Contains      

UniProtKB-P48357

Protein Leptin receptor
Gene LEPR
Status Reviewed
Source HuH-7 cell line (Liver); K562A cell line (Leukemia); Liver; Ovarian tumor; Plasma; Prostate; Prostate tumor; Serum; Serum (HCC); Urine
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
23 FnLSYPITPW 2014(33);
23 FnLSYPITPWR 2014(33);
41 SCMPPnSTYDYF 2014(33);
41 LSCMPPnSTYDYF 2014(33);
41 LSCMPPnSTYDYFLLPAGLSK 2014(33);
56 nTSNSNGHYE 2014(33);
56 nTSNSNGHYETAVEPK 2013(45); 2014(33); 2015(unpublished);2015(unpublished);
56 LLPAGLSKnTSNSNGHY 2014(33);
73 FnSSGTHF 2014(33);
73 FnSSGTHFSNLSK 2007(38); 2013(45); 2013(33); 2014(unpublished);2015(unpublished);2015;2015;2007;2007;
73 ETAVEPKFnSSGTHF 2014(33);
81 SnLSKTTF 2014(33);
81 FNSSGTHFSnLSK 2007(38); 2013(45); 2013(33); 2014(unpublished);2015(unpublished);2015;2015;2007;2007;
98 nCSLCADNIEGK 2007(unpublished);2007(unpublished);
98 SEQDRnCSLCADNIEGK 2013(45); 2014(33); 2015(unpublished);2015(unpublished);
187 GSFQMVHCnCSVHE 2014(33);
187 GSFQMVHCnCSVHECCEC 2007(unpublished);2007(unpublished);
187 GSFQMVHCnCSVHECCECLVPVPTAK 2014(33);
206 LnDTLLMCLK 2014(33);
276 YSEnSTTVIR 2005( 81); 2009(64); 2013(38); 2014(14); 2014(25); 2014(33); 2015(unpublished);2015(unpublished);2007;2007;
276 YSEnSTTVIRE 2014(33);
347 TSVGSnVSFH 2014(33);
347 ILTSVGSnVSFH 2014(33);
347 ILTSVGSnVSFHCIY 2014(33);
347 ILTSVGSnVSFHCIYK 2014(33);
397 NLnETKPR 2014(33);
397 VTFFNLnE 2014(33);
397 FNLnETKPR 2014(33);
397 FNLnETKPRGK 2009(64);
397 NLnETKPRGKF 2014(33);
397 FNLnETKPRGKF 2014(33);
397 VTFFNLnETKPR 2009(64); 2014(33);
516 InHSLGSL 2014(33);
516 GYTMWIRInHSLGSLDSPPTCVLPDSVVK 2005(81);
516 InHSLGSLDSPPTCVLPDSVVKPLPPSSVK 2014(33);
624 LDGLGYWSnWSNPAYTVVMDIK 2014(33);
659 KKEKnVTLL 2014(33);
659 nVTLLWKPLMK 2014(33);
659 EKnVTLLWKPLMK 2014(33);
670 nDSLCSVQR 2014(33);
688 TSCnGTWSEDVGNHTK 2014(33);
688 YVINHHTSCnGTWSEDVGNHTK 2014(33);
697 TSCNGTWSEDVGnHTK 2014(33);
697 YVINHHTSCNGTWSEDVGnHTK 2014(33);
728 nLTFSWPMSK 2014(33);
750 SAYPLnSSCVIVSW 2014(33);

Sequence

1112131415161718191
MICQKFCVVLLHWEFIYVITAFNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCS
101111121131141151161171181191
LCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPV
201211221231241251261271281291
PTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILP
301311321331341351361371381391
GSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETK
401411421431441451461471481491
PRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIF
501511521531541551561571581591
QPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPV
601611621631641651661671681691
PDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTK
701711721731741751761771781791
FTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDH
801811821831841851861871881891
FIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDAGLYVIVPVIISSSILLLGTLLISHQRMKKLFWEDVPNPKNCSWAQGLNFQKPETFEHLFI
901911921931941951961971981991
KHTASVTCGPLLLEPETISEDISVDTSWKNKDEMMPTTVVSLLSTTDLEKGSVCISDQFNSVNFSEAEGTEVTYEDESQRQPFVKYATLISNSKPSETGE
1001101110211031104110511061107110811091
EQGLINSSVTKCFSSKNSPLKDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYLGVTSIKKRESGVLLTDKSRV
1101111111211131114111511161
SCPFPAPCLFTDIRVLQDSCSHFVENNINLGTSSKKTFASYMPQFQTCSTQTHKIMENKMCDLTV

Reference

ID Publication
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.