Detail

Contains

UniProtKB-P22314


Protein	Ubiquitin-like modifier-activating enzyme 1
Gene	UBA1
Status	Reviewed
Source	Breast cancer xenografts; HCC cell lines (Liver); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Platelet
Years	2013-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
44	nGSEADIDEGLYSR	2013(40); 2015(1); 2015(unpublished);
818	nASVDDSR	2015(1);
818	IHVSDQELQSAnASVDDSR	2015(unpublished);
818	IHVSDQELQSAnASVDDSRLEELK	2015(unpublished);2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MSSSPLSKKR	RVSGPDPKPG	SNCSPAQSVL	SEVPSVPTNG	MAKNGSEADI	DEGLYSRQLY	VLGHEAMKRL	QTSSVLVSGL	RGLGVEIAKN	IILGGVKAVT
101	111	121	131	141	151	161	171	181	191
LHDQGTAQWA	DLSSQFYLRE	EDIGKNRAEV	SQPRLAELNS	YVPVTAYTGP	LVEDFLSGFQ	VVVLTNTPLE	DQLRVGEFCH	NRGIKLVVAD	TRGLFGQLFC
201	211	221	231	241	251	261	271	281	291
DFGEEMILTD	SNGEQPLSAM	VSMVTKDNPG	VVTCLDEARH	GFESGDFVSF	SEVQGMVELN	GNQPMEIKVL	GPYTFSICDT	SNFSDYIRGG	IVSQVKVPKK
301	311	321	331	341	351	361	371	381	391
ISFKSLVASL	AEPDFVVTDF	AKFSRPAQLH	IGFQALHQFC	AQHGRPPRPR	NEEDAAELVA	LAQAVNARAL	PAVQQNNLDE	DLIRKLAYVA	AGDLAPINAF
401	411	421	431	441	451	461	471	481	491
IGGLAAQEVM	KACSGKFMPI	MQWLYFDALE	CLPEDKEVLT	EDKCLQRQNR	YDGQVAVFGS	DLQEKLGKQK	YFLVGAGAIG	CELLKNFAMI	GLGCGEGGEI
501	511	521	531	541	551	561	571	581	591
IVTDMDTIEK	SNLNRQFLFR	PWDVTKLKSD	TAAAAVRQMN	PHIRVTSHQN	RVGPDTERIY	DDDFFQNLDG	VANALDNVDA	RMYMDRRCVY	YRKPLLESGT
601	611	621	631	641	651	661	671	681	691
LGTKGNVQVV	IPFLTESYSS	SQDPPEKSIP	ICTLKNFPNA	IEHTLQWARD	EFEGLFKQPA	ENVNQYLTDP	KFVERTLRLA	GTQPLEVLEA	VQRSLVLQRP
701	711	721	731	741	751	761	771	781	791
QTWADCVTWA	CHHWHTQYSN	NIRQLLHNFP	PDQLTSSGAP	FWSGPKRCPH	PLTFDVNNPL	HLDYVMAAAN	LFAQTYGLTG	SQDRAAVATF	LQSVQVPEFT
801	811	821	831	841	851	861	871	881	891
PKSGVKIHVS	DQELQSANAS	VDDSRLEELK	ATLPSPDKLP	GFKMYPIDFE	KDDDSNFHMD	FIVAASNLRA	ENYDIPSADR	HKSKLIAGKI	IPAIATTTAA
901	911	921	931	941	951	961	971	981	991
VVGLVCLELY	KVVQGHRQLD	SYKNGFLNLA	LPFFGFSEPL	AAPRHQYYNQ	EWTLWDRFEV	QGLQPNGEEM	TLKQFLDYFK	TEHKLEITML	SQGVSMLYSF
1001	1011	1021	1031	1041	1051
FMPAAKLKER	LDQPMTEIVS	RVSKRKLGRH	VRALVLELCC	NDESGEDVEV	PYVRYTIR

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
40	Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.