Detail


Protein	Afadin
Gene	MLLT4
Status	Reviewed
Source	HEK293T cell line (embryonic kidney); OVCAR-3 cell line (Ovarian cancer)
Years	2011-2015

Site	Identified Peptides	Year(Publication ID)
1263	nHSSIAIQR	2015(1);
1263	YEEKPHMHTDSnHSSIAIQR	2011(57);

Sequence

1	11	21	31	41	51	61	71	81	91
MSAGGRDEER	RKLADIIHHW	NANRLDLFEI	SQPTEDLEFH	GVMRFYFQDK	AAGNFATKCI	RVSSTATTQD	VIETLAEKFR	PDMRMLSSPK	YSLYEVHVSG
101	111	121	131	141	151	161	171	181	191
ERRLDIDEKP	LVVQLNWNKD	DREGRFVLKN	ENDAIPPKKA	QSNGPEKQEK	EGVIQNFKRT	LSKKEKKEKK	KREKEALRQA	SDKDDRPFQG	EDVENSRLAA
201	211	221	231	241	251	261	271	281	291
EVYKDMPETS	FTRTISNPEV	VMKRRRQQKL	EKRMQEFRSS	DGRPDSGGTL	RIYADSLKPN	IPYKTILLST	TDPADFAVAE	ALEKYGLEKE	NPKDYCIARV
301	311	321	331	341	351	361	371	381	391
MLPPGAQHSD	EKGAKEIILD	DDECPLQIFR	EWPSDKGILV	FQLKRRPPDH	IPKKTKKHLE	GKTPKGKERA	DGSGYGSTLP	PEKLPYLVEL	SPGRRNHFAY
401	411	421	431	441	451	461	471	481	491
YNYHTYEDGS	DSRDKPKLYR	LQLSVTEVGT	EKLDDNSIQL	FGPGIQPHHC	DLTNMDGVVT	VTPRSMDAET	YVEGQRISET	TMLQSGMKVQ	FGASHVFKFV
501	511	521	531	541	551	561	571	581	591
DPSQDHALAK	RSVDGGLMVK	GPRHKPGIVQ	ETTFDLGGDI	HSGTALPTSK	STTRLDSDRV	SSASSTAERG	MVKPMIRVEQ	QPDYRRQESR	TQDASGPELI
601	611	621	631	641	651	661	671	681	691
LPASIEFRES	SEDSFLSAII	NYTNSSTVHF	KLSPTYVLYM	ACRYVLSNQY	RPDISPTERT	HKVIAVVNKM	VSMMEGVIQK	QKNIAGALAF	WMANASELLN
701	711	721	731	741	751	761	771	781	791
FIKQDRDLSR	ITLDAQDVLA	HLVQMAFKYL	VHCLQSELNN	YMPAFLDDPE	ENSLQRPKID	DVLHTLTGAM	SLLRRCRVNA	ALTIQLFSQL	FHFINMWLFN
801	811	821	831	841	851	861	871	881	891
RLVTDPDSGL	CSHYWGAIIR	QQLGHIEAWA	EKQGLELAAD	CHLSRIVQAT	TLLTMDKYAP	DDIPNINSTC	FKLNSLQLQA	LLQNYHCAPD	EPFIPTDLIE
901	911	921	931	941	951	961	971	981	991
NVVTVAENTA	DELARSDGRE	VQLEEDPDLQ	LPFLLPEDGY	SCDVVRNIPN	GLQEFLDPLC	QRGFCRLIPH	TRSPGTWTIY	FEGADYESHL	LRENTELAQP
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
LRKEPEIITV	TLKKQNGMGL	SIVAAKGAGQ	DKLGIYVKSV	VKGGAADVDG	RLAAGDQLLS	VDGRSLVGLS	QERAAELMTR	TSSVVTLEVA	KQGAIYHGLA
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
TLLNQPSPMM	QRISDRRGSG	KPRPKSEGFE	LYNNSTQNGS	PESPQLPWAE	YSEPKKLPGD	DRLMKNRADH	RSSPNVANQP	PSPGGKSAYA	SGTTAKITSV
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
STGNLCTEEQ	TPPPRPEAYP	IPTQTYTREY	FTFPASKSQD	RMAPPQNQWP	NYEEKPHMHT	DSNHSSIAIQ	RVTRSQEELR	EDKAYQLERH	RIEAAMDRKS
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
DSDMWINQSS	SLDSSTSSQE	HLNHSSKSVT	PASTLTKSGP	GRWKTPAAIP	ATPVAVSQPI	RTDLPPPPPP	PPVHYAGDFD	GMSMDLPLPP	PPSANQIGLP
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
SAQVAAAERR	KREEHQRWYE	KEKARLEEER	ERKRREQERK	LGQMRTQSLN	PAPFSPLTAQ	QMKPEKPSTL	QRPQETVIRE	LQPQQQPRTI	ERRDLQYITV
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
SKEELSSGDS	LSPDPWKRDA	KEKLEKQQQM	HIVDMLSKEI	QELQSKPDRS	AEESDRLRKL	MLEWQFQKRL	QESKQKDEDD	EEEEDDDVDT	MLIMQRLEAE
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
RRARLQDEER	RRQQQLEEMR	KREAEDRARQ	EEERRRQEEE	RTKRDAEEKR	RQEEGYYSRL	EAERRRQHDE	AARRLLEPEA	PGLCRPPLPR	DYEPPSPSPA
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
PGAPPPPPQR	NASYLKTQVL	SPDSLFTAKF	VAYNEEEEEE	DCSLAGPNSY	PGSTGAAVGA	HDACRDAKEK	RSKSQDADSP	GSSGAPENLT	FKERQRLFSQ
1801	1811	1821
GQDVSNKVKA	SRKLTELENE	LNTK

UniProtKB-P55196

Glycosites

Sequence

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
57	Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.