Detail

UniProtKB-P46531


Protein	Neurogenic locus notch homolog protein 1
Gene	NOTCH1
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; OVCAR-3 cell line (Ovarian cancer)
Years	2011-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
41	nGTEACVCGGAFVGPR	2015(1);
1241	CFNnGTCVDQVGGYSCTCPPGFVGER	2012(52);
1489	nCTQSIQCWK	2014(16);
1489	nCTQSLQCWK	2011(58); 2012(52); 2014(13); 2014(33); 2015(1); 2015(unpublished);
1489	FNDPWKnCTQSIQ	2013(37);
1587	nSSFHFLR	2011(58); 2014(33); 2015(2); 2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MPPLLAPLLC	LALLPALAAR	GPRCSQPGET	CLNGGKCEAA	NGTEACVCGG	AFVGPRCQDP	NPCLSTPCKN	AGTCHVVDRR	GVADYACSCA	LGFSGPLCLT
101	111	121	131	141	151	161	171	181	191
PLDNACLTNP	CRNGGTCDLL	TLTEYKCRCP	PGWSGKSCQQ	ADPCASNPCA	NGGQCLPFEA	SYICHCPPSF	HGPTCRQDVN	ECGQKPGLCR	HGGTCHNEVG
201	211	221	231	241	251	261	271	281	291
SYRCVCRATH	TGPNCERPYV	PCSPSPCQNG	GTCRPTGDVT	HECACLPGFT	GQNCEENIDD	CPGNNCKNGG	ACVDGVNTYN	CRCPPEWTGQ	YCTEDVDECQ
301	311	321	331	341	351	361	371	381	391
LMPNACQNGG	TCHNTHGGYN	CVCVNGWTGE	DCSENIDDCA	SAACFHGATC	HDRVASFYCE	CPHGRTGLLC	HLNDACISNP	CNEGSNCDTN	PVNGKAICTC
401	411	421	431	441	451	461	471	481	491
PSGYTGPACS	QDVDECSLGA	NPCEHAGKCI	NTLGSFECQC	LQGYTGPRCE	IDVNECVSNP	CQNDATCLDQ	IGEFQCICMP	GYEGVHCEVN	TDECASSPCL
501	511	521	531	541	551	561	571	581	591
HNGRCLDKIN	EFQCECPTGF	TGHLCQYDVD	ECASTPCKNG	AKCLDGPNTY	TCVCTEGYTG	THCEVDIDEC	DPDPCHYGSC	KDGVATFTCL	CRPGYTGHHC
601	611	621	631	641	651	661	671	681	691
ETNINECSSQ	PCRHGGTCQD	RDNAYLCFCL	KGTTGPNCEI	NLDDCASSPC	DSGTCLDKID	GYECACEPGY	TGSMCNINID	ECAGNPCHNG	GTCEDGINGF
701	711	721	731	741	751	761	771	781	791
TCRCPEGYHD	PTCLSEVNEC	NSNPCVHGAC	RDSLNGYKCD	CDPGWSGTNC	DINNNECESN	PCVNGGTCKD	MTSGYVCTCR	EGFSGPNCQT	NINECASNPC
801	811	821	831	841	851	861	871	881	891
LNQGTCIDDV	AGYKCNCLLP	YTGATCEVVL	APCAPSPCRN	GGECRQSEDY	ESFSCVCPTG	WQGQTCEVDI	NECVLSPCRH	GASCQNTHGG	YRCHCQAGYS
901	911	921	931	941	951	961	971	981	991
GRNCETDIDD	CRPNPCHNGG	SCTDGINTAF	CDCLPGFRGT	FCEEDINECA	SDPCRNGANC	TDCVDSYTCT	CPAGFSGIHC	ENNTPDCTES	SCFNGGTCVD
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
GINSFTCLCP	PGFTGSYCQH	DVNECDSQPC	LHGGTCQDGC	GSYRCTCPQG	YTGPNCQNLV	HWCDSSPCKN	GGKCWQTHTQ	YRCECPSGWT	GLYCDVPSVS
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
CEVAAQRQGV	DVARLCQHGG	LCVDAGNTHH	CRCQAGYTGS	YCEDLVDECS	PSPCQNGATC	TDYLGGYSCK	CVAGYHGVNC	SEEIDECLSH	PCQNGGTCLD
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
LPNTYKCSCP	RGTQGVHCEI	NVDDCNPPVD	PVSRSPKCFN	NGTCVDQVGG	YSCTCPPGFV	GERCEGDVNE	CLSNPCDARG	TQNCVQRVND	FHCECRAGHT
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
GRRCESVING	CKGKPCKNGG	TCAVASNTAR	GFICKCPAGF	EGATCENDAR	TCGSLRCLNG	GTCISGPRSP	TCLCLGPFTG	PECQFPASSP	CLGGNPCYNQ
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
GTCEPTSESP	FYRCLCPAKF	NGLLCHILDY	SFGGGAGRDI	PPPLIEEACE	LPECQEDAGN	KVCSLQCNNH	ACGWDGGDCS	LNFNDPWKNC	TQSLQCWKYF
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
SDGHCDSQCN	SAGCLFDGFD	CQRAEGQCNP	LYDQYCKDHF	SDGHCDQGCN	SAECEWDGLD	CAEHVPERLA	AGTLVVVVLM	PPEQLRNSSF	HFLRELSRVL
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
HTNVVFKRDA	HGQQMIFPYY	GREEELRKHP	IKRAAEGWAA	PDALLGQVKA	SLLPGGSEGG	RRRRELDPMD	VRGSIVYLEI	DNRQCVQASS	QCFQSATDVA
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
AFLGALASLG	SLNIPYKIEA	VQSETVEPPP	PAQLHFMYVA	AAAFVLLFFV	GCGVLLSRKR	RRQHGQLWFP	EGFKVSEASK	KKRREPLGED	SVGLKPLKNA
1801	1811	1821	1831	1841	1851	1861	1871	1881	1891
SDGALMDDNQ	NEWGDEDLET	KKFRFEEPVV	LPDLDDQTDH	RQWTQQHLDA	ADLRMSAMAP	TPPQGEVDAD	CMDVNVRGPD	GFTPLMIASC	SGGGLETGNS
1901	1911	1921	1931	1941	1951	1961	1971	1981	1991
EEEEDAPAVI	SDFIYQGASL	HNQTDRTGET	ALHLAARYSR	SDAAKRLLEA	SADANIQDNM	GRTPLHAAVS	ADAQGVFQIL	IRNRATDLDA	RMHDGTTPLI
2001	2011	2021	2031	2041	2051	2061	2071	2081	2091
LAARLAVEGM	LEDLINSHAD	VNAVDDLGKS	ALHWAAAVNN	VDAAVVLLKN	GANKDMQNNR	EETPLFLAAR	EGSYETAKVL	LDHFANRDIT	DHMDRLPRDI
2101	2111	2121	2131	2141	2151	2161	2171	2181	2191
AQERMHHDIV	RLLDEYNLVR	SPQLHGAPLG	GTPTLSPPLC	SPNGYLGSLK	PGVQGKKVRK	PSSKGLACGS	KEAKDLKARR	KKSQDGKGCL	LDSSGMLSPV
2201	2211	2221	2231	2241	2251	2261	2271	2281	2291
DSLESPHGYL	SDVASPPLLP	SPFQQSPSVP	LNHLPGMPDT	HLGIGHLNVA	AKPEMAALGG	GGRLAFETGP	PRLSHLPVAS	GTSTVLGSSS	GGALNFTVGG
2301	2311	2321	2331	2341	2351	2361	2371	2381	2391
STSLNGQCEW	LSRLQSGMVP	NQYNPLRGSV	APGPLSTQAP	SLQHGMVGPL	HSSLAASALS	QMMSYQGLPS	TRLATQPHLV	QTQQVQPQNL	QMQQQNLQPA
2401	2411	2421	2431	2441	2451	2461	2471	2481	2491
NIQQQQSLQP	PPPPPQPHLG	VSSAASGHLG	RSFLSGEPSQ	ADVQPLGPSS	LAVHTILPQE	SPALPTSLPS	SLVPPVTAAQ	FLTPPSQHSY	SSPVDNTPSH
2501	2511	2521	2531	2541	2551
QLQVPEHPFL	TPSPESPDQW	SSSSPHSNVS	DWSEGVSSPP	TSMQSQIARI	PEAFK

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
13	Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
52	Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58	Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.