Detail


Protein	Glycogen debranching enzyme
Gene	AGL
Status	Reviewed
Source	Liver; OVCAR-3 cell line (Ovarian cancer); Plasma
Years	2005-2015

Site	Identified Peptides	Year(Publication ID)
797	KDENSInGTPDITVE	2014(33);
797	DENSInGTPDITVEIR	2005(81);
797	KDENSInGTPDITVEIR	2014(33);
1032	nGSTFVK	2015(1);

Sequence

1	11	21	31	41	51	61	71	81	91
MGHSKQIRIL	LLNEMEKLEK	TLFRLEQGYE	LQFRLGPTLQ	GKAVTVYTNY	PFPGETFNRE	KFRSLDWENP	TEREDDSDKY	CKLNLQQSGS	FQYYFLQGNE
101	111	121	131	141	151	161	171	181	191
KSGGGYIVVD	PILRVGADNH	VLPLDCVTLQ	TFLAKCLGPF	DEWESRLRVA	KESGYNMIHF	TPLQTLGLSR	SCYSLANQLE	LNPDFSRPNR	KYTWNDVGQL
201	211	221	231	241	251	261	271	281	291
VEKLKKEWNV	ICITDVVYNH	TAANSKWIQE	HPECAYNLVN	SPHLKPAWVL	DRALWRFSCD	VAEGKYKEKG	IPALIENDHH	MNSIRKIIWE	DIFPKLKLWE
301	311	321	331	341	351	361	371	381	391
FFQVDVNKAV	EQFRRLLTQE	NRRVTKSDPN	QHLTIIQDPE	YRRFGCTVDM	NIALTTFIPH	DKGPAAIEEC	CNWFHKRMEE	LNSEKHRLIN	YHQEQAVNCL
401	411	421	431	441	451	461	471	481	491
LGNVFYERLA	GHGPKLGPVT	RKHPLVTRYF	TFPFEEIDFS	MEESMIHLPN	KACFLMAHNG	WVMGDDPLRN	FAEPGSEVYL	RRELICWGDS	VKLRYGNKPE
501	511	521	531	541	551	561	571	581	591
DCPYLWAHMK	KYTEITATYF	QGVRLDNCHS	TPLHVAEYML	DAARNLQPNL	YVVAELFTGS	EDLDNVFVTR	LGISSLIREA	MSAYNSHEEG	RLVYRYGGEP
601	611	621	631	641	651	661	671	681	691
VGSFVQPCLR	PLMPAIAHAL	FMDITHDNEC	PIVHRSAYDA	LPSTTIVSMA	CCASGSTRGY	DELVPHQISV	VSEERFYTKW	NPEALPSNTG	EVNFQSGIIA
701	711	721	731	741	751	761	771	781	791
ARCAISKLHQ	ELGAKGFIQV	YVDQVDEDIV	AVTRHSPSIH	QSVVAVSRTA	FRNPKTSFYS	KEVPQMCIPG	KIEEVVLEAR	TIERNTKPYR	KDENSINGTP
801	811	821	831	841	851	861	871	881	891
DITVEIREHI	QLNESKIVKQ	AGVATKGPNE	YIQEIEFENL	SPGSVIIFRV	SLDPHAQVAV	GILRNHLTQF	SPHFKSGSLA	VDNADPILKI	PFASLASRLT
901	911	921	931	941	951	961	971	981	991
LAELNQILYR	CESEEKEDGG	GCYDIPNWSA	LKYAGLQGLM	SVLAEIRPKN	DLGHPFCNNL	RSGDWMIDYV	SNRLISRSGT	IAEVGKWLQA	MFFYLKQIPR
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
YLIPCYFDAI	LIGAYTTLLD	TAWKQMSSFV	QNGSTFVKHL	SLGSVQLCGV	GKFPSLPILS	PALMDVPYRL	NEITKEKEQC	CVSLAAGLPH	FSSGIFRCWG
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
RDTFIALRGI	LLITGRYVEA	RNIILAFAGT	LRHGLIPNLL	GEGIYARYNC	RDAVWWWLQC	IQDYCKMVPN	GLDILKCPVS	RMYPTDDSAP	LPAGTLDQPL
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
FEVIQEAMQK	HMQGIQFRER	NAGPQIDRNM	KDEGFNITAG	VDEETGFVYG	GNRFNCGTWM	DKMGESDRAR	NRGIPATPRD	GSAVEIVGLS	KSAVRWLLEL
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
SKKNIFPYHE	VTVKRHGKAI	KVSYDEWNRK	IQDNFEKLFH	VSEDPSDLNE	KHPNLVHKRG	IYKDSYGASS	PWCDYQLRPN	FTIAMVVAPE	LFTTEKAWKA
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
LEIAEKKLLG	PLGMKTLDPD	DMVYCGIYDN	ALDNDNYNLA	KGFNYHQGPE	WLWPIGYFLR	AKLYFSRLMG	PETTAKTIVL	VKNVLSRHYV	HLERSPWKGL
1501	1511	1521	1531
PELTNENAQY	CPFSCETQAW	SIATILETLY	DL

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
81	Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.

UniProtKB-P35573

Glycosites

Sequence

Reference