Contains      

UniProtKB-P55290

Protein Cadherin-13
Gene CDH13
Status Reviewed
Source Bladder stromal cell line; Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; FTC-133 Cell Line (Thyroid Cance); Hela cell line (cervical cancer); Liver; Liver tumor (HCC); Ovarian tumor; Pancreatic islets; Plasma; Prostate; Prostate stromal cell line; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Urine; hepatocarcinoma ascites syngeneic cell lines (Liver); plasma; umbilical vein Endothelial Cells
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
52 DQSILnLTFSDCK 2014(33);
52 IEDQSILnLTFSDCK 2007(unpublished);
52 VFHINQPAEFIEDQSILnLTFSDCK 2012(52); 2013(45); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
52 VFHINQPAEFIEDQSILnLTFSDCKGNDK 2013(45);
86 nITAVGK 2007(44); 2013(10); 2015(unpublished);2015(unpublished);2007;2007;
86 VNSDGGLVALRnITAVGK 2005(81); 2015(unpublished);2015(unpublished);
382 GAVGVIVnLTVEDKDDPTTGAWR 2014(33);
500 TVnATDPDSL 2014(33);
500 TVnATDPDSLQH 2014(33);
500 LTVnATDPDSLQH 2014(33);
500 TVnATDPDSLQHQ 2014(33);
500 LTVnATDPDSLQHQ 2014(33);
500 TVnATDPDSLQHQTIR 2014(33);
500 LTVnATDPDSLQHQTIR 2014(33);
500 TVnATDPDSLQHQTIRY 2014(33);
500 LTVnATDPDSLQHQTIRY 2014(33);
500 QEDISVGSVIITVnATDPDSIQHQTIR 2014(32); 2014(32);
500 QEDLSVGSVLLTVnATDPDSLQHQTIR 2005( 81); 2007(62); 2009(52); 2012(45); 2013(14); 2014(35); 2014(33); 2014(8); 2015(10); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;2007;
530 NINPInGTVDTTAVL 2014(33);
530 LNINPInGTVDTTAVL 2014(33);
530 NINPInGTVDTTAVLDR 2014(33);
530 LNINPInGTVDTTAVLDR 2014(33);
530 DPAGWLNINPInGTVDTTAVLDR 2012(52); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(8); 2015(10);
530 KDPAGWLNINPInGTVDTTAVLDR 2014(33);
530 YSVYKDPAGWLNINPInGTVDTTAVLDR 2009(64); 2012(52); 2014(33); 2015(unpublished);2015(12);
598 nLSVVILGASDK 2012(52); 2014(22); 2014(33); 2015(8); 2015(10); 2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;
598 nLSVVILGASDKD 2014(33);
598 VCDDAKnISVVII 2013(37);
598 IYPTVAEVCDDAKnL 2014(33);
598 VCDDAKnLSVVILGASDK 2014(33);
598 nLSVVILGASDKDLHPNTDPFK 2012(52); 2014(33); 2015(unpublished);
598 nLSVVILGASDKDLHPNTDPFKFE 2014(33);
598 VCDDAKnLSVVILGASDKDLHPNTDPFK 2014(33);
598 PTVAEVCDDAKnLSVVILGASDKDLHPNTDPFKF 2014(33);
638 InNTHALVSLL 2014(33);
638 KISKInNTHAL 2014(33);
638 WKISKInNTHAIV 2013(37);
638 KISKInNTHALVSL 2014(33);
638 InNTHALVSLLQNLNK 2005( 81); 2007(64); 2009(65); 2009(65); 2009(58); 2011(48); 2012(52); 2012(53); 2012(34); 2013(42); 2013(45); 2013(45); 2013(29); 2014(33); 2014(10); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;2007;
638 ISKInNTHALVSLLQNLNK 2014(33); 2015(12);
671 DSGKPPMTnITDLR 2012(3);
671 NLPIMVTDSGKPPMTnITDL 2014(33);
671 NLPIMVTDSGKPPMTnITDLR 2014(33);
671 ANYNLPIMVTDSGKPPMTnITDLR 2007(62); 2009(48); 2012(52); 2012(34); 2013(13); 2014(14); 2014(33); 2014(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;

Sequence

1112131415161718191
MQPRTPLVLCVLLSQVLLLTSAEDLDCTPGFQQKVFHINQPAEFIEDQSILNLTFSDCKGNDKLRYEVSSPYFKVNSDGGLVALRNITAVGKTLFVHART
101111121131141151161171181191
PHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPERSKFRLTGKGVDQEPKGIFRINENTGSVSVT
201211221231241251261271281291
RTLDREVIAVYQLFVETTDVNGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFY
301311321331341351361371381391
IDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIMIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAY
401411421431441451461471481491
TIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTRQEDLSVGSVLLTVN
501511521531541551561571581591
ATDPDSLQHQTIRYSVYKDPAGWLNINPINGTVDTTAVLDRESPFVDNSVYTALFLAIDSGNPPATGTGTLLITLEDVNDNAPFIYPTVAEVCDDAKNLS
601611621631641651661671681691
VVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCRNSKVDCNAAGALRFSLP
701711
SVLLLSLFSLACL

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
29Weng Y, Qu Y, Jiang H, Wu Q, Zhang L, Yuan H, Zhou Y, Zhang X, Zhang Y: An integrated sample pretreatment platform for quantitative N-glycoproteome analysis with combination of on-line glycopeptide enrichment, deglycosylation and dimethyl labeling. Analytica Chimica Acta 2014, 833:1-8.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.
81Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of Proteome Research 2005, 4:2070-2080.