Contains      

UniProtKB-P42892

Protein Endothelin-converting enzyme 1
Gene ECE1
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HepG2 cell line (Liver); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; Lymphocytes; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Platelet; Platelet Plasma membranes; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; TPC-1 Cell Line (Thyroid Cance); Urine; prostate cancer cell lines
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
166 nSTASVSEAER 2014(33); 2015(1);
166 HLLEnSTASVSE 2014(33);
166 nSTASVSEAERK 2015(1);
166 IKHIIEnSTASVS 2013(37);
166 HLLEnSTASVSEAER 2007(72); 2007(74); 2007(75); 2007(62); 2009(62); 2009(64); 2009(67); 2009(57); 2011(48); 2012(50); 2012(52); 2012(34); 2013(34); 2013(40); 2013(13); 2014(14); 2014(15); 2014(17); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;2007;2007;
166 HIIEnSTASVSEAERK 2014(16);
166 HLLEnSTASVSEAERK 2007(72); 2007(75); 2009(64); 2011(57); 2011(58); 2012(52); 2013(34); 2013(34); 2013(38); 2013(38); 2014(13); 2014(14); 2014(15); 2014(18); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(9); 2015(12);
187 CMnETR 2012(48);
187 ACMnETR 2012(48); 2013(40); 2014(14); 2014(22); 2014(33); 2015(unpublished);
187 RACMnETR 2012(48);
187 ACMnETRIEEIR 2014(16);
187 ACMnETRIEELR 2011(57); 2011(58); 2012(48); 2014(14); 2014(17); 2015(unpublished);2015(12);
187 RACMnETRIEEL 2014(33);
210 nITGPWAK 2015(1);
210 WnITGPWAK 2012(48);
210 GWnITGPWAK 2012(48);
210 GGWnITGPWAK 2012(48);
210 IGGWnITGPWAK 2014(16);
210 LGGWnITGPWAK 2007(72); 2007(74); 2007(75); 2009(62); 2009(63); 2009(64); 2011(57); 2012(48); 2012(52); 2013(40); 2014(15); 2014(17); 2014(22); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;
210 RLGGWnITGPWAKDNF 2009(64);
210 IERLGGWnITGPWAKDNF 2009(64);
210 LGGWnITGPWAKDNFQDTLQVVTAHYR 2011(58); 2015(12);
270 DYYInK 2014(32);
270 DYYLnK 2012(48); 2013(40); 2014(14); 2014(22); 2014(33); 2015(2); 2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;
270 DYYLnKTE 2014(33);
270 YLnKTENEK 2012(48);
270 LnKTENEKVL 2014(33);
270 YYLnKTENEK 2012(48);
270 DYYInKTENEK 2014(16);
270 DYYLnKTENEK 2007(72); 2007(75); 2009(64); 2012(48); 2012(52); 2013(34); 2013(34); 2014(13); 2014(14); 2014(17); 2014(22); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(12);
270 YLnKTENEKVL 2014(33);
270 NSNSNVIQVDQSGLGLPSRDYYLnK 2011(57);
270 NSNSNVIQVDQSGIGIPSRDYYInKTENEK 2014(16);
270 NSNSNVIQVDQSGLGLPSRDYYLnKTENEK 2015(12);
316 nITIPQEK 2012(48); 2015(1);
316 AnITIPQEK 2012(48);
316 LAnITIPQEK 2012(48);
316 TALAnITIPQEK 2012(48); 2014(33);
316 TALAnITIPQEKR 2014(33);
316 DFETALAnITIPQEK 2012(48); 2014(33);
316 LDFETALAnITIPQEK 2012(48);
316 ILDFETALAnITIPQEK 2011(57); 2012(48);
316 QILDFETALAnITIPQEK 2012(48);
316 MQQILDFETALAnITIPQEK 2012(48);
316 DFETALAnITIPQEKRRDEEL 2014(33);
316 PQMQQILDFETALAnITIPQEK 2012(48);
316 AIRPQMQQILDFETALAnITIPQEK 2012(48);
316 LLGGGDEEAIRPQMQQILDFETALAnITIPQEK 2007(75); 2009(64); 2011(57); 2011(58); 2012(52); 2014(14); 2014(17); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;2007;
316 IIGGGDEEAIRPQMQQIIDFETAIAnITIPQEKR 2014(16);
316 LLGGGDEEAIRPQMQQILDFETALAnITIPQEKR 2015(12);
362 nESEPIVVYDK 2012(48);
362 InESEPIVVYDK 2012(48);
362 LNTIFYPVEInE 2014(33);
362 EInESEPIVVYDK 2012(48);
362 TIFYPVEInESEPIVVYDK 2012(48); 2015(unpublished);2007(unpublished);2007;
362 NTIFYPVEInESEPIVVYDK 2012(48);
362 LNTIFYPVEInESEPIVVYDK 2012(48); 2014(33);
383 InTTDRCLL 2014(33);
383 LInTTDRCLL 2014(33);
383 YLEQISTLInTTDR 2014(33);
383 EYIEQISTIInTTDR 2014(16);
383 EYLEQISTLInTTDR 2009(67); 2011(57); 2012(48); 2012(52); 2013(40); 2014(22); 2014(33); 2015(1); 2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;
539 FFnFSWR 2007(72); 2007(74); 2012(48); 2013(40); 2014(16); 2014(17); 2014(33); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
632 nSSVEAFK 2007(74); 2012(48); 2014(14); 2014(22); 2015(unpublished);2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(12);
632 nSSVEAFKR 2007(72); 2013(34); 2014(13); 2014(14); 2014(33); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(12);
632 WKnSSVEAF 2014(33);
651 nYSVNGEPVNGR 2012(48);
651 SnYSVNGEPVNGR 2012(48);
651 YSnYSVNGEPVNGR 2012(48);
651 VEQYSnYSVNGEPVNGR 2007(unpublished);2007(unpublished);2007(unpublished);
651 MVEQYSnYSVNGEPVNGR 2011(57);
651 QTECMVEQYSnYSVNGEPVNGR 2012(52); 2014(33); 2015(unpublished);2015(12);
651 RQTECMVEQYSnYSVNGEPVNGR 2011(57);
651 RQTECMVEQYSnYSVNGEPVNGRHTLGENIADNGGLK 2013(38);

Sequence

1112131415161718191
MRGVWPPPVSALLSALGMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCL
101111121131141151161171181191
SEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLM
201211221231241251261271281291
ELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAI
301311321331341351361371381391
RPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVR
401411421431441451461471481491
KTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAI
501511521531541551561571581591
YNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGG
601611621631641651661671681691
IGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGL
701711721731741751761
TNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.