Detail

UniProtKB-P55011


Protein	Solute carrier family 12 member 2
Gene	SLC12A2
Status	Reviewed
Source	22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Breast cancer xenografts; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); Hela cell line (cervical cancer); Lymphocytes; OVCAR-3 cell line (Ovarian cancer); Pancreatic islets; Prostate tumor; prostate cancer cell lines
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
553	DATGNVnDTIVTEITNCTSAACK	2014(16);
553	DATGNVnDTIVTELTNCTSAACK	2007(72); 2009(62); 2012(50); 2014(22); 2015(1); 2015(12); 2015(unpublished);2007(unpublished);2007;
562	nCTSAACK	2015(1);
562	LTnCTSAACK	2012(48);
562	DATGNVNDTIVTEITnCTSAACK	2014(16);
562	DATGNVNDTIVTELTnCTSAACK	2007(72); 2009(62); 2012(50); 2014(22); 2015(1); 2015(12); 2015(unpublished);2007(unpublished);2007;

Sequence

1	11	21	31	41	51	61	71	81	91
MEPRPTAPSS	GAPGLAGVGE	TPSAAALAAA	RVELPGTAVP	SVPEDAAPAS	RDGGGVRDEG	PAAAGDGLGR	PLGPTPSQSR	FQVDLVSENA	GRAAAAAAAA
101	111	121	131	141	151	161	171	181	191
AAAAAAAGAG	AGAKQTPADG	EASGESEPAK	GSEEAKGRFR	VNFVDPAASS	SAEDSLSDAA	GVGVDGPNVS	FQNGGDTVLS	EGSSLHSGGG	GGSGHHQHYY
201	211	221	231	241	251	261	271	281	291
YDTHTNTYYL	RTFGHNTMDA	VPRIDHYRHT	AAQLGEKLLR	PSLAELHDEL	EKEPFEDGFA	NGEESTPTRD	AVVTYTAESK	GVVKFGWIKG	VLVRCMLNIW
301	311	321	331	341	351	361	371	381	391
GVMLFIRLSW	IVGQAGIGLS	VLVIMMATVV	TTITGLSTSA	IATNGFVRGG	GAYYLISRSL	GPEFGGAIGL	IFAFANAVAV	AMYVVGFAET	VVELLKEHSI
401	411	421	431	441	451	461	471	481	491
LMIDEINDIR	IIGAITVVIL	LGISVAGMEW	EAKAQIVLLV	ILLLAIGDFV	IGTFIPLESK	KPKGFFGYKS	EIFNENFGPD	FREEETFFSV	FAIFFPAATG
501	511	521	531	541	551	561	571	581	591
ILAGANISGD	LADPQSAIPK	GTLLAILITT	LVYVGIAVSV	GSCVVRDATG	NVNDTIVTEL	TNCTSAACKL	NFDFSSCESS	PCSYGLMNNF	QVMSMVSGFT
601	611	621	631	641	651	661	671	681	691
PLISAGIFSA	TLSSALASLV	SAPKIFQALC	KDNIYPAFQM	FAKGYGKNNE	PLRGYILTFL	IALGFILIAE	LNVIAPIISN	FFLASYALIN	FSVFHASLAK
701	711	721	731	741	751	761	771	781	791
SPGWRPAFKY	YNMWISLLGA	ILCCIVMFVI	NWWAALLTYV	IVLGLYIYVT	YKKPDVNWGS	STQALTYLNA	LQHSIRLSGV	EDHVKNFRPQ	CLVMTGAPNS
801	811	821	831	841	851	861	871	881	891
RPALLHLVHD	FTKNVGLMIC	GHVHMGPRRQ	AMKEMSIDQA	KYQRWLIKNK	MKAFYAPVHA	DDLREGAQYL	MQAAGLGRMK	PNTLVLGFKK	DWLQADMRDV
901	911	921	931	941	951	961	971	981	991
DMYINLFHDA	FDIQYGVVVI	RLKEGLDISH	LQGQEELLSS	QEKSPGTKDV	VVSVEYSKKS	DLDTSKPLSE	KPITHKVEEE	DGKTATQPLL	KKESKGPIVP
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
LNVADQKLLE	ASTQFQKKQG	KNTIDVWWLF	DDGGLTLLIP	YLLTTKKKWK	DCKIRVFIGG	KINRIDHDRR	AMATLLSKFR	IDFSDIMVLG	DINTKPKKEN
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
IIAFEEIIEP	YRLHEDDKEQ	DIADKMKEDE	PWRITDNELE	LYKTKTYRQI	RLNELLKEHS	STANIIVMSL	PVARKGAVSS	ALYMAWLEAL	SKDLPPILLV
1201	1211
RGNHQSVLTF	YS

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
12	Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22	Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50	Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
62	Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
72	Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.