Contains      

UniProtKB-P34810

Protein Macrosialin
Gene CD68
Status Reviewed
Source Breast cancer cell lines; Breast cell lines; Breast tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Platelet; Platelet Plasma membranes; Prostate; Prostate tumor; Spermatozoa; Urine
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
164 TnGSQPCVHL 2014(33);
164 TWTnGSQPCVH 2014(33);
164 TWTnGSQPCVHL 2014(33);
164 ETIGDYTWTnGSQPCVH 2012(3); 2007(unpublished);2007(unpublished);
164 ETIGDYTWTnGSQPCVHLQAQIQIR 2012(52); 2014(33); 2015(unpublished);2015(unpublished);
199 ISVLNPnKTK 2009(64);
199 AWGISVLNPnK 2014(33);
199 GISVLNPnKTK 2014(33);
199 AWGISVLNPnKTK 2014(33);
199 NPnKTKVQGSCEGAHPH 2014(33);
199 TTQGGGEAWGISVLNPnK 2014(33);
199 TTQGGGEAWGISVLNPnKTK 2014(33);
199 VMYTTQGGGEAWGISVLNPnK 2007(74); 2012(52); 2013(45);
199 GISVLNPnKTKVQGSCEGAHPH 2014(33);
199 VMYTTQGGGEAWGISVINPnKTK 2014(16);
199 VMYTTQGGGEAWGISVLNPnKTK 2012(52); 2013(34); 2014(33); 2015(unpublished);2015(2);
199 GISVLNPnKTKVQGSCEGAHPHLL 2014(33);
199 GISVLNPnKTKVQGSCEGAHPHLLL 2014(33);
246 YnVSFPH 2014(33);
246 nVSFPHAAQW 2014(33);
246 MAVEYnVSFPH 2014(33);
246 YnVSFPHAAQW 2014(33);
246 MAVEYnVSFPHA 2014(33);
246 MAVEYnVSFPHAA 2014(33);
246 VEYnVSFPHAAQW 2014(33);
246 AVEYnVSFPHAAQW 2014(33);
246 MAVEYnVSFPHAAQW 2014(33);
246 nVSFPHAAQWTFSAQ 2015(1);
246 SYMAVEYnVSFPHAAQW 2014(33);
246 LSYMAVEYnVSFPHAAQW 2014(33);
246 AVEYnVSFPHAAQWTFSAQNASLR 2015(unpublished);
246 MAVEYnVSFPHAAQWTFSAQNASLR 2015(unpublished);
261 SAQnASLR 2014(33); 2015(unpublished);
261 SAQnASLRDL 2014(33);
261 TFSAQnASLR 2014(33); 2015(unpublished);2007;
261 SAQnASLRDLQ 2014(33);
261 WTFSAQnASLR 2007(75); 2009(64); 2012(48); 2014(33); 2015(unpublished);
261 TFSAQnASLRDL 2014(33);
261 SAQnASLRDLQAP 2014(33);
261 AAQWTFSAQnASLR 2015(unpublished);
261 SAQnASLRDLQAPL 2014(33);
261 SAQnASLRDLQAPLG 2014(33);
261 SAQnASLRDLQAPLGQ 2014(33);
261 TFSAQnASLRDLQAPL 2014(33);
261 SAQnASLRDLQAPLGQSF 2014(33);
261 TFSAQnASLRDLQAPLGQ 2014(33);
261 TFSAQnASLRDLQAPLGQSF 2014(33);
261 AVEYNVSFPHAAQWTFSAQnASLR 2015(unpublished);
261 MAVEYNVSFPHAAQWTFSAQnASLR 2015(unpublished);
279 SnSSIILSPAVHL 2014(33);
279 SnSSIILSPAVHLD 2014(33);
279 SCSnSSIILSPAVHL 2014(33);
279 SCSnSSIILSPAVHLD 2014(33);
279 DLQAPLGQSFSCSnSSII 2014(14); 2015(unpublished);
279 SCSnSSIILSPAVHLDLL 2014(33);
279 SnSSIILSPAVHLDLLSLR 2015(unpublished);
279 SCSnSSIILSPAVHLDLLSL 2014(33);
279 SCSnSSIILSPAVHLDLLSLR 2015(unpublished);
279 SFSCSnSSIILSPAVHLDLLSLR 2015(unpublished);
279 DLQAPLGQSFSCSnSSIILSPAVHLD 2014(33);
279 DLQAPLGQSFSCSnSSIILSPAVHLDL 2014(14); 2015(unpublished);
279 DIQAPIGQSFSCSnSSIIISPAVHIDIISIR 2013(43); 2014(16);
279 DLQAPLGQSFSCSnSSIILSPAVHLDLLSLR 2009(64); 2012(52); 2014(14); 2014(25); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;
279 SCSnSSIILSPAVHLDLLSLRLQAAQLPHTGVF 2014(33);

Sequence

1112131415161718191
MRLAVLFSGALLGLLAAQGTGNDCPHKKSATLLPSFTVTPTVTESTGTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTVHPTSNSTAT
101111121131141151161171181191
SQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPSPSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPNK
201211221231241251261271281291
TKVQGSCEGAHPHLLLSFPYGHLSFGFMQDLQQKVVYLSYMAVEYNVSFPHAAQWTFSAQNASLRDLQAPLGQSFSCSNSSIILSPAVHLDLLSLRLQAA
301311321331341351
QLPHTGVFGQSFSCPSDRSILLPLIIGLILLGLLALVLIAFCIIRRRPSAYQAL

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.