Contains      

UniProtKB-P27487

Protein Dipeptidyl peptidase 4
Gene DPP4
Status Reviewed
Source ARO Cell Line (Thyroid Cance); Breast cancer xenografts; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCCLM3 cell line (Liver); HepG2 cell line (Liver); HuH-7 cell line (Liver); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Plasma; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Saliva; Serum; Serum (HCC); Spermatozoa; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); plasma
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
85 YGnSSVFLE 2014(33);
85 NAEYGnSSVF 2014(33);
85 NAEYGnSSVFLE 2014(33);
85 VFNAEYGnSSVF 2014(33);
85 YGnSSVFLENSTFDE 2014(33);
85 NAEYGnSSVFLENSTFDE 2014(33);
92 LEnSTFDEF 2014(33);
92 LEnSTFDEFGH 2014(33);
92 LEnSTFDEFGHS 2009(64);
92 YGNSSVFLEnSTFDE 2014(33);
92 LEnSTFDEFGHSINDY 2014(33);
92 NAEYGNSSVFLEnSTFDE 2014(33);
150 IPnNTQWVTW 2014(33);
150 RIPnNTQWVTW 2014(33);
150 ITEERIPnNTQW 2014(33);
150 LITEERIPnNTQW 2009(64);
150 QLITEERIPnNTQW 2014(33);
150 ITEERIPnNTQWVTW 2014(33);
150 PnNTQWVTWSPVGHK 2012(48);
150 IPnNTQWVTWSPVGHK 2007(62); 2009(62); 2009(62); 2009(58); 2011(48); 2012(42); 2013(43); 2013(45); 2013(45); 2013(33); 2014(1); 2015(2); 2015(8); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
150 DLNKRQLITEERIPnNTQW 2014(33);
150 QIITEERIPnNTQWVTWSPVGHK 2014(16);
150 QLITEERIPnNTQWVTWSPVGHK 2009(62); 2009(62); 2013(38); 2014(33); 2015(2);
150 RQLITEERIPnNTQWVTWSPVGHK 2013(38); 2013(38);
219 WSPnGTF 2014(33);
219 WSPnGTFL 2014(33);
219 WWSPnGTFL 2014(33);
219 WSPnGTFLAY 2014(33);
219 WWSPnGTFLAY 2014(33);
219 SALWWSPnGTFL 2014(33);
219 SALWWSPnGTFLAY 2014(33);
229 AQFnDTEVPLIE 2014(33);
229 AQFnDTEVPLIEY 2014(33);
229 AYAQFnDTEVPLIE 2014(33);
229 AYAQFnDTEVPLIEY 2014(33);
229 AQFnDTEVPLIEYSFY 2014(33);
229 LAYAQFnDTEVPLIEY 2014(33);
229 AYAQFnDTEVPLIEYSFY 2014(33);
229 AQFnDTEVPLIEYSFYSDESLQYPK 2014(33);
281 SSVTnATSIQITAPASM 2014(33);
281 VVNTDSLSSVTnATSIQ 2014(33);
281 FVVNTDSLSSVTnATSIQ 2014(33);
281 SSVTnATSIQITAPASML 2014(33);
281 VVNTDSLSSVTnATSIQITAPASML 2014(33);
281 VVNTDSLSSVTnATSIQITAPASMLIGDHY 2014(33);
321 RIQnYSVM 2012(48); 2014(33);
321 IQnYSVMDICDYDE 2014(33);
321 RIQnYSVMDICDYDE 2014(33);
321 IQnYSVMDICDYDESSGR 2009(62); 2009(62); 2009(62); 2012(48); 2013(40); 2014(14); 2014(33); 2015(1); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
321 RIQnYSVMDICDYDESSGR 2007(unpublished);2009(62); 2014(14); 2014(16); 2014(32); 2014(32); 2014(33); 2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
520 IILnETK 2014(33);
520 IILnETKF 2009(64); 2014(33);
520 LDFIILnE 2014(33);
520 DFIILnETK 2014(33);
520 IILnETKFW 2014(33);
520 KLDFIILnE 2014(33);
520 DFIILnETKF 2014(33);
520 IILnETKFWY 2014(33);
520 LDFIILnETK 2007(62); 2009(62); 2009(63); 2009(64); 2009(48); 2012(40); 2013(45); 2013(14); 2014(33); 2014(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;
520 DFIILnETKFW 2014(33);
520 KIDFIIInETK 2013(43); 2014(16); 2014(32); 2014(32);
520 KLDFIILnETK 2005(81); 2009(62); 2009(62); 2009(62); 2009(64); 2011(58); 2012(48); 2013(38); 2013(45); 2014(14); 2014(19); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(10);
520 KLDFIILnETKFWYQMILPPHFDK 2015(2);
685 nSTVMSR 2011(56); 2012(48); 2013(40); 2014(33); 2015(unpublished);2007(unpublished);2007;
685 MGLPTPEDNLDHYRnST 2009(64);
685 MGLPTPEDNLDHYRnSTVM 2014(33);
685 YMGIPTPEDNIDHYRnSTVMSR 2013(43); 2014(16);
685 YMGLPTPEDNLDHYRnSTVMSR 2005(81); 2007(unpublished);2013(34); 2014(14); 2014(33); 2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);
685 TERYMGLPTPEDNLDHYRnSTVM 2014(33);
685 MGLPTPEDNLDHYRnSTVMSRAENF 2014(33);

Sequence

1112131415161718191
MKTPWKVLLGLLGAAALVTIITVPVVLLNKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGH
101111121131141151161171181191
SINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITD
201211221231241251261271281291
WVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYL
301311321331341351361371381391
CDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKG
401411421431441451461471481491
TWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSAL
501511521531541551561571581591
DKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGT
601611621631641651661671681691
FEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEY
701711721731741751761
LLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
56Bandhakavi S, Van Riper SK, Tawfik PN, Stone MD, Haddad T, Rhodus NL, Carlis JV, Griffin TJ: Hexapeptide Libraries for Enhanced Protein PTM Identification and Relative Abundance Profiling in Whole Human Saliva. Journal of Proteome Research 2011, 10:1052-1061.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.