Contains      

UniProtKB-P32004

Protein Neural cell adhesion molecule L1
Gene L1CAM
Status Reviewed
Source Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Plasma; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Urine; UrineJurkat T cell line; XTC-1 Cell Line (Thyroid Cance); ovarian tumor
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
100 nNSNFAQR 2015(1);
100 TITGnNSNFAQR 2015(unpublished);2007(unpublished);2007;
100 EELGVTVYQSPHSGSFTITGnNSNF 2007(unpublished);
100 EEIGVTVYQSPHSGSFTITGnNSNFAQR 2014(16);
100 EELGVTVYQSPHSGSFTITGnNSNFAQR 2012(52); 2015(1);
203 FANVLTSDnHSDY 2014(33);
203 nHSDYICHAHFPGTR 2015(1);
203 VTMGQNGNIYFANVITSDnHSDYICHAHFPGTR 2014(16);
203 VTMGQNGNLYFANVLTSDnHSDYICHAHFPGTR 2015(12);
247 LLFPTnSSSHL 2014(33); 2015(unpublished);
247 LLFPTnSSSHLVALQ 2007(75);
247 LLFPTnSSSHLVALQGQPLVLE 2014(33);
247 IIFPTnSSSHIVAIQGQPIVIECIAEGFPTPTIK 2014(16);
247 LLFPTnSSSHLVALQGQPLVLECIAEGFPTPTIK 2007(75); 2009(62); 2011(58); 2012(52); 2013(45); 2014(17); 2015(1); 2015(2); 2015(12); 2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;
294 VTYQNHnK 2013(40); 2015(unpublished);2015(2);
433 nQTYMAVQGSTAYLLCK 2015(1);
433 ILTADnQTYMAVQGSTAY 2007(unpublished);2007(unpublished);2007(unpublished);
433 IITADnQTYMAVQGSTAYIICK 2014(16);
433 ILTADnQTYMAVQGSTAYLLCK 2007(75); 2009(67); 2011(58); 2012(52); 2013(40); 2014(18); 2015(unpublished);2015(1);
479 nGTLGIR 2015(1);
479 FFPYAnGTIGIR 2014(16);
479 FFPYAnGTLGIR 2007(75); 2007(67); 2009(58); 2011(52); 2012(34); 2013(34); 2013(40); 2013(45); 2013(17); 2014(22); 2014(33); 2014(1); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007;2007;2007;2007;2007;
479 RFFPYAnGTIGIR 2013(37);
479 AFGAPVPSVQWLDEDGTTVLQDERFFPYAnGTLGIR 2015(2);
490 DIQAnDTGR 2014(16);
490 DLQAnDTGR 2007(75); 2012(48); 2013(40); 2014(17); 2014(22); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015;2007;2007;
490 IRDIQAnDTGRYF 2013(37);
505 nVTIMANLK 2015(1);
588 nYSCVASTELDVVESR 2015(1);
588 IVIHSIDYSDQGnYSCVASTEIDVVESR 2014(16);
588 LVIHSLDYSDQGnYSCVASTELDVVESR 2012(52); 2013(34); 2015(unpublished);2015(1); 2015(12);
671 nQTSTTLK 2015(1);
671 GnQTSTTLK 2015(unpublished);
671 VPGnQTSTTIK 2014(16);
671 VPGnQTSTTLK 2005( 81); 2007(75); 2007(67); 2009(54); 2011(48); 2012(52); 2012(34); 2013(34); 2013(40); 2013(13); 2014(14); 2014(17); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;
671 LGKVPGnQTSTT 2009(64);
671 IGKVPGnQTSTTI 2013(37);
671 SLGKVPGnQTSTTL 2014(33);
671 YSLGKVPGnQTSTTL 2014(33);
671 WYSLGKVPGnQTSTTLK 2015(12);
726 DVKGEGnETTNMV 2013(37);
726 nETTNMVITWKPLR 2015(1);
726 GEGnETTNMVITWKPIR 2014(16);
726 GEGnETTNMVITWKPLR 2009(67); 2012(52); 2013(40); 2013(45); 2014(22); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(12);
726 YGPGEPSPVSETVVTPEAAPEKNPVDVKGEGnETTNMVITWKPLR 2015(1); 2015(12);
777 nTSTFVPYEIK 2015(1);
777 VVSnTSTFVPYEIK 2007(unpublished);2007(unpublished);2007;
777 GPWQEQIVSDPFLVVSnTSTFVPYEIK 2009(67); 2014(17); 2014(33); 2015(1); 2015(2); 2015(12);
777 VQWRPQGTRGPWQEQIVSDPFLVVSnTSTFVPYEIK 2015(12);
825 nSSAVLVK 2015(1);
825 ILnSSAVLVK 2014(33);
825 LEGIEILnSSAVLVK 2014(33);
825 GPEPQVTIGYSGEDYPQAIPELEGIEILnSSAVLVK 2015(1); 2015(2); 2007(unpublished);2007(unpublished);2007(unpublished);
825 VQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILnSSAVLVK 2015(12);
849 GYnVTYWR 2007(58); 2011(52); 2012(40); 2013(16); 2014(17); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;
849 GHIRGYnVTYWRE 2013(37);
876 DHVVVPAnTTSVILSGLRPY 2007(33); 2014(unpublished);2015(unpublished);2007(unpublished);2007;2007;
876 DHVVVPAnTTSVILSGLRPYSSYHLEVQAFNGR 2015(unpublished);2015(1);
931 GSGPASEFTFSTPEGVPGHPEAIHIECQSnTSIIIR 2014(16);
931 GSGPASEFTFSTPEGVPGHPEALHLECQSnTSLLLR 2007(unpublished);2012(52); 2013(34); 2014(17); 2015(unpublished);2015(1); 2015(12);
979 nLTDLSPHLR 2015(1);
979 THnLTDLSPH 2015(unpublished);
979 HnLTDLSPHLR 2007(unpublished);
979 THnITDISPHIR 2014(16);
979 THnLTDLSPHLR 2009(67); 2011(58); 2012(52); 2013(34); 2013(34); 2013(42); 2013(45); 2014(13); 2014(17); 2014(18); 2014(22); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;2007;
979 PEIRTHnITDISP 2013(37);
1022 nISATAGE 2015(1);
1022 EGGTMALSGISDFGnISATAGENYSVVSWVPK 2015(1);
1030 EGGTMALSGISDFGNISATAGEnYSVVSWVPK 2015(1);
1071 GGASLSPQYVSYnQSSYTQWDLQPDTDYEIHLFK 2015(unpublished);

Sequence

1112131415161718191
MVVALRYVWPLLLCSPCLLIQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGN
101111121131141151161171181191
NSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLT
201211221231241251261271281291
SDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLL
301311321331341351361371381391
KVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTM
401411421431441451461471481491
VTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAA
501511521531541551561571581591
NDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVV
601611621631641651661671681691
ESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEP
701711721731741751761771781791
SPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEP
801811821831841851861871881891
QVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNG
901911921931941951961971981991
RGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEG
1001101110211031104110511061107110811091
PGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQM
1101111111211131114111511161117111811191
AVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIK
120112111221123112411251
PLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.