Contains      

UniProtKB-P29966

Protein Myristoylated alanine-rich C-kinase substrate
Gene MARCKS
Status Reviewed
Source HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); Liver; OVCAR-3 cell line (Ovarian cancer)
Years 2009-2015

Glycosites

Site Identified Peptides Year(Publication ID)
61 nGSAPAADKEEPAAAGSGAASPSAAEK 2015(1);
61 LQAnGSAPAADKEEPAAAGSGAASPSAAE 2014(33);
61 EELQAnGSAPAADKEEPAAAGSGAASPSAAE 2014(33);
61 EELQAnGSAPAADKEEPAAAGSGAASPSAAEK 2009(63); 2013(40); 2014(33);
61 EELQAnGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEK 2014(33);
148 AEDGATPSPSnETPKK 2014(15);
148 EAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSnETPK 2014(15);
148 EAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSnETPKK 2014(15);

Sequence

1112131415161718191
MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGA
101111121131141151161171181191
SPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGA
201211221231241251261271281291
AAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACEAPSAAGPGAPPEQEAAPAEE
301311321331
PAAAAASSACAAPSQEAQPECSPEAPPAEAAE

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.