Contains      

UniProtKB-P22413

Protein Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Gene ENPP1
Status Reviewed
Source Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver)Jurkat T cell line; HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); Human; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Ovarian tumorJurkat T cell line; Pancreatic islets; Prostate; Prostate tumor; ovarian tumor
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
179 SLCACSDDCKDKGDCCInYSSVCQGEK 2014(33);
285 DPKMnASF 2014(33);
285 MnASFSLK 2011(57); 2012(48); 2012(52); 2013(34); 2014(13); 2014(14); 2014(22); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015;2007;2007;
285 PKMnASFSLK 2012(48);
285 MYDPKMnASFSLK 2014(33);
285 SIVTGLYPESHGIIDNKMYDPKMnASF 2014(33);
341 KMYnGSVPF 2014(33);
341 MYnGSVPFEER 2009(64); 2011(54); 2011(57); 2012(48); 2012(52); 2013(40); 2014(13); 2014(14); 2014(16); 2014(22); 2014(32); 2014(32); 2014(33); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015;2007;2007;
341 MYnGSVPFEERI 2009(64);
341 DIYKMYnGSVPFE 2013(37);
341 KMYnGSVPFEERIL 2014(33);
477 NYEGIARnL 2014(33);
477 SFNYEGIARnL 2014(33);
477 YSFNYEGIARnL 2014(33);
477 nLSCREPNQHFKPYLK 2014(33);
578 nLTPAPNNGTHGSL 2014(33);
578 nLTPAPNNGTHGSLN 2014(33);
578 nLTPAPNNGTHGSLNH 2014(33);
578 nLTPAPNNGTHGSLNHL 2014(33);
578 nLTPAPNNGTHGSLNHLL 2014(33);
578 LLnLTPAPNNGTHGSLNHLLK 2014(33);
578 VYNLMCDLLnLTPAPNNGTHGSLNHLLK 2014(33);
585 NLTPAPNnGTHGSL 2014(33);
585 NLTPAPNnGTHGSLN 2014(33);
585 NLTPAPNnGTHGSLNH 2014(33);
585 NLTPAPNnGTHGSLNHL 2014(33);
585 LTPAPNnGTHGSLNHLLK 2015(unpublished);
585 NLTPAPNnGTHGSLNHLL 2014(33);
585 LLNLTPAPNnGTHGSLNHLLK 2014(33);
585 VYNLMCDLLNLTPAPNnGTHGSLNHLLK 2014(33);
643 nLTVAEEK 2014(33); 2015(1);
643 nLTVAEEKIIKHE 2014(33);
643 nLTVAEEKIIKHETLPY 2014(33);
643 DNLGCSCNPSILPIEDFQTQFnLTVAEEK 2014(33); 2015(unpublished);
700 TVDRnDSFSTE 2014(33);
700 TVDRnDSFSTEDF 2014(33);
700 TVDRnDSFSTEDFSNC 2014(33);
700 TVDRnDSFSTEDFSNCLY 2014(33);
700 TVDRnDSFSTEDFSNCLYQDF 2014(33);
700 TVDRnDSFSTEDFSNCLYQDFR 2014(33); 2015(unpublished);
700 TVDRnDSFSTEDFSNCLYQDFRIPL 2014(33);
731 KnNTKVSY 2014(33);
731 CSFYKnNTK 2014(16);
731 YKnNTKVSY 2014(33);
748 SPPQLNKnSSGIY 2014(33);
748 LSPPQLNKnSSGIY 2014(33);
748 GFLSPPQLNKnSSGIY 2014(33);
748 GFLSPPQLNKnSSGIYSEAL 2015(6);

Sequence

1112131415161718191
MERDGCAGGGSRGGEGGRAPREGPAGNGRDRGRSHAAEAPGDPQAAASLLAPMDVGEEPLEKAARARTAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPS
101111121131141151161171181191
CAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINE
201211221231241251261271281291
PQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEW
301311321331341351361371381391
YKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMV
401411421431441451461471481491
GMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLH
501511521531541551561571581591
FAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVY
601611621631641651661671681691
TPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRN
701711721731741751761771781791
DSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFD
801811821831841851861871881891
FDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSF
901911921
YQQRKEPVSDILKLKTHLPTFSQED

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
6Kim DS, Hahn Y: The acquisition of novel N-glycosylation sites in conserved proteins during human evolution. Bmc Bioinformatics 2015, 16.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.