Contains      

UniProtKB-P56199

Protein Integrin alpha-1
Gene ITGA1
Status Reviewed
Source 22Rv1 cell line (prostate cancer); 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Lymphocytes; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Urine; ovarian tumor
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
74 nRTGDVY 2014(33);
74 VGQPKnRTGDVY 2014(33);
74 GKWVLIGSPLVGQPKnR 2014(33);
74 VLIGSPLVGQPKnRTGDVY 2014(33);
100 LDLPVnTSIPN 2009(64);
100 DLPVnTSIPNVTE 2014(33);
100 LDLPVnTSIPNVTE 2014(33);
100 LDLPVnTSIPNVTEV 2014(33);
100 IDIPVnTSIPNVTEVK 2014(32); 2014(32);
100 LDLPVnTSIPNVTEVK 2009(64); 2011(54); 2012(50); 2012(50); 2012(52); 2014(14); 2014(22); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
100 LDLPVnTSIPNVTEVKE 2014(33);
100 DLPVnTSIPNVTEVKENMTF 2014(33);
100 DLPVnTSIPNVTEVKENMTFGSTL 2014(33);
105 DLPVNTSIPnVTE 2014(33);
105 LDLPVNTSIPnVTE 2014(33);
105 LDLPVNTSIPnVTEV 2014(33);
105 IDIPVNTSIPnVTEVK 2014(32); 2014(32);
105 LDLPVNTSIPnVTEVK 2009(64); 2011(54); 2012(50); 2012(50); 2012(52); 2014(14); 2014(22); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
105 LDLPVNTSIPnVTEVKE 2014(33);
105 DLPVNTSIPnVTEVKENMTF 2014(33);
105 DLPVNTSIPnVTEVKENMTFGSTL 2014(33);
112 EnMTFGSTL 2014(33);
112 VKEnMTFGST 2014(33);
112 EnMTFGSTLVTNPN 2014(33);
112 EnMTFGSTLVTNPNGGF 2014(33);
112 EnMTFGSTLVTNPNGGFL 2014(33);
112 DLPVNTSIPNVTEVKEnMTF 2014(33);
112 DLPVNTSIPNVTEVKEnMTFGSTL 2014(33);
112 EnMTFGSTLVTNPNGGFLACGPLY 2014(33);
112 nMTFGSTLVTNPNGGFLACGPLYAYR 2014(33);
112 EnMTFGSTIVTNPNGGFIACGPIYAYR 2014(32); 2014(32);
112 EnMTFGSTLVTNPNGGFLACGPLYAYR 2014(33); 2015(unpublished);2015;2007;
217 GEnVTHEF 2014(33);
217 YGEnVTHE 2014(33);
217 GEnVTHEFN 2014(33);
217 QYGEnVTHE 2014(33);
217 nVTHEFNLNK 2015(1);
217 GEnVTHEFNLN 2014(33);
217 IVQYGEnVTHE 2009(64);
217 GEnVTHEFNLNK 2014(33);
217 IVQYGEnVTHEF 2014(33);
217 GEnVTHEFNLNKY 2014(33);
217 VGIVQYGEnVTHEF 2014(33);
217 QTQVGIVQYGEnVTHE 2014(33);
217 QTQVGIVQYGEnVTHEF 2014(33);
217 QTQVGIVQYGEnVTHEFNINK 2014(16); 2014(32);
217 QTQVGIVQYGEnVTHEFNLNK 2009(67); 2012(50); 2014(33); 2015(1); 2015(2); 2015(9); 2015(unpublished);2015;2007;
217 MDIGPKQTQVGIVQYGEnVTHEF 2014(33);
217 LNDLLERMDIGPKQTQVGIVQYGEnVTHEF 2014(33);
317 GnISTEK 2014(32); 2014(32);
317 GnLSTEK 2014(22); 2015(unpublished);
317 NRGnLSTEKF 2014(33);
317 GnLSTEKFVEE 2014(33);
317 GnLSTEKFVEEIK 2014(33); 2015(2); 2015(12);
317 GSYNRGnLSTEKF 2014(33);
317 NRGnLSTEKFVEEIK 2014(33);
317 nLSTEKFVEEIKSIASEPTEK 2015(1);
341 HFFnVSDE 2014(33);
341 HFFnVSDEL 2014(33);
341 KHFFnVSDE 2014(33);
341 HFFnVSDEIAIVTIVK 2014(32);
341 HFFnVSDELALVTIVK 2009(64); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
341 SIASEPTEKHFFnVSDE 2014(33);
341 SIASEPTEKHFFnVSDELALVTIVK 2015(2);
402 DWnGTVVM 2014(33);
402 DWnGTVVMQ 2014(33);
402 DWnGTVVMQK 2014(19); 2014(33);
402 DWnGTVVMQKA 2014(33);
402 YDWnGTVVMQK 2014(33);
402 AYDWnGTVVMQK 2014(33);
402 DWnGTVVMQKAS 2014(33);
402 DWnGTVVMQKASQ 2014(33);
402 GAYDWnGTVVMQK 2014(33);
402 GAVGAYDWnGTVVMQK 2014(33);
402 LGAVGAYDWnGTVVMQK 2014(33);
402 DWnGTVVMQKASQIIIPR 2014(33);
402 VMLGAVGAYDWnGTVVMQK 2014(33);
402 DWnGTVVMQKASQIIIPRNTTF 2014(33);
402 SQDWVMLGAVGAYDWnGTVVMQK 2014(33);
402 SAHYSQDWVMLGAVGAYDWnGTVVMQK 2014(33);
418 nTTFNVE 2014(33);
418 IIIPRnTTF 2014(33);
418 nTTFNVESTK 2009(64); 2011(55); 2012(50); 2013(40); 2014(14); 2014(15); 2014(16); 2014(18); 2014(19); 2014(22); 2014(33); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
418 QIIIPRnTTF 2014(33);
418 nTTFNVESTKK 2014(14); 2014(15); 2014(33); 2015(unpublished);
418 ASQIIIPRnTTF 2014(33);
418 KASQIIIPRnTTF 2014(33);
418 QKASQIIIPRnTTF 2014(33);
418 DWNGTVVMQKASQIIIPRnTTF 2014(33);
460 nHTGQVIIY 2014(33);
460 YnHTGQVIIY 2014(33);
460 YnHTGQVIIYR 2007(62); 2009(64); 2009(67); 2009(50); 2012(34); 2013(34); 2013(14); 2014(16); 2014(19); 2014(22); 2014(32); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;2007;
460 IAGQPRYnHTGQ 2014(33);
460 AGQPRYnHTGQVI 2013(37);
460 IAGQPRYnHTGQV 2009(64);
460 IAGQPRYnHTGQVIIY 2014(33);
460 YIAGQPRYnHTGQVIIY 2014(33);
532 ALnQTRF 2014(33);
532 ALnQTRFE 2014(33);
532 LnQTRFEY 2014(33);

Sequence

1112131415161718191
MAPRPRARPGVAVACCWLLTVVLRCCVSFNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVN
101111121131141151161171181191
TSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERM
201211221231241251261271281291
DIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDE
301311321331341351361371381391
NIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYD
401411421431441451461471481491
WNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDID
501511521531541551561571581591
KDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHG
601611621631641651661671681691
GAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINA
701711721731741751761771781791
TVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHE
801811821831841851861871881891
YIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVT
901911921931941951961971981991
FKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPEL
1001101110211031104110511061107110811091
KLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSS
11011111112111311141115111611171
LNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
55Tian Y, Bova GS, Zhang H: Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer. Analytical chemistry 2011, 83:7013-7019.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.