Prostate tumor; ovarian tumor
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
61 KITAAnQTGGLY 2014(33);
61 ITAAnQTGGLYQCGY 2007(unpublished);2007(unpublished);
385 LYPPnMSPTFINMSQENVDMRDSY 2014(33);
392 LYPPNMSPTFInMSQENVDMRDSY 2014(33);
697 SPRATFQETKnRSLSRVRVL 2015(6);
735 LnFTLVG 2014(33);
735 LnFTLVGK 2015(unpublished);
735 LnFTLVGKPL 2015(unpublished);
735 LnFTLVGKPLLAFR 2015(unpublished);2015(unpublished);
899 IIITAnVSSENNTPR 2014(16); 2014(32); 2014(32);
899 LLLTAnVSSENNTPR 2009(64); 2011(54); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015;2007;2007;
899 TAnVSSENNTPRTSKTTF 2014(33);
904 IIITANVSSEnNTPR 2014(16); 2014(32); 2014(32);
904 LLLTANVSSEnNTPR 2009(64); 2011(54); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015;2007;2007;
904 TANVSSEnNTPRTSKTTF 2014(33);
939 YInFSESEEK 2014(16); 2014(32);
939 YLnFSESEEK 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);
939 YLnFSESEEKE 2014(33);
939 YLnFSESEEKESHVAMHR 2014(33); 2015(unpublished);
1050 GnISFGWVR 2014(16); 2014(32); 2014(32);
1050 GnLSFGWVR 2014(33); 2015(unpublished);

Sequence

1112131415161718191
MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTT
101111121131141151161171181191
SPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTH
201211221231241251261271281291
FTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKE
301311321331341351361371381391
LNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDM
401411421431441451461471481491
RDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRG
501511521531541551561571581591
WRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLV
601611621631641651661671681691
DLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSL
701711721731741751761771781791
SRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLE
801811821831841851861871881891
LNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANV
901911921931941951961971981991
SSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSS
1001101110211031104110511061107110811091
EKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKY
1101111111211131114111511161
KVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK

Reference

ID Publication
6Kim DS, Hahn Y: The acquisition of novel N-glycosylation sites in conserved proteins during human evolution. Bmc Bioinformatics 2015, 16.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.