Detail

UniProtKB-P20908


Protein	Collagen alpha-1(V) chain
Gene	COL5A1
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; Breast tumor; Hela cell line (cervical cancer); Liver; Ovarian tumor; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
176	nVTLILDCK	2014(17); 2015(unpublished);2015(unpublished);2015(12);
176	KnVTLILDCK	2015(unpublished);2015(unpublished);2015(12);
176	ISVHKKnVTIIID	2013(37);
1672	SFKVYCnFTAGGS	2013(37);
1672	VYCnFTAGGSTCVFPDK	2014(14); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007;2007;
1672	VYCnFTAGGSTCVFPDKK	2012(3); 2013(34); 2013(34); 2014(33); 2015(unpublished);2015(12);
1741	LLSASAHQnVTYH	2015(unpublished);
1741	LLSASAHQnVTYHCY	2015(unpublished);
1741	LLSASAHQnVTYHCYQSVAWQDAATGSYDK	2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MDVHTRWKAR	SALRPGAPLL	PPLLLLLLWA	PPPSRAAQPA	DLLKVLDFHN	LPDGITKTTG	FCATRRSSKG	PDVAYRVTKD	AQLSAPTKQL	YPASAFPEDF
101	111	121	131	141	151	161	171	181	191
SILTTVKAKK	GSQAFLVSIY	NEQGIQQIGL	ELGRSPVFLY	EDHTGKPGPE	DYPLFRGINL	SDGKWHRIAL	SVHKKNVTLI	LDCKKKTTKF	LDRSDHPMID
201	211	221	231	241	251	261	271	281	291
INGIIVFGTR	ILDEEVFEGD	IQQLLFVSDH	RAAYDYCEHY	SPDCDTAVPD	TPQSQDPNPD	EYYTEGDGEG	ETYYYEYPYY	EDPEDLGKEP	TPSKKPVEAA
301	311	321	331	341	351	361	371	381	391
KETTEVPEEL	TPTPTEAAPM	PETSEGAGKE	EDVGIGDYDY	VPSEDYYTPS	PYDDLTYGEG	EENPDQPTDP	GAGAEIPTST	ADTSNSSNPA	PPPGEGADDL
401	411	421	431	441	451	461	471	481	491
EGEFTEETIR	NLDENYYDPY	YDPTSSPSEI	GPGMPANQDT	IYEGIGGPRG	EKGQKGEPAI	IEPGMLIEGP	PGPEGPAGLP	GPPGTMGPTG	QVGDPGERGP
501	511	521	531	541	551	561	571	581	591
PGRPGLPGAD	GLPGPPGTML	MLPFRFGGGG	DAGSKGPMVS	AQESQAQAIL	QQARLALRGP	AGPMGLTGRP	GPVGPPGSGG	LKGEPGDVGP	QGPRGVQGPP
601	611	621	631	641	651	661	671	681	691
GPAGKPGRRG	RAGSDGARGM	PGQTGPKGDR	GFDGLAGLPG	EKGHRGDPGP	SGPPGPPGDD	GERGDDGEVG	PRGLPGEPGP	RGLLGPKGPP	GPPGPPGVTG
701	711	721	731	741	751	761	771	781	791
MDGQPGPKGN	VGPQGEPGPP	GQQGNPGAQG	LPGPQGAIGP	PGEKGPLGKP	GLPGMPGADG	PPGHPGKEGP	PGEKGGQGPP	GPQGPIGYPG	PRGVKGADGI
801	811	821	831	841	851	861	871	881	891
RGLKGTKGEK	GEDGFPGFKG	DMGIKGDRGE	IGPPGPRGED	GPEGPKGRGG	PNGDPGPLGP	PGEKGKLGVP	GLPGYPGRQG	PKGSIGFPGF	PGANGEKGGR
901	911	921	931	941	951	961	971	981	991
GTPGKPGPRG	QRGPTGPRGE	RGPRGITGKP	GPKGNSGGDG	PAGPPGERGP	NGPQGPTGFP	GPKGPPGPPG	KDGLPGHPGQ	RGETGFQGKT	GPPGPPGVVG
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
PQGPTGETGP	MGERGHPGPP	GPPGEQGLPG	LAGKEGTKGD	PGPAGLPGKD	GPPGLRGFPG	DRGLPGPVGA	LGLKGNEGPP	GPPGPAGSPG	ERGPAGAAGP
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
IGIPGRPGPQ	GPPGPAGEKG	APGEKGPQGP	AGRDGLQGPV	GLPGPAGPVG	PPGEDGDKGE	IGEPGQKGSK	GDKGEQGPPG	PTGPQGPIGQ	PGPSGADGEP
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
GPRGQQGLFG	QKGDEGPRGF	PGPPGPVGLQ	GLPGPPGEKG	ETGDVGQMGP	PGPPGPRGPS	GAPGADGPQG	PPGGIGNPGA	VGEKGEPGEA	GEPGLPGEGG
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
PPGPKGERGE	KGESGPSGAA	GPPGPKGPPG	DDGPKGSPGP	VGFPGDPGPP	GEPGPAGQDG	PPGDKGDDGE	PGQTGSPGPT	GEPGPSGPPG	KRGPPGPAGP
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
EGRQGEKGAK	GEAGLEGPPG	KTGPIGPQGA	PGKPGPDGLR	GIPGPVGEQG	LPGSPGPDGP	PGPMGPPGLP	GLKGDSGPKG	EKGHPGLIGL	IGPPGEQGEK
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
GDRGLPGPQG	SSGPKGEQGI	TGPSGPIGPP	GPPGLPGPPG	PKGAKGSSGP	TGPKGEAGHP	GPPGPPGPPG	EVIQPLPIQA	SRTRRNIDAS	QLLDDGNGEN
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
YVDYADGMEE	IFGSLNSLKL	EIEQMKRPLG	TQQNPARTCK	DLQLCHPDFP	DGEYWVDPNQ	GCSRDSFKVY	CNFTAGGSTC	VFPDKKSEGA	RITSWPKENP
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
GSWFSEFKRG	KLLSYVDAEG	NPVGVVQMTF	LRLLSASAHQ	NVTYHCYQSV	AWQDAATGSY	DKALRFLGSN	DEEMSYDNNP	YIRALVDGCA	TKKGYQKTVL
1801	1811	1821	1831
EIDTPKVEQV	PIVDIMFNDF	GEASQKFGFE	VGPACFMG

Reference

ID	Publication
3	Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
12	Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14	Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
17	Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34	Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.